Detection and genotyping of equid herpesvirus 1 in Uruguay
Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | article |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/34150 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890159959769088 |
|---|---|
| author | Arbiza, Juan |
| author2 | Castro, E. R. |
| author2_role | author |
| author_browse | Arbiza, Juan Castro, E. R. |
| author_facet | Arbiza, Juan Castro, E. R. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Castro E. R. |
| dc.creator.none.fl_str_mv | Arbiza, Juan Castro, E. R. |
| dc.date.none.fl_str_mv | 2017 2022-10-13T12:29:36Z 2022-10-13T12:29:36Z |
| dc.format.none.fl_str_mv | 8 h. application/pdf |
| dc.identifier.none.fl_str_mv | Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h. 0253-1933 https://hdl.handle.net/20.500.12008/34150 10.20506/rst36.3.2715 |
| dc.language.none.fl_str_mv | en_US eng |
| dc.publisher.none.fl_str_mv | Office International des Epizootes |
| dc.relation.none.fl_str_mv | OIE Revue Scientifique et Technique, 2017, 36 (3): 799-806. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Equid herpesvirus Genotyping Lymph node Molecular detection Neuropathogenic Uruguay |
| dc.title.none.fl_str_mv | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In recent years, an increase in the number of cases of equine neurological disease caused by neuropathogenic variants of EHV-1 has been observed in numerous countries. The purpose of this study was to detect the presence of the viral genome of EHV-1 and equid herpesvirus 4 (EHV-4) in the bronchopulmonary lymph nodes of 47 horses from various locations in Uruguay, obtained from a slaughterhouse, and to determine whether the EHV-1 genomes possessed the mutation associated with neuropathogenesis (G2254/D752). The genes encoding glycoprotein H (gH) of EHV-1 and glycoprotein B (gB) of EHV-4 were amplified by a semi-nested polymerase chain reaction. Of the samples analysed, 28% and 6% of lymph nodes contained the genes for gH and gB, respectively. The viral DNA olymerase gene was amplified and sequenced. Twelve of the 13 genomes sequenced presented the nucleotide G2254, while the remaining 1 showed both nucleotides, A2254 and G2254. The results confirm the presence of EHV-1 in Uruguay. Furthermore, there is evidence for the first time of the detection of EHV- 4, and high-frequency detection of the neuropathogenic variant (G2254/D752) of EHV-1 in Uruguay. These findings provide new insights into the epidemiological situation of EHV-1 and EHV-4 in that country. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_aa5b493d162bf64cbfe200d685d3daa3 |
| identifier_str_mv | Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h. 0253-1933 10.20506/rst36.3.2715 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en_US |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/34150 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher.none.fl_str_mv | Office International des Epizootes |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Detection and genotyping of equid herpesvirus 1 in UruguayArbiza, JuanCastro, E. R.Equid herpesvirusGenotypingLymph nodeMolecular detectionNeuropathogenicUruguayInfection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In recent years, an increase in the number of cases of equine neurological disease caused by neuropathogenic variants of EHV-1 has been observed in numerous countries. The purpose of this study was to detect the presence of the viral genome of EHV-1 and equid herpesvirus 4 (EHV-4) in the bronchopulmonary lymph nodes of 47 horses from various locations in Uruguay, obtained from a slaughterhouse, and to determine whether the EHV-1 genomes possessed the mutation associated with neuropathogenesis (G2254/D752). The genes encoding glycoprotein H (gH) of EHV-1 and glycoprotein B (gB) of EHV-4 were amplified by a semi-nested polymerase chain reaction. Of the samples analysed, 28% and 6% of lymph nodes contained the genes for gH and gB, respectively. The viral DNA olymerase gene was amplified and sequenced. Twelve of the 13 genomes sequenced presented the nucleotide G2254, while the remaining 1 showed both nucleotides, A2254 and G2254. The results confirm the presence of EHV-1 in Uruguay. Furthermore, there is evidence for the first time of the detection of EHV- 4, and high-frequency detection of the neuropathogenic variant (G2254/D752) of EHV-1 in Uruguay. These findings provide new insights into the epidemiological situation of EHV-1 and EHV-4 in that country.Office International des EpizootesArbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Castro E. R.2022-10-13T12:29:36Z2022-10-13T12:29:36Z2017Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 h.application/pdfArbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h.0253-1933https://hdl.handle.net/20.500.12008/3415010.20506/rst36.3.2715reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la Repúblicaen_USengOIE Revue Scientifique et Technique, 2017, 36 (3): 799-806.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/341502026-04-14T10:09:59Z |
| spellingShingle | Detection and genotyping of equid herpesvirus 1 in Uruguay Arbiza, Juan Equid herpesvirus Genotyping Lymph node Molecular detection Neuropathogenic Uruguay |
| status_str | publishedVersion |
| title | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| title_full | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| title_fullStr | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| title_full_unstemmed | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| title_short | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| title_sort | Detection and genotyping of equid herpesvirus 1 in Uruguay |
| topic | Equid herpesvirus Genotyping Lymph node Molecular detection Neuropathogenic Uruguay |
| url | https://hdl.handle.net/20.500.12008/34150 |