Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
Abstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livest...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890219826118656 |
|---|---|
| author | CASTELLS, M. |
| author2 | CAFFARENA, D. CASAUX, M.L. SCHILD, C. MIÑO, S. CASTELLS, F. CASTELLS, D. VICTORIA, M. RIET-CORREA, F. GIANNITTI, F. PAREÑO, V. COLINA, R. |
| author2_role | author author author author author author author author author author author |
| author_browse | CAFFARENA, D. CASAUX, M.L. CASTELLS, D. CASTELLS, F. CASTELLS, M. COLINA, R. GIANNITTI, F. MIÑO, S. PAREÑO, V. RIET-CORREA, F. SCHILD, C. VICTORIA, M. |
| author_facet | CASTELLS, M. CAFFARENA, D. CASAUX, M.L. SCHILD, C. MIÑO, S. CASTELLS, F. CASTELLS, D. VICTORIA, M. RIET-CORREA, F. GIANNITTI, F. PAREÑO, V. COLINA, R. |
| author_role | author |
| collection | AINFO |
| dc.creator.none.fl_str_mv | CASTELLS, M. CAFFARENA, D. CASAUX, M.L. SCHILD, C. MIÑO, S. CASTELLS, F. CASTELLS, D. VICTORIA, M. RIET-CORREA, F. GIANNITTI, F. PAREÑO, V. COLINA, R. |
| dc.date.none.fl_str_mv | 2020 2025-06-23T18:20:21Z 2025-06-23T18:20:21Z 2025-06-23T18:20:21Z |
| dc.identifier.none.fl_str_mv | https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250 |
| dc.language.none.fl_str_mv | en eng |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Acceso abierto |
| dc.source.none.fl_str_mv | reponame:AINFO instname:Instituto Nacional de Investigación Agropecuaria instacron:Instituto Nacional de Investigación Agropecuaria |
| dc.subject.none.fl_str_mv | PLATAFORMA SALUD ANIMAL ROTAVIRUS BOVINE GENOTYPES INTERSPECIES TRANSMISSION DIARRHEA GANADO BOVINO |
| dc.title.none.fl_str_mv | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| dc.type.none.fl_str_mv | Article PublishedVersion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Abstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_e67fef02ab585db747c6e41e577a024b |
| instacron_str | Instituto Nacional de Investigación Agropecuaria |
| institution | Instituto Nacional de Investigación Agropecuaria |
| instname_str | Instituto Nacional de Investigación Agropecuaria |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/4103 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| reponame_str | AINFO |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto |
| spelling | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.CASTELLS, M.CAFFARENA, D.CASAUX, M.L.SCHILD, C.MIÑO, S.CASTELLS, F.CASTELLS, D.VICTORIA, M.RIET-CORREA, F.GIANNITTI, F.PAREÑO, V.COLINA, R.PLATAFORMA SALUD ANIMALROTAVIRUSBOVINEGENOTYPESINTERSPECIES TRANSMISSIONDIARRHEAGANADO BOVINOAbstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.2025-06-23T18:20:21Z2025-06-23T18:20:21Z20202025-06-23T18:20:21ZArticlePublishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250reponame:AINFOinstname:Instituto Nacional de Investigación Agropecuariainstacron:Instituto Nacional de Investigación Agropecuariaenenginfo:eu-repo/semantics/openAccessAcceso abiertooai:redi.anii.org.uy:20.500.12381/41032026-02-10T17:37:51Z |
| spellingShingle | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. CASTELLS, M. PLATAFORMA SALUD ANIMAL ROTAVIRUS BOVINE GENOTYPES INTERSPECIES TRANSMISSION DIARRHEA GANADO BOVINO |
| status_str | publishedVersion |
| title | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| title_full | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| title_fullStr | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| title_full_unstemmed | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| title_short | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| title_sort | Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
| topic | PLATAFORMA SALUD ANIMAL ROTAVIRUS BOVINE GENOTYPES INTERSPECIES TRANSMISSION DIARRHEA GANADO BOVINO |
| url | https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250 |