Rapid preparation of rodent testicular cell suspensions and spermatogenic stages purification by flow cytometry using a novel blue-laser-excitable vital dye

Availability of purified or highly enriched fractions representing the various spermatogenic stages is a usual requirement to study mammalian spermatogenesis at the molecular level. Fast preparation of high quality testicular cell suspensions is crucial when flow cytometry (FCM) is chosen to accompl...

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Bibliographic Details
Main Author: Rodríguez-Casuriaga, Rosana (author)
Other Authors: Santiñaque, Federico F. (author), Folle, Gustavo A. (author), Souza, Elisa (author), López-Carro, Beatriz (author), Geisinger, Adriana (author)
Format: article
Language:English
Published: 2014
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Online Access:http://hdl.handle.net/20.500.12381/130
http://dx.doi.org/10.1016/j.mex.2014.10.002
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