Intra-colonial viral infections in western honey bees (Apis Mellifera)
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two mai...
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| Format: | article |
| Sprog: | engelsk |
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2021
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| Online adgang: | https://hdl.handle.net/20.500.12008/41278 |
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| _version_ | 1868890178832039936 |
|---|---|
| author | Castelli Norando, Loreley |
| author2 | Genchi-García, María Laura Dalmon, Anne Arredondo Papiol, Daniela Antúnez, Karina Invernizzi Castillo, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
| author2_role | author author author author author author author author |
| author_browse | Antúnez, Karina Arredondo Papiol, Daniela Beaurepaire, Alexis Castelli Norando, Loreley Dalmon, Anne Genchi-García, María Laura Invernizzi Castillo, Ciro Le Conte, Yves Reynaldi, Francisco José |
| author_facet | Castelli Norando, Loreley Genchi-García, María Laura Dalmon, Anne Arredondo Papiol, Daniela Antúnez, Karina Invernizzi Castillo, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Castelli Norando Loreley, IIBCE Genchi-García María Laura Dalmon Anne Arredondo Papiol Daniela, IIBCE Antúnez Karina, IIBCE Invernizzi Castillo Ciro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Reynaldi Francisco José Le Conte Yves Beaurepaire Alexis |
| dc.creator.none.fl_str_mv | Castelli Norando, Loreley Genchi-García, María Laura Dalmon, Anne Arredondo Papiol, Daniela Antúnez, Karina Invernizzi Castillo, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
| dc.date.none.fl_str_mv | 2021 2023-11-16T12:11:29Z 2023-11-16T12:11:29Z |
| dc.format.none.fl_str_mv | 12 h. application/pdf |
| dc.identifier.none.fl_str_mv | Castelli Norando, L, Genchi-García, M, Dalmon, A [y otros autores]. "Intra-colonial viral infections in western honey bees (Apis Mellifera)". Microorganisms. [en línea] 2021, 9(5): 1087. 12 h. DOI: 10.3390/microorganisms9051087. 2076-2607 https://hdl.handle.net/20.500.12008/41278 10.3390/microorganisms9051087 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Microorganisms, 2021, 9(5): 1087. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Evolutionary biology Host–pathogen interactions Population genetics Viruses Pollinators |
| dc.title.none.fl_str_mv | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_bd96c6e603e8ef59f1f7ceeffd06e941 |
| identifier_str_mv | Castelli Norando, L, Genchi-García, M, Dalmon, A [y otros autores]. "Intra-colonial viral infections in western honey bees (Apis Mellifera)". Microorganisms. [en línea] 2021, 9(5): 1087. 12 h. DOI: 10.3390/microorganisms9051087. 2076-2607 10.3390/microorganisms9051087 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/41278 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Intra-colonial viral infections in western honey bees (Apis Mellifera)Castelli Norando, LoreleyGenchi-García, María LauraDalmon, AnneArredondo Papiol, DanielaAntúnez, KarinaInvernizzi Castillo, CiroReynaldi, Francisco JoséLe Conte, YvesBeaurepaire, AlexisEvolutionary biologyHost–pathogen interactionsPopulation geneticsVirusesPollinatorsRNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.Castelli Norando Loreley, IIBCEGenchi-García María LauraDalmon AnneArredondo Papiol Daniela, IIBCEAntúnez Karina, IIBCEInvernizzi Castillo Ciro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Reynaldi Francisco JoséLe Conte YvesBeaurepaire Alexis2023-11-16T12:11:29Z2023-11-16T12:11:29Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 h.application/pdfCastelli Norando, L, Genchi-García, M, Dalmon, A [y otros autores]. "Intra-colonial viral infections in western honey bees (Apis Mellifera)". Microorganisms. [en línea] 2021, 9(5): 1087. 12 h. DOI: 10.3390/microorganisms9051087.2076-2607https://hdl.handle.net/20.500.12008/4127810.3390/microorganisms9051087reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengMicroorganisms, 2021, 9(5): 1087.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/412782026-04-14T10:10:20Z |
| spellingShingle | Intra-colonial viral infections in western honey bees (Apis Mellifera) Castelli Norando, Loreley Evolutionary biology Host–pathogen interactions Population genetics Viruses Pollinators |
| status_str | publishedVersion |
| title | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| title_full | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| title_fullStr | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| title_full_unstemmed | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| title_short | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| title_sort | Intra-colonial viral infections in western honey bees (Apis Mellifera) |
| topic | Evolutionary biology Host–pathogen interactions Population genetics Viruses Pollinators |
| url | https://hdl.handle.net/20.500.12008/41278 |