Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer
There is increasing interest among cancer researchers in the study of Piwi-interacting RNAs (piRNAs), a group of small RNAs important for maintaining genome stability in the germline. Aberrant expression of piRNAs in cancer could imply an involvement of these regulatory RNAs in neoplastic transforma...
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| Other Authors: | , |
| Format: | article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/20.500.12008/37390 |
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| _version_ | 1868890198440411136 |
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| author | Tosar Rovira, Juan Pablo |
| author2 | García Silva, María Rosa Cayota, Alfonso |
| author2_role | author author |
| author_browse | Cayota, Alfonso García Silva, María Rosa Tosar Rovira, Juan Pablo |
| author_facet | Tosar Rovira, Juan Pablo García Silva, María Rosa Cayota, Alfonso |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Tosar Rovira Juan Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. García Silva María Rosa, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Cayota Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. |
| dc.creator.none.fl_str_mv | Tosar Rovira, Juan Pablo García Silva, María Rosa Cayota, Alfonso |
| dc.date.none.fl_str_mv | 2021 2023-06-02T15:17:45Z 2023-06-02T15:17:45Z |
| dc.format.none.fl_str_mv | 9 h application/pdf |
| dc.identifier.none.fl_str_mv | Tosar Rovira, J, García Silva, M y Cayota, A. "Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer". RNA. [en línea] 2021, 27(4): 403-410. 9 h. DOI: 10.1261/rna.078444.120. 1355-8382 https://hdl.handle.net/20.500.12008/37390 10.1261/rna.078444.120 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | RNA Society |
| dc.relation.none.fl_str_mv | RNA, 2021, 27(4): 403-410. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | PIWI proteins Piwi-interacting RNAs Cancer piRNAs Somatic piRNAs piRNA databases |
| dc.title.none.fl_str_mv | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | There is increasing interest among cancer researchers in the study of Piwi-interacting RNAs (piRNAs), a group of small RNAs important for maintaining genome stability in the germline. Aberrant expression of piRNAs in cancer could imply an involvement of these regulatory RNAs in neoplastic transformation. On top of that, it could enable early cancer diagnosis based on RNA analysis in liquid biopsies, as piRNAs are not expected to widely circulate in the bloodstream of healthy individuals. Indeed, it has recently been shown that serum piR-54265 allows for excellent discrimination between colorectal cancer patients and healthy controls. However, we have also shown that most somatic piRNAs reported to date in mammals are actually fragments of other noncoding RNAs. Herein, we show that reports positioning piR-54265 as a noninvasive biomarker for colorectal cancer were actually measuring variations in the levels of a full-length (72 nt) small nucleolar RNA in serum. This should place a cautionary note for future research in somatic and cancer-specific piRNAs. We deeply encourage this line of research but discuss proper ways to identify somatic piRNAs without the interference of erroneous entries contained in piRNA databases.Wealso introduce the concept of miscellaneous-piRNAs (m-piRNAs) to distinguish between canonical piRNAs and other small RNAs circumstantially associated with PIWI proteins in somatic cells. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_d0c1a5ee927dcc3eb0c0462fa560a2b6 |
| identifier_str_mv | Tosar Rovira, J, García Silva, M y Cayota, A. "Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer". RNA. [en línea] 2021, 27(4): 403-410. 9 h. DOI: 10.1261/rna.078444.120. 1355-8382 10.1261/rna.078444.120 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/37390 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher.none.fl_str_mv | RNA Society |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| spelling | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancerTosar Rovira, Juan PabloGarcía Silva, María RosaCayota, AlfonsoPIWI proteinsPiwi-interacting RNAsCancer piRNAsSomatic piRNAspiRNA databasesThere is increasing interest among cancer researchers in the study of Piwi-interacting RNAs (piRNAs), a group of small RNAs important for maintaining genome stability in the germline. Aberrant expression of piRNAs in cancer could imply an involvement of these regulatory RNAs in neoplastic transformation. On top of that, it could enable early cancer diagnosis based on RNA analysis in liquid biopsies, as piRNAs are not expected to widely circulate in the bloodstream of healthy individuals. Indeed, it has recently been shown that serum piR-54265 allows for excellent discrimination between colorectal cancer patients and healthy controls. However, we have also shown that most somatic piRNAs reported to date in mammals are actually fragments of other noncoding RNAs. Herein, we show that reports positioning piR-54265 as a noninvasive biomarker for colorectal cancer were actually measuring variations in the levels of a full-length (72 nt) small nucleolar RNA in serum. This should place a cautionary note for future research in somatic and cancer-specific piRNAs. We deeply encourage this line of research but discuss proper ways to identify somatic piRNAs without the interference of erroneous entries contained in piRNA databases.Wealso introduce the concept of miscellaneous-piRNAs (m-piRNAs) to distinguish between canonical piRNAs and other small RNAs circumstantially associated with PIWI proteins in somatic cells.ANII: FCE_3_2018_1_ 148745ANII: FSS_X_2018_1_149070RNA SocietyTosar Rovira Juan Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.García Silva María Rosa, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Cayota Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.2023-06-02T15:17:45Z2023-06-02T15:17:45Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 happlication/pdfTosar Rovira, J, García Silva, M y Cayota, A. "Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer". RNA. [en línea] 2021, 27(4): 403-410. 9 h. DOI: 10.1261/rna.078444.120.1355-8382https://hdl.handle.net/20.500.12008/3739010.1261/rna.078444.120reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengRNA, 2021, 27(4): 403-410.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)oai:colibri.udelar.edu.uy:20.500.12008/373902026-04-14T10:10:05Z |
| spellingShingle | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer Tosar Rovira, Juan Pablo PIWI proteins Piwi-interacting RNAs Cancer piRNAs Somatic piRNAs piRNA databases |
| status_str | publishedVersion |
| title | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| title_full | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| title_fullStr | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| title_full_unstemmed | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| title_short | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| title_sort | Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer |
| topic | PIWI proteins Piwi-interacting RNAs Cancer piRNAs Somatic piRNAs piRNA databases |
| url | https://hdl.handle.net/20.500.12008/37390 |