Sex steroid modulation of cortisol secretion in sheep
There is strong evidence that the gonads modulate the hypothalamic–pituitary–adrenal axis. To investigate these sex differences at the adrenal glands of sheep we compared the cortisol response to ACTH (experiment 1) and measured the relative expression of oestrogen receptor alpha (ERS1), androgen re...
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| Format: | article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/20.500.12008/50640 https://doi.org/10.1017/S1751731114000780 |
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| _version_ | 1868890213342773248 |
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| author | Van Lier, Elize |
| author2 | Carriquiry Fossemale, Mariana Meikle, Ana |
| author2_role | author author |
| author_browse | Carriquiry Fossemale, Mariana Meikle, Ana Van Lier, Elize |
| author_facet | Van Lier, Elize Carriquiry Fossemale, Mariana Meikle, Ana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Van Lier Elize, Universidad de la República (Uruguay). Facultad de Agronomía. Departamento de Producción Animal y Pasturas Carriquiry Fossemale Mariana, Universidad de la República (Uruguay). Facultad de Agronomía. Departamento de Producción Animal y Pasturas ; Universidad de la República (Uruguay). Facultad de Agronomía. Estación Experimental Meikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Técnicas Nucleares |
| dc.creator.none.fl_str_mv | Van Lier, Elize Carriquiry Fossemale, Mariana Meikle, Ana |
| dc.date.none.fl_str_mv | 2014 2025-07-16T12:06:20Z 2025-07-16T12:06:20Z |
| dc.format.none.fl_str_mv | 8 p application/pdf |
| dc.identifier.none.fl_str_mv | Van Lier, E, Carriquiry Fossemale, M y Meikle, A. Sex steroid modulation of cortisol secretion in sheep. Animal. [en línea] 2014, 8(6), 960-967 https://hdl.handle.net/20.500.12008/50640 https://doi.org/10.1017/S1751731114000780 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Animal, 2014, 8(6), 960-967 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | OVINOS OVEJAS CARNEROS ESTEROIDES GLANDULAS SUPRARRENALES |
| dc.title.none.fl_str_mv | Sex steroid modulation of cortisol secretion in sheep |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | There is strong evidence that the gonads modulate the hypothalamic–pituitary–adrenal axis. To investigate these sex differences at the adrenal glands of sheep we compared the cortisol response to ACTH (experiment 1) and measured the relative expression of oestrogen receptor alpha (ERS1), androgen receptor (AR), melanocortin 2 receptor (MC2R) and steroid acute regulatory protein (STAR) mRNA in adrenal glands (experiment 2) of gonadectomised rams and ewes either with or without sex steroid replacement. In experiment 1 six castrated adult rams and four ovariectomised adult ewes were used in two ACTH trials. On each trial blood samples were taken every 15 min for 4 h through an indwelling jugular catheter and each animal received 0.5 mg of an ACTH analogue i.v., immediately after the sample at 1 h from the beginning of the trial. Four days after the first trial the males received 100 mg of Testosterone Cyclopentilpropionate (TC) i.m. and the females received 2.5 mg of Oestradiol Benzoate (EB) i.m. At 72 h after TC or EB administration the second trial was performed. In experiment 2 the adrenal glands were obtained from gonadectomised adult rams (n = 8) and adult ewes (n = 8). Four rams received 100 mg of TC i.m. and four females received 0.5 mg of EB i.m. Blood samples were taken at 0, 12, 24, 48 and 72 h relative to steroid replacement and the animals were thereafter slaughtered. Cortisol, testosterone and 17β-oestradiol were determined by radioimmunoanalysis. The transcripts of ERS1, AR, MC2R and STAR were determined by real-time reverse transcription PCR in adrenal tissue. Cortisol secretion was higher in female sheep than in male sheep, and higher in EB-treated than non-treated ewes. No difference in cortisol secretion was observed between TC-treated and non-treated rams. Gonadectomised rams treated with TC presented greater AR mRNA and MC2R mRNA expression than males without the steroid replacement. Gonadectomised ewes treated with EB tended to present lower AR mRNA than the ones without steroid replacement. Gonadectomised rams with TC also had greater AR mRNA, ERS1 mRNA and MC2R mRNA expression than ewes treated with EB. The relative amount of STAR transcript was not different among the different groups. The results confirm sex differences in ACTH-induced cortisol secretion in sheep, as well as in the expression of the receptor proteins for both 17β-oestradiol and testosterone in the sheep adrenal gland. However, the underlying mechanisms for sex steroid modulation remain unresolved. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_e058d7b9f65f5dbd46d1f49ce477ae7e |
| identifier_str_mv | Van Lier, E, Carriquiry Fossemale, M y Meikle, A. Sex steroid modulation of cortisol secretion in sheep. Animal. [en línea] 2014, 8(6), 960-967 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50640 |
| publishDate | 2014 |
| publishDateSort | 2014 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Sex steroid modulation of cortisol secretion in sheepVan Lier, ElizeCarriquiry Fossemale, MarianaMeikle, AnaOVINOSOVEJASCARNEROSESTEROIDESGLANDULAS SUPRARRENALESThere is strong evidence that the gonads modulate the hypothalamic–pituitary–adrenal axis. To investigate these sex differences at the adrenal glands of sheep we compared the cortisol response to ACTH (experiment 1) and measured the relative expression of oestrogen receptor alpha (ERS1), androgen receptor (AR), melanocortin 2 receptor (MC2R) and steroid acute regulatory protein (STAR) mRNA in adrenal glands (experiment 2) of gonadectomised rams and ewes either with or without sex steroid replacement. In experiment 1 six castrated adult rams and four ovariectomised adult ewes were used in two ACTH trials. On each trial blood samples were taken every 15 min for 4 h through an indwelling jugular catheter and each animal received 0.5 mg of an ACTH analogue i.v., immediately after the sample at 1 h from the beginning of the trial. Four days after the first trial the males received 100 mg of Testosterone Cyclopentilpropionate (TC) i.m. and the females received 2.5 mg of Oestradiol Benzoate (EB) i.m. At 72 h after TC or EB administration the second trial was performed. In experiment 2 the adrenal glands were obtained from gonadectomised adult rams (n = 8) and adult ewes (n = 8). Four rams received 100 mg of TC i.m. and four females received 0.5 mg of EB i.m. Blood samples were taken at 0, 12, 24, 48 and 72 h relative to steroid replacement and the animals were thereafter slaughtered. Cortisol, testosterone and 17β-oestradiol were determined by radioimmunoanalysis. The transcripts of ERS1, AR, MC2R and STAR were determined by real-time reverse transcription PCR in adrenal tissue. Cortisol secretion was higher in female sheep than in male sheep, and higher in EB-treated than non-treated ewes. No difference in cortisol secretion was observed between TC-treated and non-treated rams. Gonadectomised rams treated with TC presented greater AR mRNA and MC2R mRNA expression than males without the steroid replacement. Gonadectomised ewes treated with EB tended to present lower AR mRNA than the ones without steroid replacement. Gonadectomised rams with TC also had greater AR mRNA, ERS1 mRNA and MC2R mRNA expression than ewes treated with EB. The relative amount of STAR transcript was not different among the different groups. The results confirm sex differences in ACTH-induced cortisol secretion in sheep, as well as in the expression of the receptor proteins for both 17β-oestradiol and testosterone in the sheep adrenal gland. However, the underlying mechanisms for sex steroid modulation remain unresolved.Van Lier Elize, Universidad de la República (Uruguay). Facultad de Agronomía. Departamento de Producción Animal y PasturasCarriquiry Fossemale Mariana, Universidad de la República (Uruguay). Facultad de Agronomía. Departamento de Producción Animal y Pasturas ; Universidad de la República (Uruguay). Facultad de Agronomía. Estación ExperimentalMeikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Técnicas Nucleares2025-07-16T12:06:20Z2025-07-16T12:06:20Z2014Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 papplication/pdfVan Lier, E, Carriquiry Fossemale, M y Meikle, A. Sex steroid modulation of cortisol secretion in sheep. Animal. [en línea] 2014, 8(6), 960-967https://hdl.handle.net/20.500.12008/50640https://doi.org/10.1017/S1751731114000780reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengAnimal, 2014, 8(6), 960-967Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/506402026-04-14T10:35:07Z |
| spellingShingle | Sex steroid modulation of cortisol secretion in sheep Van Lier, Elize OVINOS OVEJAS CARNEROS ESTEROIDES GLANDULAS SUPRARRENALES |
| status_str | publishedVersion |
| title | Sex steroid modulation of cortisol secretion in sheep |
| title_full | Sex steroid modulation of cortisol secretion in sheep |
| title_fullStr | Sex steroid modulation of cortisol secretion in sheep |
| title_full_unstemmed | Sex steroid modulation of cortisol secretion in sheep |
| title_short | Sex steroid modulation of cortisol secretion in sheep |
| title_sort | Sex steroid modulation of cortisol secretion in sheep |
| topic | OVINOS OVEJAS CARNEROS ESTEROIDES GLANDULAS SUPRARRENALES |
| url | https://hdl.handle.net/20.500.12008/50640 https://doi.org/10.1017/S1751731114000780 |