Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as F...
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| Hōputu: | article |
| Reo: | Ingarihi |
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2017
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| Urunga tuihono: | https://hdl.handle.net/20.500.12008/22412 |
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Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
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| _version_ | 1868890037383331840 |
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| author | McNulty, S. |
| author2 | Tort, José F. Rinaldi, Gabriel Fischer, K. Rosa, B.A. Smircich, Pablo Fontenla Martínez, Santiago Choi, Y. Tyagi, R. Hallsworth-Pepin, K. Mann, H.M. Kammili, L. Latham, P.S. Dell'Oca Runco, Nicolás Domínguez, María Fernanda Carmona García, Carlos Fischer, P.U. Brindley, P.J. Mitreva, M. |
| author2_role | author author author author author author author author author author author author author author author author author author |
| author_browse | Brindley, P.J. Carmona García, Carlos Choi, Y. Dell'Oca Runco, Nicolás Domínguez, María Fernanda Fischer, K. Fischer, P.U. Fontenla Martínez, Santiago Hallsworth-Pepin, K. Kammili, L. Latham, P.S. Mann, H.M. McNulty, S. Mitreva, M. Rinaldi, Gabriel Rosa, B.A. Smircich, Pablo Tort, José F. Tyagi, R. |
| author_facet | McNulty, S. Tort, José F. Rinaldi, Gabriel Fischer, K. Rosa, B.A. Smircich, Pablo Fontenla Martínez, Santiago Choi, Y. Tyagi, R. Hallsworth-Pepin, K. Mann, H.M. Kammili, L. Latham, P.S. Dell'Oca Runco, Nicolás Domínguez, María Fernanda Carmona García, Carlos Fischer, P.U. Brindley, P.J. Mitreva, M. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | McNulty S. Tort Jose F., Universidad de la República (Uruguay). Facultad de Medicina Rinaldi G. Fischer K. Rosa B.A. Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Fontenla Santiago, Universidad de la República (Uruguay). Facultad de Medicina Choi Y. Tyagi R. Hallsworth-Pepin K. Mann H.M. Kammili L. Latham P.S. Dell'Oca Runco Nicolás, Universidad de la República (Uruguay). Facultad de Medicina Dominguez Fernanda, Universidad de la República (Uruguay). Facultad de Medicina Carmona Carlos, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Fischer P.U. Brindley P.J. Mitreva M |
| dc.creator.none.fl_str_mv | McNulty, S. Tort, José F. Rinaldi, Gabriel Fischer, K. Rosa, B.A. Smircich, Pablo Fontenla Martínez, Santiago Choi, Y. Tyagi, R. Hallsworth-Pepin, K. Mann, H.M. Kammili, L. Latham, P.S. Dell'Oca Runco, Nicolás Domínguez, María Fernanda Carmona García, Carlos Fischer, P.U. Brindley, P.J. Mitreva, M. |
| dc.date.none.fl_str_mv | 2017 2019-11-18T16:14:04Z 2019-11-18T16:14:04Z |
| dc.format.none.fl_str_mv | 25 h. application/pdf |
| dc.identifier.none.fl_str_mv | MCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.1006537 1553-7404 https://hdl.handle.net/20.500.12008/22412 10.1371/journal.pgen.1006537 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | PLoS |
| dc.relation.none.fl_str_mv | PLoS Genetics, 2017, 13(1): e1006537 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Trematodos transmitidos por alimentos FBT Fasciola hepática Genética |
| dc.title.none.fl_str_mv | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered transkingdom interaction with nFh and the impact of both species on disease in ruminants and humans. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_4bdc40afa2ccbf5ca3579beffb064c0f |
| identifier_str_mv | MCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.1006537 1553-7404 10.1371/journal.pgen.1006537 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/22412 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher.none.fl_str_mv | PLoS |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu FeversMcNulty, S.Tort, José F.Rinaldi, GabrielFischer, K.Rosa, B.A.Smircich, PabloFontenla Martínez, SantiagoChoi, Y.Tyagi, R.Hallsworth-Pepin, K.Mann, H.M.Kammili, L.Latham, P.S.Dell'Oca Runco, NicolásDomínguez, María FernandaCarmona García, CarlosFischer, P.U.Brindley, P.J.Mitreva, M.Trematodos transmitidos por alimentosFBTFasciola hepáticaGenéticaFood borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered transkingdom interaction with nFh and the impact of both species on disease in ruminants and humans.PLoSMcNulty S.Tort Jose F., Universidad de la República (Uruguay). Facultad de MedicinaRinaldi G.Fischer K.Rosa B.A.Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFontenla Santiago, Universidad de la República (Uruguay). Facultad de MedicinaChoi Y.Tyagi R.Hallsworth-Pepin K.Mann H.M.Kammili L.Latham P.S.Dell'Oca Runco Nicolás, Universidad de la República (Uruguay). Facultad de MedicinaDominguez Fernanda, Universidad de la República (Uruguay). Facultad de MedicinaCarmona Carlos, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFischer P.U.Brindley P.J.Mitreva M2019-11-18T16:14:04Z2019-11-18T16:14:04Z2017Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion25 h.application/pdfMCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.10065371553-7404https://hdl.handle.net/20.500.12008/2241210.1371/journal.pgen.1006537reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengPLoS Genetics, 2017, 13(1): e1006537Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/224122026-04-14T10:09:21Z |
| spellingShingle | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers McNulty, S. Trematodos transmitidos por alimentos FBT Fasciola hepática Genética |
| status_str | publishedVersion |
| title | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| title_full | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| title_fullStr | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| title_full_unstemmed | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| title_short | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| title_sort | Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers |
| topic | Trematodos transmitidos por alimentos FBT Fasciola hepática Genética |
| url | https://hdl.handle.net/20.500.12008/22412 |