Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
Background: Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are cu...
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| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/20.500.12008/50559 |
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| _version_ | 1868890184604450816 |
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| author | Giner-Lamia, Joaquín |
| author2 | Vinuesa, Pablo Betancor, Laura Silva, Claudia Bisio, Julieta Soleto, Lorena Chabalgoity, José A. Puente, José Luis García-del Portillo, Francisco |
| author2_role | author author author author author author author author |
| author_browse | Betancor, Laura Bisio, Julieta Chabalgoity, José A. García-del Portillo, Francisco Giner-Lamia, Joaquín Puente, José Luis Silva, Claudia Soleto, Lorena Vinuesa, Pablo |
| author_facet | Giner-Lamia, Joaquín Vinuesa, Pablo Betancor, Laura Silva, Claudia Bisio, Julieta Soleto, Lorena Chabalgoity, José A. Puente, José Luis García-del Portillo, Francisco |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Giner-Lamia Joaquín, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC) (España). Laboratorio de Patógenos Bacterianos Intracelulares Vinuesa Pablo, Universidad Nacional Autónoma de México (México). Centro de Ciencias Genómicas Betancor Laura, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología Silva Claudia, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología Molecular Bisio Julieta, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología Molecular Soleto Lorena, Ministerio de Salud de Bolivia (Bolivia). Centro Nacional de Enfermedades Tropicales (CENETROP) Chabalgoity José A., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Desarrollo Biotecnológico Puente José Luis, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología Molecular García-del Portillo Francisco, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC) (España). Laboratorio de Patógenos Bacterianos Intracelulares |
| dc.creator.none.fl_str_mv | Giner-Lamia, Joaquín Vinuesa, Pablo Betancor, Laura Silva, Claudia Bisio, Julieta Soleto, Lorena Chabalgoity, José A. Puente, José Luis García-del Portillo, Francisco |
| dc.date.none.fl_str_mv | 2019 2025-07-11T16:16:49Z 2025-07-11T16:16:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | GINER-LAMIA, J., VINUESA, P., BETANCOR, L., y otros. "Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256". BMC Genomics [en línea] 2019, 20. DOI: 10.1186/s12864-018-5352-z https://hdl.handle.net/20.500.12008/50559 10.1186/s12864-018-5352-z |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | BMC Genomics. 20, 2019 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Salmonella enterica Subspecies diarizonae Invasive human infections Comparative genomics Virulence genes Type-III effectors |
| dc.title.none.fl_str_mv | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Background: Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results: We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport relatedgenes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions: The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_c1c4f7f35b964dfe4eb425335fbbfed3 |
| identifier_str_mv | GINER-LAMIA, J., VINUESA, P., BETANCOR, L., y otros. "Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256". BMC Genomics [en línea] 2019, 20. DOI: 10.1186/s12864-018-5352-z 10.1186/s12864-018-5352-z |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50559 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256Giner-Lamia, JoaquínVinuesa, PabloBetancor, LauraSilva, ClaudiaBisio, JulietaSoleto, LorenaChabalgoity, José A.Puente, José LuisGarcía-del Portillo, FranciscoSalmonella entericaSubspecies diarizonaeInvasive human infectionsComparative genomicsVirulence genesType-III effectorsBackground: Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results: We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport relatedgenes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions: The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host.Comisión Sectorial de Investigación Científica (CSIC)Giner-Lamia Joaquín, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC) (España). Laboratorio de Patógenos Bacterianos IntracelularesVinuesa Pablo, Universidad Nacional Autónoma de México (México). Centro de Ciencias GenómicasBetancor Laura, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y VirologíaSilva Claudia, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología MolecularBisio Julieta, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología MolecularSoleto Lorena, Ministerio de Salud de Bolivia (Bolivia). Centro Nacional de Enfermedades Tropicales (CENETROP)Chabalgoity José A., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Desarrollo BiotecnológicoPuente José Luis, Universidad Nacional Autónoma de México (México). Instituto de Biotecnología. Departamento de Microbiología MolecularGarcía-del Portillo Francisco, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC) (España). Laboratorio de Patógenos Bacterianos Intracelulares2025-07-11T16:16:49Z2025-07-11T16:16:49Z2019Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfGINER-LAMIA, J., VINUESA, P., BETANCOR, L., y otros. "Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256". BMC Genomics [en línea] 2019, 20. DOI: 10.1186/s12864-018-5352-zhttps://hdl.handle.net/20.500.12008/5055910.1186/s12864-018-5352-zreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengBMC Genomics. 20, 2019Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/505592026-04-14T10:37:01Z |
| spellingShingle | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 Giner-Lamia, Joaquín Salmonella enterica Subspecies diarizonae Invasive human infections Comparative genomics Virulence genes Type-III effectors |
| status_str | publishedVersion |
| title | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| title_full | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| title_fullStr | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| title_full_unstemmed | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| title_short | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| title_sort | Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256 |
| topic | Salmonella enterica Subspecies diarizonae Invasive human infections Comparative genomics Virulence genes Type-III effectors |
| url | https://hdl.handle.net/20.500.12008/50559 |