Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis
from spermatogenic cells, is reflected at the transcriptional level, with the largest number of tissue-specific genes and long noncoding RNAs (lncRNAs) compared to other tissues, and one of the highest rates of alternative splicing. Although it is known that adequate alternative-splicing patterns an...
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| Language: | English |
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2024
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| Online Access: | https://hdl.handle.net/20.500.12381/5518 |
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| _version_ | 1868890219010326529 |
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| author | Romeo-Cardeillac, Carlos |
| author2 | Trovero, María Fernanda Radío, Santiago Smircich, Pablo Rodríguez-Casuriaga, Rosana Geisinger, Adriana Sotelo-Silveira José |
| author2_role | author author author author author author |
| author_browse | Geisinger, Adriana Radío, Santiago Rodríguez-Casuriaga, Rosana Romeo-Cardeillac, Carlos Smircich, Pablo Sotelo-Silveira José Trovero, María Fernanda |
| author_facet | Romeo-Cardeillac, Carlos Trovero, María Fernanda Radío, Santiago Smircich, Pablo Rodríguez-Casuriaga, Rosana Geisinger, Adriana Sotelo-Silveira José |
| author_role | author |
| collection | IIBCE en REDI |
| dc.creator.none.fl_str_mv | Romeo-Cardeillac, Carlos Trovero, María Fernanda Radío, Santiago Smircich, Pablo Rodríguez-Casuriaga, Rosana Geisinger, Adriana Sotelo-Silveira José |
| dc.date.none.fl_str_mv | 2024-03-20 2026-05-05T16:31:03Z 2026-05-05T16:31:03Z |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/5518 FCE_1_2021_1_166510 10.1186/s12864-024-10170-z |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | BMC |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Reconocimiento 4.0 Internacional. (CC BY) |
| dc.source.none.fl_str_mv | BMC Genomics reponame:IIBCE en REDI instname:Instituto de Investigaciones Biológicas Clemente Estable instacron:Instituto de Investigaciones Biológicas Clemente Estable |
| dc.subject.none.fl_str_mv | meiosis spermatogenesis transcriptomics alternative splicing lncRNAs Ciencias Naturales y Exactas Ciencias Biológicas Biología Reproductiva Bioquímica y Biología Molecular |
| dc.title.none.fl_str_mv | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | from spermatogenic cells, is reflected at the transcriptional level, with the largest number of tissue-specific genes and long noncoding RNAs (lncRNAs) compared to other tissues, and one of the highest rates of alternative splicing. Although it is known that adequate alternative-splicing patterns and stage-specific isoforms are critical for successful spermatogenesis, so far only a very limited number of reports have addressed a detailed study of alternative splicing and isoforms along the different spermatogenic stages. Results In the present work, using highly purified stage-specific testicular cell populations, we detected 33,002 transcripts expressed throughout mouse spermatogenesis not annotated so far. These include both splice variants of already annotated genes, and of hitherto unannotated genes. Using conservative criteria, we uncovered 13,471 spermatogenic lncRNAs, which reflects the still incomplete annotation of lncRNAs. A distinctive feature of lncRNAs was their lower number of splice variants compared to protein-coding ones, adding to the conclusion that lncRNAs are, in general, less complex than mRNAs. Besides, we identified 2,794 unannotated transcripts with high coding potential (including some arising from yet unannotated genes), many of which encode unnoticed putative testisspecific proteins. Some of the most interesting coding splice variants were chosen, and validated through RT-PCR. Remarkably, the largest number of stage-specific unannotated transcripts are expressed during early meiotic prophase stages, whose study has been scarcely addressed in former transcriptomic analyses. Conclusions We detected a high number of yet unannotated genes and alternatively spliced transcripts along mouse spermatogenesis, hence showing that the transcriptomic diversity of the testis is considerably higher than previously reported. This is especially prominent for specific, underrepresented stages such as those of early meiotic prophase, and its unveiling may constitute a step towards the understanding of their key events. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_e5dc1cc362ba51b3ab11b1ba2ca4d8e6 |
| identifier_str_mv | FCE_1_2021_1_166510 10.1186/s12864-024-10170-z |
| instacron_str | Instituto de Investigaciones Biológicas Clemente Estable |
| institution | Instituto de Investigaciones Biológicas Clemente Estable |
| instname_str | Instituto de Investigaciones Biológicas Clemente Estable |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/5518 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher.none.fl_str_mv | BMC |
| reponame_str | IIBCE en REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Reconocimiento 4.0 Internacional. (CC BY) |
| spelling | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesisRomeo-Cardeillac, CarlosTrovero, María FernandaRadío, SantiagoSmircich, PabloRodríguez-Casuriaga, RosanaGeisinger, AdrianaSotelo-Silveira Josémeiosisspermatogenesistranscriptomicsalternative splicinglncRNAsCiencias Naturales y ExactasCiencias BiológicasBiología ReproductivaBioquímica y Biología Molecularfrom spermatogenic cells, is reflected at the transcriptional level, with the largest number of tissue-specific genes and long noncoding RNAs (lncRNAs) compared to other tissues, and one of the highest rates of alternative splicing. Although it is known that adequate alternative-splicing patterns and stage-specific isoforms are critical for successful spermatogenesis, so far only a very limited number of reports have addressed a detailed study of alternative splicing and isoforms along the different spermatogenic stages. Results In the present work, using highly purified stage-specific testicular cell populations, we detected 33,002 transcripts expressed throughout mouse spermatogenesis not annotated so far. These include both splice variants of already annotated genes, and of hitherto unannotated genes. Using conservative criteria, we uncovered 13,471 spermatogenic lncRNAs, which reflects the still incomplete annotation of lncRNAs. A distinctive feature of lncRNAs was their lower number of splice variants compared to protein-coding ones, adding to the conclusion that lncRNAs are, in general, less complex than mRNAs. Besides, we identified 2,794 unannotated transcripts with high coding potential (including some arising from yet unannotated genes), many of which encode unnoticed putative testisspecific proteins. Some of the most interesting coding splice variants were chosen, and validated through RT-PCR. Remarkably, the largest number of stage-specific unannotated transcripts are expressed during early meiotic prophase stages, whose study has been scarcely addressed in former transcriptomic analyses. Conclusions We detected a high number of yet unannotated genes and alternatively spliced transcripts along mouse spermatogenesis, hence showing that the transcriptomic diversity of the testis is considerably higher than previously reported. This is especially prominent for specific, underrepresented stages such as those of early meiotic prophase, and its unveiling may constitute a step towards the understanding of their key events.Agencia Nacional de Investigación e InnovaciónComisión Sectorial Investigación Científica (CSIC, UdelaR)BMC2026-05-05T16:31:03Z2026-05-05T16:31:03Z2024-03-20Artículoinfo:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/20.500.12381/5518FCE_1_2021_1_16651010.1186/s12864-024-10170-zBMC Genomicsreponame:IIBCE en REDIinstname:Instituto de Investigaciones Biológicas Clemente Estableinstacron:Instituto de Investigaciones Biológicas Clemente EstableengAcceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento 4.0 Internacional. (CC BY)oai:redi.anii.org.uy:20.500.12381/55182026-06-16T05:25:31Z |
| spellingShingle | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis Romeo-Cardeillac, Carlos meiosis spermatogenesis transcriptomics alternative splicing lncRNAs Ciencias Naturales y Exactas Ciencias Biológicas Biología Reproductiva Bioquímica y Biología Molecular |
| status_str | publishedVersion |
| title | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| title_full | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| title_fullStr | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| title_full_unstemmed | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| title_short | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| title_sort | Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis |
| topic | meiosis spermatogenesis transcriptomics alternative splicing lncRNAs Ciencias Naturales y Exactas Ciencias Biológicas Biología Reproductiva Bioquímica y Biología Molecular |
| url | https://hdl.handle.net/20.500.12381/5518 |