Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice
Myelination of the peripheral nervous system requires Schwann cells (SC) differentiation into the myelinating phenotype. The peripheral myelin protein-22 (PMP22) is an integral membrane glycoprotein, expressed in SC. It was initially described as a growth arrest-specific (gas3) gene product, up-regu...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2022
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/38338 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890175477645312 |
|---|---|
| author | Di Tomaso, María Vittoria |
| author2 | Vázquez Alberdi, Lucía Olsson, Daniela Cancela Bruno, Saira Fernández Constenla, Anabel Sonia Rosillo Martí, Juan Carlos Reyes Ábalos, Ana Laura Álvarez Zabaleta, Magdalena Calero, Miguel Kun González, Alejandra E. |
| author2_role | author author author author author author author author author |
| author_browse | Calero, Miguel Cancela Bruno, Saira Di Tomaso, María Vittoria Fernández Constenla, Anabel Sonia Kun González, Alejandra E. Olsson, Daniela Reyes Ábalos, Ana Laura Rosillo Martí, Juan Carlos Vázquez Alberdi, Lucía Álvarez Zabaleta, Magdalena |
| author_facet | Di Tomaso, María Vittoria Vázquez Alberdi, Lucía Olsson, Daniela Cancela Bruno, Saira Fernández Constenla, Anabel Sonia Rosillo Martí, Juan Carlos Reyes Ábalos, Ana Laura Álvarez Zabaleta, Magdalena Calero, Miguel Kun González, Alejandra E. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Di Tomaso María Vittoria, IIBCE Vázquez Alberdi Lucía, IIBCE Olsson Daniela, IIBCE Cancela Bruno Saira, IIBCE Fernández Constenla Anabel Sonia, IIBCE Rosillo Martí Juan Carlos, IIBCE Reyes Ábalos Ana Laura, IIBCE Álvarez Zabaleta Magdalena, IIBCE Calero Miguel Kun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. |
| dc.creator.none.fl_str_mv | Di Tomaso, María Vittoria Vázquez Alberdi, Lucía Olsson, Daniela Cancela Bruno, Saira Fernández Constenla, Anabel Sonia Rosillo Martí, Juan Carlos Reyes Ábalos, Ana Laura Álvarez Zabaleta, Magdalena Calero, Miguel Kun González, Alejandra E. |
| dc.date.none.fl_str_mv | 2022 2023-07-21T18:03:51Z 2023-07-21T18:03:51Z |
| dc.format.none.fl_str_mv | 23 h. application/pdf |
| dc.identifier.none.fl_str_mv | Di Tomaso, M, Vázquez Alberdi, L, Olsson, D, [y otros autores]. "Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice". Biomolecules. [en línea] 2022, 12(3), 456.23 h. DOI: 10.3390/biom12030456 2218-273X https://hdl.handle.net/20.500.12008/38338 10.3390/biom12030456 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Biomolecules, 2022, 12(3), 456. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | PMP22 Lamin B1 Trembler-J Schwann cells nuclei Colocalization-analysis |
| dc.title.none.fl_str_mv | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Myelination of the peripheral nervous system requires Schwann cells (SC) differentiation into the myelinating phenotype. The peripheral myelin protein-22 (PMP22) is an integral membrane glycoprotein, expressed in SC. It was initially described as a growth arrest-specific (gas3) gene product, up-regulated by serum starvation. PMP22 mutations were pathognomonic for human hereditary peripheral neuropathies, including the Charcot-Marie-Tooth disease (CMT). Trembler-J (TrJ) is a heterozygous mouse model carrying the same pmp22 point mutation as a CMT1E variant. Mutations in lamina genes have been related to a type of peripheral (CMT2B1) or central (autosomal dominant leukodystrophy) neuropathy. We explore the presence of PMP22 and Lamin B1 in Wt and TrJ SC nuclei of sciatic nerves and the colocalization of PMP22 concerning the silent heterochromatin (HC: DAPI-dark counterstaining), the transcriptionally active euchromatin (EC), and the nuclear lamina (H3K4m3 and Lamin B1 immunostaining, respectively). The results revealed that the number of TrJ SC nuclei in sciatic nerves was greater, and the SC volumes were smaller than those of Wt. The myelin protein PMP22 and Lamin B1 were detected in Wt and TrJ SC nuclei and predominantly in peripheral nuclear regions. The level of PMP22 was higher, and those of Lamin B1 lower in TrJ than in Wt mice. The level of PMP22 was higher, and those of Lamin B1 lower in TrJ than in Wt mice. PMP22 colocalized more with Lamin B1 and with the transcriptionally competent EC, than the silent HC with differences between Wt and TrJ genotypes. The results are discussed regarding the probable nuclear role of PMP22 and the relationship with TrJ neuropathy. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_b95d4880a2a62223c79a3bbbcd149f5f |
| identifier_str_mv | Di Tomaso, M, Vázquez Alberdi, L, Olsson, D, [y otros autores]. "Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice". Biomolecules. [en línea] 2022, 12(3), 456.23 h. DOI: 10.3390/biom12030456 2218-273X 10.3390/biom12030456 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/38338 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ MiceDi Tomaso, María VittoriaVázquez Alberdi, LucíaOlsson, DanielaCancela Bruno, SairaFernández Constenla, Anabel SoniaRosillo Martí, Juan CarlosReyes Ábalos, Ana LauraÁlvarez Zabaleta, MagdalenaCalero, MiguelKun González, Alejandra E.PMP22Lamin B1Trembler-JSchwann cells nucleiColocalization-analysisMyelination of the peripheral nervous system requires Schwann cells (SC) differentiation into the myelinating phenotype. The peripheral myelin protein-22 (PMP22) is an integral membrane glycoprotein, expressed in SC. It was initially described as a growth arrest-specific (gas3) gene product, up-regulated by serum starvation. PMP22 mutations were pathognomonic for human hereditary peripheral neuropathies, including the Charcot-Marie-Tooth disease (CMT). Trembler-J (TrJ) is a heterozygous mouse model carrying the same pmp22 point mutation as a CMT1E variant. Mutations in lamina genes have been related to a type of peripheral (CMT2B1) or central (autosomal dominant leukodystrophy) neuropathy. We explore the presence of PMP22 and Lamin B1 in Wt and TrJ SC nuclei of sciatic nerves and the colocalization of PMP22 concerning the silent heterochromatin (HC: DAPI-dark counterstaining), the transcriptionally active euchromatin (EC), and the nuclear lamina (H3K4m3 and Lamin B1 immunostaining, respectively). The results revealed that the number of TrJ SC nuclei in sciatic nerves was greater, and the SC volumes were smaller than those of Wt. The myelin protein PMP22 and Lamin B1 were detected in Wt and TrJ SC nuclei and predominantly in peripheral nuclear regions. The level of PMP22 was higher, and those of Lamin B1 lower in TrJ than in Wt mice. The level of PMP22 was higher, and those of Lamin B1 lower in TrJ than in Wt mice. PMP22 colocalized more with Lamin B1 and with the transcriptionally competent EC, than the silent HC with differences between Wt and TrJ genotypes. The results are discussed regarding the probable nuclear role of PMP22 and the relationship with TrJ neuropathy.ANII: FCE_1_2019_1_155539Di Tomaso María Vittoria, IIBCEVázquez Alberdi Lucía, IIBCEOlsson Daniela, IIBCECancela Bruno Saira, IIBCEFernández Constenla Anabel Sonia, IIBCERosillo Martí Juan Carlos, IIBCEReyes Ábalos Ana Laura, IIBCEÁlvarez Zabaleta Magdalena, IIBCECalero MiguelKun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.2023-07-21T18:03:51Z2023-07-21T18:03:51Z2022Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion23 h.application/pdfDi Tomaso, M, Vázquez Alberdi, L, Olsson, D, [y otros autores]. "Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice". Biomolecules. [en línea] 2022, 12(3), 456.23 h. DOI: 10.3390/biom120304562218-273Xhttps://hdl.handle.net/20.500.12008/3833810.3390/biom12030456reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengBiomolecules, 2022, 12(3), 456.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/383382026-04-14T10:10:08Z |
| spellingShingle | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice Di Tomaso, María Vittoria PMP22 Lamin B1 Trembler-J Schwann cells nuclei Colocalization-analysis |
| status_str | publishedVersion |
| title | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| title_full | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| title_fullStr | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| title_full_unstemmed | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| title_short | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| title_sort | Colocalization analysis of peripheral myelin protein-22 and lamin-B1 in the schwann cell nuclei of Wt and TrJ Mice |
| topic | PMP22 Lamin B1 Trembler-J Schwann cells nuclei Colocalization-analysis |
| url | https://hdl.handle.net/20.500.12008/38338 |