Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates
Antibiotic resistance is an alarming problem throughout the world and carbapenemresistant Pseudomonas aeruginosa has been cataloged as critical in the World Health Organization list of microorganisms in urgent need for the development of new antimicrobials. In this work, we describe two novel resist...
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| Format: | article |
| Idioma: | anglès |
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2023
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| Accés en línia: | https://hdl.handle.net/20.500.12008/50768 |
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| _version_ | 1868890194452676608 |
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| author | Papa-Ezdra, Romina |
| author2 | Cordeiro, Nicolás F. Outeda, Matilde Garcia-Fulgueiras, Virginia Araújo, Lucía Seija, Verónica Ayala, Juan A. Bado, Inés Vignoli, Rafael |
| author2_role | author author author author author author author author |
| author_browse | Araújo, Lucía Ayala, Juan A. Bado, Inés Cordeiro, Nicolás F. Garcia-Fulgueiras, Virginia Outeda, Matilde Papa-Ezdra, Romina Seija, Verónica Vignoli, Rafael |
| author_facet | Papa-Ezdra, Romina Cordeiro, Nicolás F. Outeda, Matilde Garcia-Fulgueiras, Virginia Araújo, Lucía Seija, Verónica Ayala, Juan A. Bado, Inés Vignoli, Rafael |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Papa-Ezdra Romina, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Cordeiro Nicolás F., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Outeda Matilde, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Unidad Académica Laboratorio Clínico. Área Microbiología. Garcia-Fulgueiras Virginia, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Araújo Lucía, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Seija Verónica, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Unidad Académica Laboratorio Clínico. Área Microbiología. Ayala Juan A., Centro de Biología Molecular “Severo Ochoa” (CBMSO)-CSIC (España) Bado Inés, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Vignoli Rafael, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología |
| dc.creator.none.fl_str_mv | Papa-Ezdra, Romina Cordeiro, Nicolás F. Outeda, Matilde Garcia-Fulgueiras, Virginia Araújo, Lucía Seija, Verónica Ayala, Juan A. Bado, Inés Vignoli, Rafael |
| dc.date.none.fl_str_mv | 2023 2025-07-25T15:05:58Z 2025-07-25T15:05:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | PAPA-EZDRA, R., CORDEIRO, NF., OUTEDA, M., y otros. Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates. Antibiotics [en línea] 2023, 12. DOI: 10.3390/antibiotics12020304 https://hdl.handle.net/20.500.12008/50768 10.3390/antibiotics12020304 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Antibiotics. 12, 2023 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Pseudomonas aeruginosa blaVIM-2 blaPER-1 Transposon |
| dc.title.none.fl_str_mv | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Antibiotic resistance is an alarming problem throughout the world and carbapenemresistant Pseudomonas aeruginosa has been cataloged as critical in the World Health Organization list of microorganisms in urgent need for the development of new antimicrobials. In this work, we describe two novel resistance regions responsible for conferring a multidrug resistance phenotype to two clinical isolates of P. aeruginosa (Pa873 and Pa6415) obtained from patients hospitalized in the ICU of University Hospital of Uruguay. Bacterial identification and antibiotic susceptibility tests were performed using MALDI-TOF and the Vitek 2 system, respectively. WGS was performed for both isolates using Oxford Nanopore Technologies and Illumina and processed by means of hybrid assembly. Both isolates were resistant to ceftazidime, cefepime, piperacillin–tazobactam, aztreonam, and imipenem. Strain Pa6415 also showed resistance to ciprofloxacin. Both strains displayed MICs below the susceptibility breakpoint for CAZ-AVI plus 4 mg/L of aztreonam as well as cefiderocol. Both resistance regions are flanked by the left and right inverted repeats of ISPa40 in two small regions spanning 39.3 and 35.6 kb, for Pa6415 and Pa873, respectively. The resistance region of Pa6415 includes TnaphA6, and the new Tn7516 consists of IRi, In899, qacE∆1-sul1-ISCR1, qnrVC6-ISCR1- blaPER-1-qacE∆1-sul1, araJ-like, IS481-like tnpA, ISPa17, and IRR. On the other hand, the resistance region of Pa873 includes Tnaph6 and the new Tn7517 (IRi, In899, qacE∆1-sul1, ISCR1–blaPER-1–qacE∆1- sul1, araJ-like, IS481-like tnpA, ISPa17, and IRR). It is necessary to monitor the emergence of genetic structures that threaten to invalidate the available therapeutic resources. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_cd14c63b24eb5be93e0a79e5ca6b1d2d |
| identifier_str_mv | PAPA-EZDRA, R., CORDEIRO, NF., OUTEDA, M., y otros. Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates. Antibiotics [en línea] 2023, 12. DOI: 10.3390/antibiotics12020304 10.3390/antibiotics12020304 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50768 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical IsolatesPapa-Ezdra, RominaCordeiro, Nicolás F.Outeda, MatildeGarcia-Fulgueiras, VirginiaAraújo, LucíaSeija, VerónicaAyala, Juan A.Bado, InésVignoli, RafaelPseudomonas aeruginosablaVIM-2blaPER-1TransposonAntibiotic resistance is an alarming problem throughout the world and carbapenemresistant Pseudomonas aeruginosa has been cataloged as critical in the World Health Organization list of microorganisms in urgent need for the development of new antimicrobials. In this work, we describe two novel resistance regions responsible for conferring a multidrug resistance phenotype to two clinical isolates of P. aeruginosa (Pa873 and Pa6415) obtained from patients hospitalized in the ICU of University Hospital of Uruguay. Bacterial identification and antibiotic susceptibility tests were performed using MALDI-TOF and the Vitek 2 system, respectively. WGS was performed for both isolates using Oxford Nanopore Technologies and Illumina and processed by means of hybrid assembly. Both isolates were resistant to ceftazidime, cefepime, piperacillin–tazobactam, aztreonam, and imipenem. Strain Pa6415 also showed resistance to ciprofloxacin. Both strains displayed MICs below the susceptibility breakpoint for CAZ-AVI plus 4 mg/L of aztreonam as well as cefiderocol. Both resistance regions are flanked by the left and right inverted repeats of ISPa40 in two small regions spanning 39.3 and 35.6 kb, for Pa6415 and Pa873, respectively. The resistance region of Pa6415 includes TnaphA6, and the new Tn7516 consists of IRi, In899, qacE∆1-sul1-ISCR1, qnrVC6-ISCR1- blaPER-1-qacE∆1-sul1, araJ-like, IS481-like tnpA, ISPa17, and IRR. On the other hand, the resistance region of Pa873 includes Tnaph6 and the new Tn7517 (IRi, In899, qacE∆1-sul1, ISCR1–blaPER-1–qacE∆1- sul1, araJ-like, IS481-like tnpA, ISPa17, and IRR). It is necessary to monitor the emergence of genetic structures that threaten to invalidate the available therapeutic resources.Papa-Ezdra Romina, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaCordeiro Nicolás F., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaOuteda Matilde, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Unidad Académica Laboratorio Clínico. Área Microbiología.Garcia-Fulgueiras Virginia, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaAraújo Lucía, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaSeija Verónica, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Unidad Académica Laboratorio Clínico. Área Microbiología.Ayala Juan A., Centro de Biología Molecular “Severo Ochoa” (CBMSO)-CSIC (España)Bado Inés, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaVignoli Rafael, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología2025-07-25T15:05:58Z2025-07-25T15:05:58Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfPAPA-EZDRA, R., CORDEIRO, NF., OUTEDA, M., y otros. Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates. Antibiotics [en línea] 2023, 12. DOI: 10.3390/antibiotics12020304https://hdl.handle.net/20.500.12008/5076810.3390/antibiotics12020304reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengAntibiotics. 12, 2023Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/507682026-04-14T10:37:02Z |
| spellingShingle | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates Papa-Ezdra, Romina Pseudomonas aeruginosa blaVIM-2 blaPER-1 Transposon |
| status_str | publishedVersion |
| title | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| title_full | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| title_fullStr | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| title_full_unstemmed | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| title_short | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| title_sort | Novel Resistance Regions Carrying TnaphA6, blaVIM-2, and blaPER-1, Embedded in an ISPa40-Derived Transposon from Two Multi-Resistant Pseudomonas aeruginosa Clinical Isolates |
| topic | Pseudomonas aeruginosa blaVIM-2 blaPER-1 Transposon |
| url | https://hdl.handle.net/20.500.12008/50768 |