A robust expression and purification method for production of SpCas9-GFP-MBP fusion protein for In vitro applications

Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant Streptococcus pyogenes Cas9 (SpCas9) at some point during their application, for instance, for in vitro validation of single guide RNAs (S...

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Bibliographic Details

Main Author:	Fleitas, Andrea Luciana (author)
Other Authors:	Señorale, Mario (author), Vidal, Sabina (author)
Format:	article
Language:	English
Published:	2022
Subjects:	CIRSPR/Cas Expression Purification Immobilized metal affinity chromatography Ion exchange
Online Access:	https://hdl.handle.net/20.500.12008/41377
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