Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures
Preclinical research supports the benefits of pharmaceutical cannabis-based extracts for treating different medical conditions (e. g., epilepsy); however, their neuroprotective potential has not been widely investigated. In addition, there is still controversy about the impact of other factors in th...
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| Další autoři: | , , , , , , |
| Médium: | report |
| Jazyk: | angličtina |
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2023
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| Témata: | |
| On-line přístup: | https://hdl.handle.net/20.500.12381/3368 |
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| _version_ | 1868890233896960000 |
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| author | Echeverry, Carolina |
| author2 | Richeri, Analía Fagetti, Jimena Martínez, Gaby Prunell, Giselle Sánchez De Medina, Verónica Ferreiro, Carlos Scorza, Cecilia |
| author2_role | author author author author author author author |
| author_browse | Echeverry, Carolina Fagetti, Jimena Ferreiro, Carlos Martínez, Gaby Prunell, Giselle Richeri, Analía Scorza, Cecilia Sánchez De Medina, Verónica |
| author_facet | Echeverry, Carolina Richeri, Analía Fagetti, Jimena Martínez, Gaby Prunell, Giselle Sánchez De Medina, Verónica Ferreiro, Carlos Scorza, Cecilia |
| author_role | author |
| collection | IIBCE en REDI |
| dc.creator.none.fl_str_mv | Echeverry, Carolina Richeri, Analía Fagetti, Jimena Martínez, Gaby Prunell, Giselle Sánchez De Medina, Verónica Ferreiro, Carlos Scorza, Cecilia |
| dc.date.none.fl_str_mv | 2023-12-27T16:29:13Z 2023-12-27T16:29:13Z 2023-09-13 |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/3368 FCE_3_2020_1_162440 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | IBRO Neuroscience Reports |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Dedicación de Dominio Público 1.0 Universal. (CC0) |
| dc.source.none.fl_str_mv | IBRO Neuroscience Reports 15 (2023) S67–S466 reponame:IIBCE en REDI instname:Instituto de Investigaciones Biológicas Clemente Estable instacron:Instituto de Investigaciones Biológicas Clemente Estable |
| dc.subject.none.fl_str_mv | Cannabis sativa Epifractan XALEX Ciencias Médicas y de la Salud Medicina Básica Neurociencias |
| dc.title.none.fl_str_mv | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| dc.type.none.fl_str_mv | Reporte técnico info:eu-repo/semantics/report Publicado info:eu-repo/semantics/publishedVersion |
| description | Preclinical research supports the benefits of pharmaceutical cannabis-based extracts for treating different medical conditions (e. g., epilepsy); however, their neuroprotective potential has not been widely investigated. In addition, there is still controversy about the impact of other factors in the beneficial effect of these extracts (e.g., the entourage effect, and oil formulations). We evaluated the neuroprotective activity of Epifractan (EPI), a cannabis-based medicinal extract containing a high level of cannabidiol (CBD), components like terpenoids and flavonoids, and trace levels of Δ9-tetrahydrocannabinol and the acid form of CBD. Using primary cultures of cerebellar granule cells, we determined the ability of EPI to counteract the rotenone-induced neurotoxicity by analyzing cell viability and morphology of neurons and astrocytes by immunocytochemical assays. The effect of EPI was compared with XALEX, a plant-derived and highly purified CBD formulation (XAL), and pure CBD crystals (CBD). The results revealed that EPI induced a significant reduction in the rotenone-induced neurotoxicity in a wide range of concentrations without causing neurotoxicity per se. EPI showed a similar effect to XAL suggesting that no additive or synergistic interactions (i.e., entourage effect) between individual substances present in EPI occurred. In contrast, CBD crystals did show a different profile to EPI and XAL since a neurotoxic effect per se was observed at the higher concentrations assayed. Medium-chain triglyceride oil used in EPI formulation could explain this difference. Our data support a neuroprotective effect of EPI which may provide neuroprotection in different neurodegenerative processes. The results highlight the role of CBD as the active component of EPI but also support the need for an appropriate formulation to dilute pharmaceutical cannabis-based product |
| eu_rights_str_mv | openAccess |
| format | report |
| id | anni_f4e0f45e029fbca5ff3573899b7cf8dd |
| identifier_str_mv | FCE_3_2020_1_162440 |
| instacron_str | Instituto de Investigaciones Biológicas Clemente Estable |
| institution | Instituto de Investigaciones Biológicas Clemente Estable |
| instname_str | Instituto de Investigaciones Biológicas Clemente Estable |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/3368 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | IBRO Neuroscience Reports |
| reponame_str | IIBCE en REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Dedicación de Dominio Público 1.0 Universal. (CC0) |
| spelling | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell culturesEcheverry, CarolinaRicheri, AnalíaFagetti, JimenaMartínez, GabyPrunell, GiselleSánchez De Medina, VerónicaFerreiro, CarlosScorza, CeciliaCannabis sativaEpifractanXALEXCiencias Médicas y de la SaludMedicina BásicaNeurocienciasPreclinical research supports the benefits of pharmaceutical cannabis-based extracts for treating different medical conditions (e. g., epilepsy); however, their neuroprotective potential has not been widely investigated. In addition, there is still controversy about the impact of other factors in the beneficial effect of these extracts (e.g., the entourage effect, and oil formulations). We evaluated the neuroprotective activity of Epifractan (EPI), a cannabis-based medicinal extract containing a high level of cannabidiol (CBD), components like terpenoids and flavonoids, and trace levels of Δ9-tetrahydrocannabinol and the acid form of CBD. Using primary cultures of cerebellar granule cells, we determined the ability of EPI to counteract the rotenone-induced neurotoxicity by analyzing cell viability and morphology of neurons and astrocytes by immunocytochemical assays. The effect of EPI was compared with XALEX, a plant-derived and highly purified CBD formulation (XAL), and pure CBD crystals (CBD). The results revealed that EPI induced a significant reduction in the rotenone-induced neurotoxicity in a wide range of concentrations without causing neurotoxicity per se. EPI showed a similar effect to XAL suggesting that no additive or synergistic interactions (i.e., entourage effect) between individual substances present in EPI occurred. In contrast, CBD crystals did show a different profile to EPI and XAL since a neurotoxic effect per se was observed at the higher concentrations assayed. Medium-chain triglyceride oil used in EPI formulation could explain this difference. Our data support a neuroprotective effect of EPI which may provide neuroprotection in different neurodegenerative processes. The results highlight the role of CBD as the active component of EPI but also support the need for an appropriate formulation to dilute pharmaceutical cannabis-based productAgencia Nacional de Investigación e InnovaciónIBRO Neuroscience Reports2023-12-27T16:29:13Z2023-12-27T16:29:13Z2023-09-13Reporte técnicoinfo:eu-repo/semantics/reportPublicadoinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/20.500.12381/3368FCE_3_2020_1_162440IBRO Neuroscience Reports 15 (2023) S67–S466reponame:IIBCE en REDIinstname:Instituto de Investigaciones Biológicas Clemente Estableinstacron:Instituto de Investigaciones Biológicas Clemente EstableengAcceso abiertoinfo:eu-repo/semantics/openAccessDedicación de Dominio Público 1.0 Universal. (CC0)oai:redi.anii.org.uy:20.500.12381/33682026-06-16T05:25:31Z |
| spellingShingle | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures Echeverry, Carolina Cannabis sativa Epifractan XALEX Ciencias Médicas y de la Salud Medicina Básica Neurociencias |
| status_str | publishedVersion |
| title | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| title_full | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| title_fullStr | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| title_full_unstemmed | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| title_short | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| title_sort | Impact of different formulations of pharmaceutical cannabis-based extracts on the neuroprotective effect in cerebellar granule cell cultures |
| topic | Cannabis sativa Epifractan XALEX Ciencias Médicas y de la Salud Medicina Básica Neurociencias |
| url | https://hdl.handle.net/20.500.12381/3368 |