Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons
Axonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in steady state. Recent studies have begun to identify the mRNAs localized in axons, which could be translated locally under different conditions. Despite th...
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| Other Authors: | , , |
| Format: | article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/20.500.12008/31769 |
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| _version_ | 1868890173168680960 |
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| author | Farías, Joaquina |
| author2 | Holt, C. E. Sotelo Sosa, José Roberto Sotelo-Silveira, José Roberto |
| author2_role | author author author |
| author_browse | Farías, Joaquina Holt, C. E. Sotelo Sosa, José Roberto Sotelo-Silveira, José Roberto |
| author_facet | Farías, Joaquina Holt, C. E. Sotelo Sosa, José Roberto Sotelo-Silveira, José Roberto |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Farías Joaquina, IIBCE Holt C. E. Sotelo Sosa José Roberto, IIBCE Sotelo Silveira José Roberto, IIBCE |
| dc.creator.none.fl_str_mv | Farías, Joaquina Holt, C. E. Sotelo Sosa, José Roberto Sotelo-Silveira, José Roberto |
| dc.date.none.fl_str_mv | 2020 2022-06-01T11:41:09Z 2022-06-01T11:41:09Z |
| dc.format.none.fl_str_mv | 52 h. application/pdf |
| dc.identifier.none.fl_str_mv | Farías, J, Holt, C, Sotelo, J. [y otros] "Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons". RNA. [en línea] 2020, 26: 595-612 . 52 h. DOI: 10.1261/rna.073700.119 1469-9001 https://hdl.handle.net/20.500.12008/31769 10.1261/rna.073700.119 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | RNA Society |
| dc.relation.none.fl_str_mv | RNA, 2020, 26: 595-612 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Axonal mRNAs mRNA localization Motor neuron Local translation Axon |
| dc.title.none.fl_str_mv | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Axonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in steady state. Recent studies have begun to identify the mRNAs localized in axons, which could be translated locally under different conditions. Despite that now hundreds or thousands of mRNAs have been shown to be localized into the axonal compartment of cultured neurons in vitro, knowledge of which mRNAs are localized in mature myelinated axons is quite limited. With the purpose of characterizing the transcriptome of mature myelinated motor axons of peripheral nervous system, we modified the axon micro-dissection method devised by Koenig, enabling the isolation of the axoplasm RNA to perform RNA-seq analysis. The transcriptome analysis indicates that the number of RNAs detected in mature axons is lower in comparison with in vitro data, is depleted of glial markers and enriched in neuronal markers. The mature myelinated axons are enriched for mRNAs related to cytoskeleton, translation and oxidative phosphorylation. Moreover, it was possible to define core genes present in axons when comparing our data with transcriptomic data of axons grown in different conditions. This work provides evidence that axon micro-dissection is a valuable method to obtain data at genome-wide levels of mature and myelinated axons of the peripheral nervous system, and could be especially useful for the study of axonal involvement in neurodegenerative pathologies of motor neurons such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophies (SMA). Farias |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_b64bc68a755da3b3d5887647fa4c7c4a |
| identifier_str_mv | Farías, J, Holt, C, Sotelo, J. [y otros] "Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons". RNA. [en línea] 2020, 26: 595-612 . 52 h. DOI: 10.1261/rna.073700.119 1469-9001 10.1261/rna.073700.119 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31769 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | RNA Society |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axonsFarías, JoaquinaHolt, C. E.Sotelo Sosa, José RobertoSotelo-Silveira, José RobertoAxonal mRNAsmRNA localizationMotor neuronLocal translationAxonAxonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in steady state. Recent studies have begun to identify the mRNAs localized in axons, which could be translated locally under different conditions. Despite that now hundreds or thousands of mRNAs have been shown to be localized into the axonal compartment of cultured neurons in vitro, knowledge of which mRNAs are localized in mature myelinated axons is quite limited. With the purpose of characterizing the transcriptome of mature myelinated motor axons of peripheral nervous system, we modified the axon micro-dissection method devised by Koenig, enabling the isolation of the axoplasm RNA to perform RNA-seq analysis. The transcriptome analysis indicates that the number of RNAs detected in mature axons is lower in comparison with in vitro data, is depleted of glial markers and enriched in neuronal markers. The mature myelinated axons are enriched for mRNAs related to cytoskeleton, translation and oxidative phosphorylation. Moreover, it was possible to define core genes present in axons when comparing our data with transcriptomic data of axons grown in different conditions. This work provides evidence that axon micro-dissection is a valuable method to obtain data at genome-wide levels of mature and myelinated axons of the peripheral nervous system, and could be especially useful for the study of axonal involvement in neurodegenerative pathologies of motor neurons such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophies (SMA). FariasRNA SocietyFarías Joaquina, IIBCEHolt C. E.Sotelo Sosa José Roberto, IIBCESotelo Silveira José Roberto, IIBCE2022-06-01T11:41:09Z2022-06-01T11:41:09Z2020Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion52 h.application/pdfFarías, J, Holt, C, Sotelo, J. [y otros] "Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons". RNA. [en línea] 2020, 26: 595-612 . 52 h. DOI: 10.1261/rna.073700.1191469-9001https://hdl.handle.net/20.500.12008/3176910.1261/rna.073700.119reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengRNA, 2020, 26: 595-612Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/317692026-04-14T10:09:47Z |
| spellingShingle | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons Farías, Joaquina Axonal mRNAs mRNA localization Motor neuron Local translation Axon |
| status_str | publishedVersion |
| title | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| title_full | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| title_fullStr | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| title_full_unstemmed | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| title_short | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| title_sort | Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons |
| topic | Axonal mRNAs mRNA localization Motor neuron Local translation Axon |
| url | https://hdl.handle.net/20.500.12008/31769 |