RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi
Trypanosomes regulate gene expression mainly by using posttranscriptional mechanisms. Key factors responsible for carrying out this regulation are RNA-binding proteins, affecting subcellular localization, translation, and/or transcript stability. Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP...
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| Ētahi atu kaituhi: | , , |
| Hōputu: | article |
| Reo: | Ingarihi |
| I whakaputaina: |
2023
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| Ngā marau: | |
| Urunga tuihono: | https://hdl.handle.net/20.500.12008/43168 |
| Ngā Tūtohu: |
Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
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| _version_ | 1868890207039782912 |
|---|---|
| author | Sabalette, Karina B |
| author2 | Sotelo-Silveira, José Roberto Smircich, Pablo De Gaudenzi, Javier G. |
| author2_role | author author author |
| author_browse | De Gaudenzi, Javier G. Sabalette, Karina B Smircich, Pablo Sotelo-Silveira, José Roberto |
| author_facet | Sabalette, Karina B Sotelo-Silveira, José Roberto Smircich, Pablo De Gaudenzi, Javier G. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Sabalette Karina B Sotelo-Silveira José Roberto, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. De Gaudenzi Javier G. |
| dc.creator.none.fl_str_mv | Sabalette, Karina B Sotelo-Silveira, José Roberto Smircich, Pablo De Gaudenzi, Javier G. |
| dc.date.none.fl_str_mv | 2023 2024-03-19T12:14:40Z 2024-03-19T12:14:40Z |
| dc.format.none.fl_str_mv | 15 h. application/pdf |
| dc.identifier.none.fl_str_mv | Sabalette, K, Sotelo-Silveira, J, Smircich, P [y otros autores]. "RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi". Journal of Biological Chemistry. [en línea] 2023, 299(5): 104623. 15 h. DOI: 10.1016/j.jbc.2023.104623. 0021-9258 https://hdl.handle.net/20.500.12008/43168 10.1016/j.jbc.2023.104623 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | ASBMB |
| dc.relation.none.fl_str_mv | Journal of Biological Chemistry, 2023, 299(5): 104623. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Trypanosome RNA–protein interaction RNA-binding protein Gene regulation RNA regulon |
| dc.title.none.fl_str_mv | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Trypanosomes regulate gene expression mainly by using posttranscriptional mechanisms. Key factors responsible for carrying out this regulation are RNA-binding proteins, affecting subcellular localization, translation, and/or transcript stability. Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) is a small protein that modulates the expression of several surface glycoproteins of the trypomastigote infective stage of the parasite. Its mRNA targets are known, but the impact of its overexpression at the transcriptome level in the insect-dwelling epimastigote cells has not yet been investigated. Thus, in the present study, by using a tetracycline-inducible system, we generated a population of TcUBP1-overexpressing parasites and analyzed its effect by RNA-Seq methodology. This allowed us to identify 793 up- and 371 downregulated genes with respect to the wildtype control sample. Among the upregulated genes, it was possible to identify members coding for the TcS superfamily, MASP, MUCI/II, and protein kinases, whereas among the downregulated transcripts, we found mainly genes coding for ribosomal, mitochondrial, and synthetic pathway proteins. RNA-Seq comparison with two previously published datasets revealed that the expression profile of this TcUBP1- overexpressing replicative epimastigote form resembles the transition to the infective metacyclic trypomastigote stage. We identified novel cis-regulatory elements in the 30 -untranslated region of the affected transcripts and confirmed that UBP1m, a signature TcUBP1 binding element previously characterized in our laboratory, is enriched in the list of stabilized genes. We can conclude that the overall effect of TcUBP1 overexpression on the epimastigote transcriptome is mainly the stabilization of mRNAs coding for proteins that are important for parasite infection. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_da1fa39fe5f798719deae733bf612cff |
| identifier_str_mv | Sabalette, K, Sotelo-Silveira, J, Smircich, P [y otros autores]. "RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi". Journal of Biological Chemistry. [en línea] 2023, 299(5): 104623. 15 h. DOI: 10.1016/j.jbc.2023.104623. 0021-9258 10.1016/j.jbc.2023.104623 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/43168 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | ASBMB |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruziSabalette, Karina BSotelo-Silveira, José RobertoSmircich, PabloDe Gaudenzi, Javier G.TrypanosomeRNA–protein interactionRNA-binding proteinGene regulationRNA regulonTrypanosomes regulate gene expression mainly by using posttranscriptional mechanisms. Key factors responsible for carrying out this regulation are RNA-binding proteins, affecting subcellular localization, translation, and/or transcript stability. Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) is a small protein that modulates the expression of several surface glycoproteins of the trypomastigote infective stage of the parasite. Its mRNA targets are known, but the impact of its overexpression at the transcriptome level in the insect-dwelling epimastigote cells has not yet been investigated. Thus, in the present study, by using a tetracycline-inducible system, we generated a population of TcUBP1-overexpressing parasites and analyzed its effect by RNA-Seq methodology. This allowed us to identify 793 up- and 371 downregulated genes with respect to the wildtype control sample. Among the upregulated genes, it was possible to identify members coding for the TcS superfamily, MASP, MUCI/II, and protein kinases, whereas among the downregulated transcripts, we found mainly genes coding for ribosomal, mitochondrial, and synthetic pathway proteins. RNA-Seq comparison with two previously published datasets revealed that the expression profile of this TcUBP1- overexpressing replicative epimastigote form resembles the transition to the infective metacyclic trypomastigote stage. We identified novel cis-regulatory elements in the 30 -untranslated region of the affected transcripts and confirmed that UBP1m, a signature TcUBP1 binding element previously characterized in our laboratory, is enriched in the list of stabilized genes. We can conclude that the overall effect of TcUBP1 overexpression on the epimastigote transcriptome is mainly the stabilization of mRNAs coding for proteins that are important for parasite infection.ASBMBSabalette Karina BSotelo-Silveira José Roberto, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.De Gaudenzi Javier G.2024-03-19T12:14:40Z2024-03-19T12:14:40Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 h.application/pdfSabalette, K, Sotelo-Silveira, J, Smircich, P [y otros autores]. "RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi". Journal of Biological Chemistry. [en línea] 2023, 299(5): 104623. 15 h. DOI: 10.1016/j.jbc.2023.104623.0021-9258https://hdl.handle.net/20.500.12008/4316810.1016/j.jbc.2023.104623reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Biological Chemistry, 2023, 299(5): 104623.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/431682026-04-14T10:10:34Z |
| spellingShingle | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi Sabalette, Karina B Trypanosome RNA–protein interaction RNA-binding protein Gene regulation RNA regulon |
| status_str | publishedVersion |
| title | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| title_full | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| title_fullStr | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| title_full_unstemmed | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| title_short | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| title_sort | RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi |
| topic | Trypanosome RNA–protein interaction RNA-binding protein Gene regulation RNA regulon |
| url | https://hdl.handle.net/20.500.12008/43168 |