Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs tr...
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| Autres auteurs: | , , , , , |
| Format: | article |
| Langue: | anglais |
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2020
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| Accès en ligne: | https://hdl.handle.net/20.500.12008/31776 |
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| _version_ | 1868890189423706112 |
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| author | Canclini, Lucía |
| author2 | Farías, Joaquina Di Paolo, Andrés Sotelo-Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| author2_role | author author author author author author |
| author_browse | Canclini, Lucía Di Paolo, Andrés Farías, Joaquina Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto Sotelo-Silveira, José Roberto |
| author_facet | Canclini, Lucía Farías, Joaquina Di Paolo, Andrés Sotelo-Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Canclini Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Farías Joaquina, IIBCE Di Paolo Forné Andrés Pablo, IIBCE Sotelo-Silveira José Roberto, IIBCE Folle Ungo Gustavo A., IIBCE Kun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Sotelo Sosa José Roberto, IIBCE |
| dc.creator.none.fl_str_mv | Canclini, Lucía Farías, Joaquina Di Paolo, Andrés Sotelo-Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| dc.date.none.fl_str_mv | 2020 2022-06-01T13:37:37Z 2022-06-01T13:37:37Z |
| dc.format.none.fl_str_mv | 18 h. application/pdf |
| dc.identifier.none.fl_str_mv | Canclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.0233651 1932-6203 https://hdl.handle.net/20.500.12008/31776 10.1371/journal.pone.0233651 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Public Library of Science |
| dc.relation.none.fl_str_mv | PLoS ONE, 2020, 15(5): e0233651 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| dc.title.none.fl_str_mv | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_c7397795865e7ed99bc7eebc1833c6f0 |
| identifier_str_mv | Canclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.0233651 1932-6203 10.1371/journal.pone.0233651 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31776 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | Public Library of Science |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerveCanclini, LucíaFarías, JoaquinaDi Paolo, AndrésSotelo-Silveira, José RobertoFolle Ungo, Gustavo A.Kun González, Alejandra E.Sotelo Sosa, José RobertoRNAAxonsMicrotubulesNerve RegenerationSchwann CellsSciatic NerveTransference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors.Public Library of ScienceCanclini Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFarías Joaquina, IIBCEDi Paolo Forné Andrés Pablo, IIBCESotelo-Silveira José Roberto, IIBCEFolle Ungo Gustavo A., IIBCEKun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaSotelo Sosa José Roberto, IIBCE2022-06-01T13:37:37Z2022-06-01T13:37:37Z2020Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 h.application/pdfCanclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.02336511932-6203https://hdl.handle.net/20.500.12008/3177610.1371/journal.pone.0233651reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengPLoS ONE, 2020, 15(5): e0233651Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/317762026-04-14T10:09:48Z |
| spellingShingle | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve Canclini, Lucía RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| status_str | publishedVersion |
| title | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_full | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_fullStr | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_full_unstemmed | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_short | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_sort | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| topic | RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| url | https://hdl.handle.net/20.500.12008/31776 |