Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42
Flavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5 to 6.5 a...
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| Other Authors: | , , |
| Format: | article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/20.500.12008/27228 |
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| _version_ | 1868890206194630656 |
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| author | Herrera, Lorena |
| author2 | Braña, Victoria Franco-Fraguas, Laura Castro-Sowinski, Susana |
| author2_role | author author author |
| author_browse | Braña, Victoria Castro-Sowinski, Susana Franco-Fraguas, Laura Herrera, Lorena |
| author_facet | Herrera, Lorena Braña, Victoria Franco-Fraguas, Laura Castro-Sowinski, Susana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Herrera Lorena, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Braña Victoria, IIBCE Franco-Fraguas Laura, Universidad de la República (Uruguay). Facultad de Química. Castro-Sowinski Susana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. |
| dc.creator.none.fl_str_mv | Herrera, Lorena Braña, Victoria Franco-Fraguas, Laura Castro-Sowinski, Susana |
| dc.date.none.fl_str_mv | 2019 2021-04-23T14:25:28Z 2021-04-23T14:25:28Z |
| dc.format.none.fl_str_mv | 9 h. application/pdf |
| dc.identifier.none.fl_str_mv | Herrera, L, Braña, V, Franco-Fraguas, L. y otros. "Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42". Microbiological Research. [en línea] 2019, 223-225:13-23. 9 h. DOI: 10.1016/j.micres.2019.03.009 0944-5013 https://hdl.handle.net/20.500.12008/27228 10.1016/j.micres.2019.03.009 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Urban and Fischer |
| dc.relation.none.fl_str_mv | Microbiological Research, 2019, 223-225: 13-23 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Antarctica Carbon cycle Cellulase Flavobacterium Saccharification |
| dc.title.none.fl_str_mv | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Flavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5 to 6.5 and 40 to 50 °C, respectively). The time-course analyses of the bacterial growth and cellulase production showed that the cocktail has maximal activity at the stationary phase when growing at 16 °C with filter paper as a cellulosic carbon source, among the tested substrates. The analyses of the CAZome and the identification of secreted proteins by shotgun Mass Spectrometry analysis showed that five glycoside hydrolyses are present in the bacterial secretome, which probably cooperate in the degradation of the cellulosic substrates. Two of these glycoside hydrolyses may harbor putative carbohydrate binding modules, both with a cleft-like active site. The cellulolytic cocktail was assayed in saccharification experiments using carboxymethylcellulose as a substrate and results showed the release of glucose (a fermentable sugar) and other reducing-sugars, after 24 h incubation. The ecological relevance of producing cellulases in the Antarctic environment, as well as their potential use in the bio-refinery industry, are discussed. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_d954204b7124e34ef292d00a7c98a3a2 |
| identifier_str_mv | Herrera, L, Braña, V, Franco-Fraguas, L. y otros. "Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42". Microbiological Research. [en línea] 2019, 223-225:13-23. 9 h. DOI: 10.1016/j.micres.2019.03.009 0944-5013 10.1016/j.micres.2019.03.009 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/27228 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Urban and Fischer |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42Herrera, LorenaBraña, VictoriaFranco-Fraguas, LauraCastro-Sowinski, SusanaAntarcticaCarbon cycleCellulaseFlavobacteriumSaccharificationFlavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5 to 6.5 and 40 to 50 °C, respectively). The time-course analyses of the bacterial growth and cellulase production showed that the cocktail has maximal activity at the stationary phase when growing at 16 °C with filter paper as a cellulosic carbon source, among the tested substrates. The analyses of the CAZome and the identification of secreted proteins by shotgun Mass Spectrometry analysis showed that five glycoside hydrolyses are present in the bacterial secretome, which probably cooperate in the degradation of the cellulosic substrates. Two of these glycoside hydrolyses may harbor putative carbohydrate binding modules, both with a cleft-like active site. The cellulolytic cocktail was assayed in saccharification experiments using carboxymethylcellulose as a substrate and results showed the release of glucose (a fermentable sugar) and other reducing-sugars, after 24 h incubation. The ecological relevance of producing cellulases in the Antarctic environment, as well as their potential use in the bio-refinery industry, are discussed.Urban and FischerHerrera Lorena, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Braña Victoria, IIBCEFranco-Fraguas Laura, Universidad de la República (Uruguay). Facultad de Química.Castro-Sowinski Susana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.2021-04-23T14:25:28Z2021-04-23T14:25:28Z2019Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 h.application/pdfHerrera, L, Braña, V, Franco-Fraguas, L. y otros. "Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42". Microbiological Research. [en línea] 2019, 223-225:13-23. 9 h. DOI: 10.1016/j.micres.2019.03.0090944-5013https://hdl.handle.net/20.500.12008/2722810.1016/j.micres.2019.03.009reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengMicrobiological Research, 2019, 223-225: 13-23Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/272282026-04-14T10:09:36Z |
| spellingShingle | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 Herrera, Lorena Antarctica Carbon cycle Cellulase Flavobacterium Saccharification |
| status_str | publishedVersion |
| title | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| title_full | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| title_fullStr | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| title_full_unstemmed | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| title_short | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| title_sort | Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42 |
| topic | Antarctica Carbon cycle Cellulase Flavobacterium Saccharification |
| url | https://hdl.handle.net/20.500.12008/27228 |