Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas
Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods...
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| Formato: | article |
| Idioma: | inglés |
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2016
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| Acceso en liña: | https://hdl.handle.net/20.500.12008/31444 |
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| _version_ | 1868890204935290880 |
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| author | González González, Rogelio |
| author2 | Molina-Frechero, Nelly Carreón Burciaga, Ramón Gil Robles Bonilla, Carlos Pereira-Prado, Vanesa Bologna-Molina, Ronell |
| author2_role | author author author author author |
| author_browse | Bologna-Molina, Ronell Carreón Burciaga, Ramón Gil González González, Rogelio Molina-Frechero, Nelly Pereira-Prado, Vanesa Robles Bonilla, Carlos |
| author_facet | González González, Rogelio Molina-Frechero, Nelly Carreón Burciaga, Ramón Gil Robles Bonilla, Carlos Pereira-Prado, Vanesa Bologna-Molina, Ronell |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | González González Rogelio, Department of Research, School of Dentistry, Universidad Ju´arez del Estado de Durango, Durango, DGO, Mexico Molina Frechero Nelly, 2Department of Health Care, Universidad Autónoma Metropolitana, Unidad Xochimilco,Mexico City, Mexico Carreón Burciaga Ramon Gil, Department of Research, School of Dentistry, Universidad Ju´arez del Estado de Durango, Durango, DGO, Mexico López Verdínnell Bologna-Molina4 Sandra5, Microbiology and Pathology Department, Dentistry Research Institute, Health Sciences University Center, Universidad de Guadalajara, Guadalajara, JAL, Mexico Robles Bonilla Carlos, 4School of Health Sciences, Universidad Anahuac Norte, Lomas An´ahuac, Huixquilucan, MEX, Mexico Pereira-Prado Vanesa, Department of Molecular Pathology, School of Dentistry, Universidad de la Rep´ublica, Uruguay, Montevideo, Uruguay Bologna-Molina Ronell, School of Health Sciences, Universidad Anahuac Norte, Lomas An´ahuac, Huixquilucan, MEX, Mexico 5Department of Molecular Pathology, School of Dentistry, Universidad de la Rep´ublica, Uruguay, Montevideo, Uruguay |
| dc.creator.none.fl_str_mv | González González, Rogelio Molina-Frechero, Nelly Carreón Burciaga, Ramón Gil Robles Bonilla, Carlos Pereira-Prado, Vanesa Bologna-Molina, Ronell |
| dc.date.none.fl_str_mv | 2016 2022-05-05T15:25:55Z 2022-05-05T15:25:55Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | González González, R, Molina-Frechero, N, Carreón Burciaga, R. "Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas"Analytical Cellular Pathology Volume 2016 [en línea] 2016. dx.doi.org/10.1155/2016/7486989 https://hdl.handle.net/20.500.12008/31444 dx.doi.org/10.1155/2016/7486989 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Analytical Cellular Pathology Volume 2016 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | AMELOBLASTOMA RECUENTO DE CELULAS ANTIGENO Ki-67 AUTOMATIZACION COLORACION Y ETIQUETADO |
| dc.title.none.fl_str_mv | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose.The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of thesemethods.The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results.Therefore, this automated cell counting method must be supplemented with manual cell counting methods. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_d7b8a28969fecd0df7b0adebfa1cec68 |
| identifier_str_mv | González González, R, Molina-Frechero, N, Carreón Burciaga, R. "Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas"Analytical Cellular Pathology Volume 2016 [en línea] 2016. dx.doi.org/10.1155/2016/7486989 dx.doi.org/10.1155/2016/7486989 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31444 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| spelling | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in AmeloblastomasGonzález González, RogelioMolina-Frechero, NellyCarreón Burciaga, Ramón GilRobles Bonilla, CarlosPereira-Prado, VanesaBologna-Molina, RonellAMELOBLASTOMARECUENTO DE CELULASANTIGENO Ki-67AUTOMATIZACIONCOLORACION Y ETIQUETADOAmeloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose.The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of thesemethods.The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results.Therefore, this automated cell counting method must be supplemented with manual cell counting methods.González González Rogelio, Department of Research, School of Dentistry, Universidad Ju´arez del Estado de Durango, Durango, DGO, MexicoMolina Frechero Nelly, 2Department of Health Care, Universidad Autónoma Metropolitana, Unidad Xochimilco,Mexico City, MexicoCarreón Burciaga Ramon Gil, Department of Research, School of Dentistry, Universidad Ju´arez del Estado de Durango, Durango, DGO, MexicoLópez Verdínnell Bologna-Molina4 Sandra5, Microbiology and Pathology Department, Dentistry Research Institute, Health Sciences University Center, Universidad de Guadalajara, Guadalajara, JAL, MexicoRobles Bonilla Carlos, 4School of Health Sciences, Universidad Anahuac Norte, Lomas An´ahuac, Huixquilucan, MEX, MexicoPereira-Prado Vanesa, Department of Molecular Pathology, School of Dentistry, Universidad de la Rep´ublica, Uruguay, Montevideo, UruguayBologna-Molina Ronell, School of Health Sciences, Universidad Anahuac Norte, Lomas An´ahuac, Huixquilucan, MEX, Mexico 5Department of Molecular Pathology, School of Dentistry, Universidad de la Rep´ublica, Uruguay, Montevideo, Uruguay2022-05-05T15:25:55Z2022-05-05T15:25:55Z2016Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfGonzález González, R, Molina-Frechero, N, Carreón Burciaga, R. "Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas"Analytical Cellular Pathology Volume 2016 [en línea] 2016. dx.doi.org/10.1155/2016/7486989https://hdl.handle.net/20.500.12008/31444dx.doi.org/10.1155/2016/7486989reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengAnalytical Cellular Pathology Volume 2016Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)oai:colibri.udelar.edu.uy:20.500.12008/314442026-04-14T10:16:31Z |
| spellingShingle | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas González González, Rogelio AMELOBLASTOMA RECUENTO DE CELULAS ANTIGENO Ki-67 AUTOMATIZACION COLORACION Y ETIQUETADO |
| status_str | publishedVersion |
| title | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| title_full | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| title_fullStr | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| title_full_unstemmed | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| title_short | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| title_sort | Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas |
| topic | AMELOBLASTOMA RECUENTO DE CELULAS ANTIGENO Ki-67 AUTOMATIZACION COLORACION Y ETIQUETADO |
| url | https://hdl.handle.net/20.500.12008/31444 |