Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces
Grape pomaces have a wide and diverse antioxidant phenolics composition. Six Uruguayan red grape pomaces were evaluated in their phenolics composition, antioxidant capacity, and anti-inflammatory properties. Not only radical scavenging methods as DPPH· and ABTS·+ were employed but also ORAC and FRAP...
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| Materyal Türü: | article |
| Dil: | İngilizce |
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2023
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| Konular: | |
| Online Erişim: | https://hdl.handle.net/20.500.12381/3239 https://doi.org/10.3390/molecules28093909 |
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| _version_ | 1868890156638928896 |
|---|---|
| author | Fariña, Emiliana |
| author2 | Daghero, Hellen Bollati-Fogolín, Mariela Boido, Eduardo Cantero, Jorge Moncada-Basualto, Mauricio Olea-Azar, Claudio Polticelli, Fabio Paulino, Margot |
| author2_role | author author author author author author author author |
| author_browse | Boido, Eduardo Bollati-Fogolín, Mariela Cantero, Jorge Daghero, Hellen Fariña, Emiliana Moncada-Basualto, Mauricio Olea-Azar, Claudio Paulino, Margot Polticelli, Fabio |
| author_facet | Fariña, Emiliana Daghero, Hellen Bollati-Fogolín, Mariela Boido, Eduardo Cantero, Jorge Moncada-Basualto, Mauricio Olea-Azar, Claudio Polticelli, Fabio Paulino, Margot |
| author_role | author |
| collection | IPMON en REDI |
| dc.creator.none.fl_str_mv | Fariña, Emiliana Daghero, Hellen Bollati-Fogolín, Mariela Boido, Eduardo Cantero, Jorge Moncada-Basualto, Mauricio Olea-Azar, Claudio Polticelli, Fabio Paulino, Margot |
| dc.date.none.fl_str_mv | 2023-06-05T14:29:02Z 2023-06-05T14:29:02Z 2023-05-05 |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/3239 ALI_2_2018_1_149574 https://doi.org/10.3390/molecules28093909 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI, Basel, Switzerland. |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Reconocimiento 4.0 Internacional. (CC BY) |
| dc.source.none.fl_str_mv | Molecules reponame:IPMON en REDI instname:Institut Pasteur de Montevideo instacron:Institut Pasteur de Montevideo |
| dc.subject.none.fl_str_mv | ABTS·+ DPPH· ESR FRAP NF-κB ORAC Antioxidants Grape pomace Molecular docking Phenols Ciencias Naturales y Exactas Otras Ciencias Naturales |
| dc.title.none.fl_str_mv | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | Grape pomaces have a wide and diverse antioxidant phenolics composition. Six Uruguayan red grape pomaces were evaluated in their phenolics composition, antioxidant capacity, and anti-inflammatory properties. Not only radical scavenging methods as DPPH· and ABTS·+ were employed but also ORAC and FRAP analyses were applied to assess the antioxidant potency of the extracts. The antioxidant reactivity of all extracts against hydroxyl radicals was assessed with ESR. The phenol profile of the most bioactive extract was analyzed by HPLC-MS, and a set of 57 structures were determined. To investigate the potential anti-inflammatory activity of the extracts, Nuclear Factor kappa-B (NF-κB) modulation was evaluated in the human colon cancer reporter cell line (HT-29-NF-κB-hrGFP). Our results suggest that Tannat grapes pomaces have higher phenolic content and antioxidant capacity compared to Cabernet Franc. These extracts inhibited TNF-alpha mediated NF-κB activation and IL-8 production when added to reporter cells. A molecular docking study was carried out to rationalize the experimental results allowing us to propose the proactive interaction between the NF-κB, the grape extracts phenols, and their putative anti-inflammatory bioactivity. The present findings show that red grape pomace constitutes a sustainable source of phenolic compounds, which may be valuable for pharmaceutical, cosmetic, and food industry applications. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_a67b4419fb57714be5482012d1587405 |
| identifier_str_mv | ALI_2_2018_1_149574 |
| instacron_str | Institut Pasteur de Montevideo |
| institution | Institut Pasteur de Montevideo |
| instname_str | Institut Pasteur de Montevideo |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/3239 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | MDPI, Basel, Switzerland. |
| reponame_str | IPMON en REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Reconocimiento 4.0 Internacional. (CC BY) |
| spelling | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape PomacesFariña, EmilianaDaghero, HellenBollati-Fogolín, MarielaBoido, EduardoCantero, JorgeMoncada-Basualto, MauricioOlea-Azar, ClaudioPolticelli, FabioPaulino, MargotABTS·+DPPH·ESRFRAPNF-κBORACAntioxidantsGrape pomaceMolecular dockingPhenolsCiencias Naturales y ExactasOtras Ciencias NaturalesGrape pomaces have a wide and diverse antioxidant phenolics composition. Six Uruguayan red grape pomaces were evaluated in their phenolics composition, antioxidant capacity, and anti-inflammatory properties. Not only radical scavenging methods as DPPH· and ABTS·+ were employed but also ORAC and FRAP analyses were applied to assess the antioxidant potency of the extracts. The antioxidant reactivity of all extracts against hydroxyl radicals was assessed with ESR. The phenol profile of the most bioactive extract was analyzed by HPLC-MS, and a set of 57 structures were determined. To investigate the potential anti-inflammatory activity of the extracts, Nuclear Factor kappa-B (NF-κB) modulation was evaluated in the human colon cancer reporter cell line (HT-29-NF-κB-hrGFP). Our results suggest that Tannat grapes pomaces have higher phenolic content and antioxidant capacity compared to Cabernet Franc. These extracts inhibited TNF-alpha mediated NF-κB activation and IL-8 production when added to reporter cells. A molecular docking study was carried out to rationalize the experimental results allowing us to propose the proactive interaction between the NF-κB, the grape extracts phenols, and their putative anti-inflammatory bioactivity. The present findings show that red grape pomace constitutes a sustainable source of phenolic compounds, which may be valuable for pharmaceutical, cosmetic, and food industry applications.Agencia Nacional de Investigación e InnovaciónPrograma de Desarrollo de Ciencias BásicasComisión Sectorial de Investigación CientíficaMECESUP, ChileItalian Ministry of University and Research (MIUR), grants “Dipartimenti di Eccellenza”MDPI, Basel, Switzerland.2023-06-05T14:29:02Z2023-06-05T14:29:02Z2023-05-05Artículoinfo:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/20.500.12381/3239ALI_2_2018_1_149574https://doi.org/10.3390/molecules28093909Moleculesreponame:IPMON en REDIinstname:Institut Pasteur de Montevideoinstacron:Institut Pasteur de MontevideoengAcceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento 4.0 Internacional. (CC BY)oai:redi.anii.org.uy:20.500.12381/32392026-06-16T05:20:25Z |
| spellingShingle | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces Fariña, Emiliana ABTS·+ DPPH· ESR FRAP NF-κB ORAC Antioxidants Grape pomace Molecular docking Phenols Ciencias Naturales y Exactas Otras Ciencias Naturales |
| status_str | publishedVersion |
| title | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| title_full | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| title_fullStr | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| title_full_unstemmed | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| title_short | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| title_sort | Antioxidant Capacity and NF-kB-Mediated Anti-Inflammatory Activity of Six Red Uruguayan Grape Pomaces |
| topic | ABTS·+ DPPH· ESR FRAP NF-κB ORAC Antioxidants Grape pomace Molecular docking Phenols Ciencias Naturales y Exactas Otras Ciencias Naturales |
| url | https://hdl.handle.net/20.500.12381/3239 https://doi.org/10.3390/molecules28093909 |