Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues

PIWI-interacting RNAs (piRNAs) are regarded as the guardians of the genome because theytackle genome stability-threatening transposable elements in the germline. Recently, piRNAswere also reported in other types of cells, including mouse brain, malignant and non-malignant somatic tissues, and human...

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Hlavní autor: Tosar Rovira, Juan Pablo (author)
Další autoři: Rovira, Carlos (author), Cayota, Alfonso (author)
Médium: article
Jazyk:angličtina
Vydáno: 2018
Témata:
On-line přístup:https://hdl.handle.net/20.500.12008/22005
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author Tosar Rovira, Juan Pablo
author2 Rovira, Carlos
Cayota, Alfonso
author2_role author
author
author_browse Cayota, Alfonso
Rovira, Carlos
Tosar Rovira, Juan Pablo
author_facet Tosar Rovira, Juan Pablo
Rovira, Carlos
Cayota, Alfonso
author_role author
collection COLIBRI
dc.contributor.none.fl_str_mv Tosar, Juan Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares
Cayota, Alfonso. Instituto Pasteur (Montevideo).
dc.creator.none.fl_str_mv Tosar Rovira, Juan Pablo
Rovira, Carlos
Cayota, Alfonso
dc.date.none.fl_str_mv 2018
2019-10-02T22:08:23Z
2019-10-02T22:08:23Z
20190930
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv Tosar, J.P., Rovira, C.,Cayota, A. Non-coding RNA fragments account for the majority of annotated piRNAs expressed in somatic non-gonadal tissues. Communications Biology, 2018, 1, art.nro. 2. doi: 10.1038/s42003-017-0001-7 
2399-3642
https://hdl.handle.net/20.500.12008/22005
10.1038/s42003-017-0001-7 
dc.language.none.fl_str_mv en
eng
dc.publisher.none.fl_str_mv Nature Publishing Group
dc.relation.none.fl_str_mv Communications Biology, 2018,1 , art. no. 2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Licencia Creative Commons Atribución (CC –BY 4.0)
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
dc.subject.none.fl_str_mv piRNAs
Sequence annotation
dc.title.none.fl_str_mv Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
dc.type.none.fl_str_mv Artículo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description PIWI-interacting RNAs (piRNAs) are regarded as the guardians of the genome because theytackle genome stability-threatening transposable elements in the germline. Recently, piRNAswere also reported in other types of cells, including mouse brain, malignant and non-malignant somatic tissues, and human plasma. This suggests that piRNA function might bebroader than previously expected. Here, we show that different piRNA databases contain asubset of sequences that correspond to piRNA-sized fragments of ncRNAs (rRNAs, tRNAs,YRNAs, snRNAs, and snoRNAs) and intermediates of miRNA biogenesis. We discuss that thebiogenesis of these sequences is probably independent of the PIWI pathway, and cantherefore be considered contaminants in piRNA databases. Although a minority of annotatedpiRNAs falls in this category, they account for the vast majority of piRNA expression insomatic non-gonadal tissues. Since ncRNA fragments are ubiquitous and abundant, theirconfusion with piRNAs strongly impacts the estimation of piRNA expression outside ofmammalian gonads.
eu_rights_str_mv openAccess
format article
id anni_d4e8c3b921be8bf914674b8958bcd2db
identifier_str_mv Tosar, J.P., Rovira, C.,Cayota, A. Non-coding RNA fragments account for the majority of annotated piRNAs expressed in somatic non-gonadal tissues. Communications Biology, 2018, 1, art.nro. 2. doi: 10.1038/s42003-017-0001-7 
2399-3642
10.1038/s42003-017-0001-7 
instacron_str Universidad de la República
institution Universidad de la República
instname_str Universidad de la República
language eng
language_invalid_str_mv en
network_acronym_str anni
network_name_str oai-lr-anni
oai_identifier_str oai:colibri.udelar.edu.uy:20.500.12008/22005
publishDate 2018
publishDateSort 2018
publisher.none.fl_str_mv Nature Publishing Group
reponame_str COLIBRI
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv Licencia Creative Commons Atribución (CC –BY 4.0)
spelling Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissuesTosar Rovira, Juan PabloRovira, CarlosCayota, AlfonsopiRNAsSequence annotationPIWI-interacting RNAs (piRNAs) are regarded as the guardians of the genome because theytackle genome stability-threatening transposable elements in the germline. Recently, piRNAswere also reported in other types of cells, including mouse brain, malignant and non-malignant somatic tissues, and human plasma. This suggests that piRNA function might bebroader than previously expected. Here, we show that different piRNA databases contain asubset of sequences that correspond to piRNA-sized fragments of ncRNAs (rRNAs, tRNAs,YRNAs, snRNAs, and snoRNAs) and intermediates of miRNA biogenesis. We discuss that thebiogenesis of these sequences is probably independent of the PIWI pathway, and cantherefore be considered contaminants in piRNA databases. Although a minority of annotatedpiRNAs falls in this category, they account for the vast majority of piRNA expression insomatic non-gonadal tissues. Since ncRNA fragments are ubiquitous and abundant, theirconfusion with piRNAs strongly impacts the estimation of piRNA expression outside ofmammalian gonads.Nature Publishing GroupTosar, Juan Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones NuclearesCayota, Alfonso. Instituto Pasteur (Montevideo).2019-10-02T22:08:23Z2019-10-02T22:08:23Z201820190930Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfTosar, J.P., Rovira, C.,Cayota, A. Non-coding RNA fragments account for the majority of annotated piRNAs expressed in somatic non-gonadal tissues. Communications Biology, 2018, 1, art.nro. 2. doi: 10.1038/s42003-017-0001-7 2399-3642https://hdl.handle.net/20.500.12008/2200510.1038/s42003-017-0001-7 reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengCommunications Biology, 2018,1 , art. no. 2Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad De La República. (Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC –BY 4.0)oai:colibri.udelar.edu.uy:20.500.12008/220052026-04-14T10:09:14Z
spellingShingle Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
Tosar Rovira, Juan Pablo
piRNAs
Sequence annotation
status_str publishedVersion
title Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
title_full Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
title_fullStr Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
title_full_unstemmed Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
title_short Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
title_sort Non-coding RNA fragments account for themajority of annotated piRNAs expressed in somaticnon-gonadal tissues
topic piRNAs
Sequence annotation
url https://hdl.handle.net/20.500.12008/22005