Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways
Signal-regulatory protein alpha (SIRPA) is a well-known inhibitor of phagocytosis when it complexes with CD47 expressed on target cells. Here we show that SIRPA decreased in vitro infection by a number of pathogenic viruses, including New World and Old World arena-viruses, Zika virus, vesicular stom...
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| Eará dahkkit: | , , , , , |
| Materiálatiipa: | article |
| Giella: | eaŋgalasgiella |
| Almmustuhtton: |
2021
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| Fáttát: | |
| Liŋkkat: | https://hdl.handle.net/20.500.12008/38247 |
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| _version_ | 1868890207186583552 |
|---|---|
| author | Sarute, Nicolás |
| author2 | Cheng, Han Yan, Zhonghao Salas-Briceno, Karen Richne, Justin Rong, Lijun Ross, S. R. |
| author2_role | author author author author author author |
| author_browse | Cheng, Han Richne, Justin Rong, Lijun Ross, S. R. Salas-Briceno, Karen Sarute, Nicolás Yan, Zhonghao |
| author_facet | Sarute, Nicolás Cheng, Han Yan, Zhonghao Salas-Briceno, Karen Richne, Justin Rong, Lijun Ross, S. R. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Sarute Nicolás, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Cheng Han, University of Illinois Yan Zhonghao, University of Illinois Salas-Briceno Karen, University of Illinois Richne Justin, University of Illinois Rong Lijun, University of Illinois Ross S. R., University of Illinois |
| dc.creator.none.fl_str_mv | Sarute, Nicolás Cheng, Han Yan, Zhonghao Salas-Briceno, Karen Richne, Justin Rong, Lijun Ross, S. R. |
| dc.date.none.fl_str_mv | 2021 2023-07-20T15:08:04Z 2023-07-20T15:08:04Z |
| dc.format.none.fl_str_mv | 23 h application/pdf |
| dc.identifier.none.fl_str_mv | Sarute, N, Cheng, H, Yan, Z, [y otros autores] "Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways". PLoS Pathog. [en línea] 2021, 17(6): e1009662. 23 h. DOI: 10.1371/journal.ppat.1009662 1553-7374 https://hdl.handle.net/20.500.12008/38247 10.1371/journal.ppat.1009662 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | PloS ONE |
| dc.relation.none.fl_str_mv | PLoS Pathog,2021, 17(6): e1009662. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Vesicular stomatitis virus Ebola virus Small interfering RNA Phagocytosis SARS CoV 2 293T cells Transfection Machupo Virus |
| dc.title.none.fl_str_mv | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Signal-regulatory protein alpha (SIRPA) is a well-known inhibitor of phagocytosis when it complexes with CD47 expressed on target cells. Here we show that SIRPA decreased in vitro infection by a number of pathogenic viruses, including New World and Old World arena-viruses, Zika virus, vesicular stomatitis virus and pseudoviruses bearing the Machupo virus, Ebola virus and SARS-CoV-2 glycoproteins, but not HSV-1, MLV or mNoV. Moreover, mice with targeted mutation of the Sirpa gene that renders it non-functional were more suscepti ble to infection with the New World arenaviruses Junı´n virus vaccine strain Candid 1 and Tacaribe virus, but not MLV or mNoV. All SIRPA-inhibited viruses have in common the requirement for trafficking to a low pH endosomal compartment. This was clearly demonstrated with SARS-CoV-2 pseudovirus, which was only inhibited by SIRPA in cells in which it required trafficking to the endosome. Similar to its role in phagocytosis inhibition, SIRPA decreased virus internalization but not binding to cell surface receptors. We also found that increasing SIRPA levels via treatment with IL-4 led to even greater anti-viral activity. These data suggest that enhancing SIRPA’s activity could be a target for anti-viral therapies. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_da85067a3da7fa3fef723cf6dfd5b3de |
| identifier_str_mv | Sarute, N, Cheng, H, Yan, Z, [y otros autores] "Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways". PLoS Pathog. [en línea] 2021, 17(6): e1009662. 23 h. DOI: 10.1371/journal.ppat.1009662 1553-7374 10.1371/journal.ppat.1009662 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/38247 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher.none.fl_str_mv | PloS ONE |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathwaysSarute, NicolásCheng, HanYan, ZhonghaoSalas-Briceno, KarenRichne, JustinRong, LijunRoss, S. R.Vesicular stomatitis virusEbola virusSmall interfering RNAPhagocytosisSARS CoV 2293T cellsTransfectionMachupo VirusSignal-regulatory protein alpha (SIRPA) is a well-known inhibitor of phagocytosis when it complexes with CD47 expressed on target cells. Here we show that SIRPA decreased in vitro infection by a number of pathogenic viruses, including New World and Old World arena-viruses, Zika virus, vesicular stomatitis virus and pseudoviruses bearing the Machupo virus, Ebola virus and SARS-CoV-2 glycoproteins, but not HSV-1, MLV or mNoV. Moreover, mice with targeted mutation of the Sirpa gene that renders it non-functional were more suscepti ble to infection with the New World arenaviruses Junı´n virus vaccine strain Candid 1 and Tacaribe virus, but not MLV or mNoV. All SIRPA-inhibited viruses have in common the requirement for trafficking to a low pH endosomal compartment. This was clearly demonstrated with SARS-CoV-2 pseudovirus, which was only inhibited by SIRPA in cells in which it required trafficking to the endosome. Similar to its role in phagocytosis inhibition, SIRPA decreased virus internalization but not binding to cell surface receptors. We also found that increasing SIRPA levels via treatment with IL-4 led to even greater anti-viral activity. These data suggest that enhancing SIRPA’s activity could be a target for anti-viral therapies.PloS ONESarute Nicolás, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Cheng Han, University of IllinoisYan Zhonghao, University of IllinoisSalas-Briceno Karen, University of IllinoisRichne Justin, University of IllinoisRong Lijun, University of IllinoisRoss S. R., University of Illinois2023-07-20T15:08:04Z2023-07-20T15:08:04Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion23 happlication/pdfSarute, N, Cheng, H, Yan, Z, [y otros autores] "Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways". PLoS Pathog. [en línea] 2021, 17(6): e1009662. 23 h. DOI: 10.1371/journal.ppat.10096621553-7374https://hdl.handle.net/20.500.12008/3824710.1371/journal.ppat.1009662reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengPLoS Pathog,2021, 17(6): e1009662.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/382472026-04-14T10:10:07Z |
| spellingShingle | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways Sarute, Nicolás Vesicular stomatitis virus Ebola virus Small interfering RNA Phagocytosis SARS CoV 2 293T cells Transfection Machupo Virus |
| status_str | publishedVersion |
| title | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| title_full | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| title_fullStr | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| title_full_unstemmed | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| title_short | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| title_sort | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways |
| topic | Vesicular stomatitis virus Ebola virus Small interfering RNA Phagocytosis SARS CoV 2 293T cells Transfection Machupo Virus |
| url | https://hdl.handle.net/20.500.12008/38247 |