Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated la...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/48404 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890160348790784 |
|---|---|
| author | Viotti Cresci, María Helen |
| author2 | Juri, Pablo Presentado, María Laura Verdes García, José Manuel Nogueira, Enrique Invernizzi, Ciro Pedrana, Graciela |
| author2_role | author author author author author author |
| author_browse | Invernizzi, Ciro Juri, Pablo Nogueira, Enrique Pedrana, Graciela Presentado, María Laura Verdes García, José Manuel Viotti Cresci, María Helen |
| author_facet | Viotti Cresci, María Helen Juri, Pablo Presentado, María Laura Verdes García, José Manuel Nogueira, Enrique Invernizzi, Ciro Pedrana, Graciela |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Viotti Cresci María Helen, Universidad de la República (Uruguay). Facultad de Veterinaria Juri Pablo, Universidad de la República (Uruguay). Facultad de Veterinaria Presentado María Laura, Universidad de la República (Uruguay). Facultad de Veterinaria Verdes García José Manuel, Universidad de la República (Uruguay). Facultad de Veterinaria Nogueira Enrique, Universidad de la República (Uruguay). Facultad de Veterinaria Invernizzi Ciro, Universidad de la República (Uruguay). Facultad de Veterinaria Pedrana Graciela, Universidad de la República (Uruguay). Facultad de Veterinaria |
| dc.creator.none.fl_str_mv | Viotti Cresci, María Helen Juri, Pablo Presentado, María Laura Verdes García, José Manuel Nogueira, Enrique Invernizzi, Ciro Pedrana, Graciela |
| dc.date.none.fl_str_mv | 2021 2025-02-14T17:35:10Z 2025-02-14T17:35:10Z |
| dc.format.none.fl_str_mv | 8 p application/pdf |
| dc.identifier.none.fl_str_mv | Viotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108 https://hdl.handle.net/20.500.12008/48404 10.2478/jas-2021-0006 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Journal of Apicultural Science, 2021, 65(1), 101-108 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | ABEJAS APIS MELLIFERA APICULTURA ENFERMEDADES INTOXICACION |
| dc.title.none.fl_str_mv | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p<0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p<0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p<0.002) and trophocytes (p<0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in A. mellifera larvae. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_aa92b48a0636f068fb3df2e0e7eb204c |
| identifier_str_mv | Viotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108 10.2478/jas-2021-0006 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/48404 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestriViotti Cresci, María HelenJuri, PabloPresentado, María LauraVerdes García, José ManuelNogueira, EnriqueInvernizzi, CiroPedrana, GracielaABEJASAPIS MELLIFERAAPICULTURAENFERMEDADESINTOXICACIONToxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p<0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p<0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p<0.002) and trophocytes (p<0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in A. mellifera larvae.Viotti Cresci María Helen, Universidad de la República (Uruguay). Facultad de VeterinariaJuri Pablo, Universidad de la República (Uruguay). Facultad de VeterinariaPresentado María Laura, Universidad de la República (Uruguay). Facultad de VeterinariaVerdes García José Manuel, Universidad de la República (Uruguay). Facultad de VeterinariaNogueira Enrique, Universidad de la República (Uruguay). Facultad de VeterinariaInvernizzi Ciro, Universidad de la República (Uruguay). Facultad de VeterinariaPedrana Graciela, Universidad de la República (Uruguay). Facultad de Veterinaria2025-02-14T17:35:10Z2025-02-14T17:35:10Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 papplication/pdfViotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108https://hdl.handle.net/20.500.12008/4840410.2478/jas-2021-0006reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Apicultural Science, 2021, 65(1), 101-108Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/484042026-04-14T10:35:06Z |
| spellingShingle | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri Viotti Cresci, María Helen ABEJAS APIS MELLIFERA APICULTURA ENFERMEDADES INTOXICACION |
| status_str | publishedVersion |
| title | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| title_full | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| title_fullStr | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| title_full_unstemmed | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| title_short | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| title_sort | Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri |
| topic | ABEJAS APIS MELLIFERA APICULTURA ENFERMEDADES INTOXICACION |
| url | https://hdl.handle.net/20.500.12008/48404 |