Activation of the NLRP3 inflammasome by particles from the Echinococcus granulosus laminated layer
The interaction of dendritic cells and macrophages with a variety ofrigid noncellular particles triggers activation of the NLRP3 inflammasome and conse-quent secretion of interleukin 1 (IL-1 ). Noncellular particles can also be generatedin the context of helminth infection, since these large pathoge...
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| Altres autors: | , , , |
| Format: | article |
| Idioma: | anglès |
| Publicat: |
2020
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| Matèries: | |
| Accés en línia: | https://hdl.handle.net/20.500.12008/31665 |
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| Sumari: | The interaction of dendritic cells and macrophages with a variety ofrigid noncellular particles triggers activation of the NLRP3 inflammasome and conse-quent secretion of interleukin 1 (IL-1 ). Noncellular particles can also be generatedin the context of helminth infection, since these large pathogens often shed theiroutermost structures during growth and/or molting. One such structure is the mas-sive, mucin-based, soft, flexible laminated layer (LL), which protects the larval stagesof cestodes of the genusEchinococcus. We show that particles from theEchinococcusgranulosusLL (pLL) trigger NLRP3- and caspase-1-dependent IL-1 in lipopolysaccha-ride (LPS)-primed mouse bone marrow-derived dendritic cells (BMDC). This responsecan be elicited by pLL too large for phagocytosis and nonetheless requires actin dy-namics, Syk, and phosphatidylinositol 3-kinase (PI3K). These three requirements hadalready been observed in our previous study on the alteration by pLL of CD86,CD40, IL-10, and IL-12 responses to LPS in BMDC; however, we now show that thesealterations are independent of NLRP3 and caspase-1. In other words, an initial inter-action with particles requiring actin dynamics, Syk, and PI3K, but not phagocytosis,elicits both NLRP3-dependent and NLRP3-independent responses. Intraperitoneal in-jection of pLL induced IL-1 , suggesting that contact with LL materials induces IL-1 in theE. granulosusinfection setting. Our results extend our understanding of NLRP3inflammasome activation by noncellular particulate materials both to helminth-derived materials and to flexible/soft materials. |
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