A robust expression and purification method for production of SpCas9-GFP-MBP fusion protein for In vitro applications
Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant Streptococcus pyogenes Cas9 (SpCas9) at some point during their application, for instance, for in vitro validation of single guide RNAs (S...
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| Other Authors: | , |
| Format: | article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/20.500.12008/41377 |
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