Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics
The metabolic alterations associated with the increase in milk production make the transition period critical to the health of dairy cows, usually leading to a higher incidence of disease in periparturient animals. In this manuscript, we describe the use of NMR-based untargeted metabolomics to follo...
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| Médium: | article |
| Jazyk: | angličtina |
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2021
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| On-line přístup: | https://hdl.handle.net/20.500.12008/50598 https://doi.org/10.3390/dairy2030028 |
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| _version_ | 1868890158307213312 |
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| author | López Radcenco, Andrés |
| author2 | Adrien Delgado, María de Lourdes Ruprechter Schölderle, Gretel Cristina Torres, Elena de Meikle, Ana Moyna, Guillermo |
| author2_role | author author author author author |
| author_browse | Adrien Delgado, María de Lourdes López Radcenco, Andrés Meikle, Ana Moyna, Guillermo Ruprechter Schölderle, Gretel Cristina Torres, Elena de |
| author_facet | López Radcenco, Andrés Adrien Delgado, María de Lourdes Ruprechter Schölderle, Gretel Cristina Torres, Elena de Meikle, Ana Moyna, Guillermo |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | López Radcenco Andrés, Universidad de la República (Uruguay). CENUR Litoral Norte. Departamento de Química del Litoral Adrien Delgado María de Lourdes, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Salud en los Sistemas Pecuarios Ruprechter Schölderle Gretel Cristina, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Endocrinología y Metabolismo Animal Torres Elena de, Universidad de la República (Uruguay). Facultad de Veterinaria. Campo Experimental 2 Meikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Endocrinología y Metabolismo Animal Moyna Guillermo, Universidad de la República (Uruguay). CENUR Litoral Norte. Departamento de Química del Litoral |
| dc.creator.none.fl_str_mv | López Radcenco, Andrés Adrien Delgado, María de Lourdes Ruprechter Schölderle, Gretel Cristina Torres, Elena de Meikle, Ana Moyna, Guillermo |
| dc.date.none.fl_str_mv | 2021 2025-07-14T17:37:53Z 2025-07-14T17:37:53Z |
| dc.format.none.fl_str_mv | 11 p application/pdf |
| dc.identifier.none.fl_str_mv | López Radcenco, A, Adrien Delgado, M, Ruprechter Schölderle, G, Torres, E, Meikle, A y Moyna, G. Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics. Dairy. [en línea] 2021 2021, 2, 356-366 https://hdl.handle.net/20.500.12008/50598 https://doi.org/10.3390/dairy2030028 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Dairy, 2021, 2, 356-366 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | VACAS LECHERAS PRODUCCION LECHERA PARTO ENFERMEDADES TRANSTORNOS METABOLICOS |
| dc.title.none.fl_str_mv | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The metabolic alterations associated with the increase in milk production make the transition period critical to the health of dairy cows, usually leading to a higher incidence of disease in periparturient animals. In this manuscript, we describe the use of NMR-based untargeted metabolomics to follow how these changes impact the serum metabolome in a group of 28 transition dairy cows with no initial clinical diseases. Principal component analysis (PCA) of serum 1H NMR data from four weeks before calving to 8 weeks after parturition allowed us to clearly identify four stages during the transition period. Pairwise comparisons using orthogonal partial least square discriminant analysis (OPLS-DA) and univariate data analysis led to the identification of 18 metabolites that varied significantly through these stages. Species such as acetate, betaine, and creatine are observed early after calving, while other markers of metabolic stress, including acetone, β-hydroxybutyrate (BHB), and choline, accumulate significantly at the height of milk production. Furthermore, marked variations in the levels of lactate, allantoin, alanine, and other amino acids reveal the activation of different gluconeogenic pathways following parturition. Concomitant with a return to homeostasis, a gradual normalization of the serum metabolome occurs 8 weeks after calving. Correlations of metabolite levels with dietary and metabolic adaptations based on animal parity could also be identified. Overall, these results show that NMR-based chemometric methods are ideally suited to monitor manifestations of metabolic diseases throughout the transition period and to assess the impact of nutritional management schemes on the metabolism of dairy cows. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_a82dc6a7f82395db48add91c3cfd1a9c |
| identifier_str_mv | López Radcenco, A, Adrien Delgado, M, Ruprechter Schölderle, G, Torres, E, Meikle, A y Moyna, G. Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics. Dairy. [en línea] 2021 2021, 2, 356-366 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50598 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted MetabolomicsLópez Radcenco, AndrésAdrien Delgado, María de LourdesRuprechter Schölderle, Gretel CristinaTorres, Elena deMeikle, AnaMoyna, GuillermoVACAS LECHERASPRODUCCION LECHERAPARTOENFERMEDADESTRANSTORNOS METABOLICOSThe metabolic alterations associated with the increase in milk production make the transition period critical to the health of dairy cows, usually leading to a higher incidence of disease in periparturient animals. In this manuscript, we describe the use of NMR-based untargeted metabolomics to follow how these changes impact the serum metabolome in a group of 28 transition dairy cows with no initial clinical diseases. Principal component analysis (PCA) of serum 1H NMR data from four weeks before calving to 8 weeks after parturition allowed us to clearly identify four stages during the transition period. Pairwise comparisons using orthogonal partial least square discriminant analysis (OPLS-DA) and univariate data analysis led to the identification of 18 metabolites that varied significantly through these stages. Species such as acetate, betaine, and creatine are observed early after calving, while other markers of metabolic stress, including acetone, β-hydroxybutyrate (BHB), and choline, accumulate significantly at the height of milk production. Furthermore, marked variations in the levels of lactate, allantoin, alanine, and other amino acids reveal the activation of different gluconeogenic pathways following parturition. Concomitant with a return to homeostasis, a gradual normalization of the serum metabolome occurs 8 weeks after calving. Correlations of metabolite levels with dietary and metabolic adaptations based on animal parity could also be identified. Overall, these results show that NMR-based chemometric methods are ideally suited to monitor manifestations of metabolic diseases throughout the transition period and to assess the impact of nutritional management schemes on the metabolism of dairy cows.López Radcenco Andrés, Universidad de la República (Uruguay). CENUR Litoral Norte. Departamento de Química del LitoralAdrien Delgado María de Lourdes, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Salud en los Sistemas PecuariosRuprechter Schölderle Gretel Cristina, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Endocrinología y Metabolismo AnimalTorres Elena de, Universidad de la República (Uruguay). Facultad de Veterinaria. Campo Experimental 2Meikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Endocrinología y Metabolismo AnimalMoyna Guillermo, Universidad de la República (Uruguay). CENUR Litoral Norte. Departamento de Química del Litoral2025-07-14T17:37:53Z2025-07-14T17:37:53Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 papplication/pdfLópez Radcenco, A, Adrien Delgado, M, Ruprechter Schölderle, G, Torres, E, Meikle, A y Moyna, G. Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics. Dairy. [en línea] 2021 2021, 2, 356-366https://hdl.handle.net/20.500.12008/50598https://doi.org/10.3390/dairy2030028reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengDairy, 2021, 2, 356-366Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/505982026-04-14T10:35:06Z |
| spellingShingle | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics López Radcenco, Andrés VACAS LECHERAS PRODUCCION LECHERA PARTO ENFERMEDADES TRANSTORNOS METABOLICOS |
| status_str | publishedVersion |
| title | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| title_full | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| title_fullStr | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| title_full_unstemmed | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| title_short | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| title_sort | Monitoring the Transition Period in Dairy Cows through 1H NMR-Based Untargeted Metabolomics |
| topic | VACAS LECHERAS PRODUCCION LECHERA PARTO ENFERMEDADES TRANSTORNOS METABOLICOS |
| url | https://hdl.handle.net/20.500.12008/50598 https://doi.org/10.3390/dairy2030028 |