Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants
The development of the whole genome sequencing (WGS) has been possible characterized isolates of Listeria recovered form listeriosis cases in ruminants, in an attempt to increase the knowledge about the disease, the involved strains and also the possible relationship with isolates from listeriosis i...
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| Language: | English |
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2025
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| Online Access: | https://hdl.handle.net/20.500.12381/5348 |
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| _version_ | 1868890156506808320 |
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| author | Matto, Carolina |
| author2 | Mota, Maria Ines D'Alessandro, Bruno Braga, Valeria Papa, Romina Betancor, Laura Varela, Gustavo |
| author2_role | author author author author author author |
| author_browse | Betancor, Laura Braga, Valeria D'Alessandro, Bruno Matto, Carolina Mota, Maria Ines Papa, Romina Varela, Gustavo |
| author_facet | Matto, Carolina Mota, Maria Ines D'Alessandro, Bruno Braga, Valeria Papa, Romina Betancor, Laura Varela, Gustavo |
| author_role | author |
| collection | REDI |
| dc.creator.none.fl_str_mv | Matto, Carolina Mota, Maria Ines D'Alessandro, Bruno Braga, Valeria Papa, Romina Betancor, Laura Varela, Gustavo |
| dc.date.none.fl_str_mv | 2025-12-24T13:03:15Z 2025 |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/5348 FSSA_1_2019_1_160057 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer Nature |
| dc.rights.none.fl_str_mv | Acceso embargado info:eu-repo/semantics/embargoedAccess Reconocimiento 4.0 Internacional. (CC BY) 2027-07-01 2027-07-01 |
| dc.source.none.fl_str_mv | Veterinary Research Communications reponame:REDI instname:Agencia Nacional de Investigación e Innovación instacron:Agencia Nacional de Investigación e Innovación |
| dc.subject.none.fl_str_mv | Cattle Clonal complex Neurolisteriosis Ciencias Agrícolas Ciencias Veterinarias Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología |
| dc.title.none.fl_str_mv | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Borrador info:eu-repo/semantics/draft |
| description | The development of the whole genome sequencing (WGS) has been possible characterized isolates of Listeria recovered form listeriosis cases in ruminants, in an attempt to increase the knowledge about the disease, the involved strains and also the possible relationship with isolates from listeriosis in humans. However, the information reported and collected in databases come from Europe and North America. We analyzed 16 L. monocytogenes and 4 L. innocua isolates recovered from listeriosis in cattle, sheep and a goat from Uruguay through multilocus sequence typing (MLST), core genome multilocus sequence typing (cgMLST) and virulence gene profile analysis. L. monocytogenes lineage I predominated over lineage II (14 vs 2 isolates, respectively). There were 9 different sequence types (STs), being ST1 the most common (6/16, 37.5%) and 7 different clonal complexes (CC’s), in which CC1 was the most frequent (7/16, 43.75%). cgMLST identified 15 different CT’s, showing that there was diversity of strains causing disease. All L. monocytogenes harbored the main virulence factors, 12 isolates possess also a full length LIPI-3 and, two of them LIPI-4. The four L. innocua isolates were grouped into 3 different CC’s, 29 virulence-associated genes were identified, as well as a full LIPI-4. Despite our dataset is small in comparison with other reports, it is the first that analyzed Listeria from South American ruminants, contributing to enhance the knowledge about the disease and characteristics of the involved strains. |
| eu_rights_str_mv | embargoedAccess |
| format | article |
| id | anni_a5f6ca9b2a87e4c2d0c2fde2b909318e |
| identifier_str_mv | FSSA_1_2019_1_160057 |
| instacron_str | Agencia Nacional de Investigación e Innovación |
| institution | Agencia Nacional de Investigación e Innovación |
| instname_str | Agencia Nacional de Investigación e Innovación |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/5348 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| publisher.none.fl_str_mv | Springer Nature |
| reponame_str | REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso embargado Reconocimiento 4.0 Internacional. (CC BY) 2027-07-01 |
| spelling | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminantsMatto, CarolinaMota, Maria InesD'Alessandro, BrunoBraga, ValeriaPapa, RominaBetancor, LauraVarela, GustavoCattleClonal complexNeurolisteriosisCiencias AgrícolasCiencias VeterinariasCiencias Naturales y ExactasCiencias BiológicasBiología Celular, MicrobiologíaThe development of the whole genome sequencing (WGS) has been possible characterized isolates of Listeria recovered form listeriosis cases in ruminants, in an attempt to increase the knowledge about the disease, the involved strains and also the possible relationship with isolates from listeriosis in humans. However, the information reported and collected in databases come from Europe and North America. We analyzed 16 L. monocytogenes and 4 L. innocua isolates recovered from listeriosis in cattle, sheep and a goat from Uruguay through multilocus sequence typing (MLST), core genome multilocus sequence typing (cgMLST) and virulence gene profile analysis. L. monocytogenes lineage I predominated over lineage II (14 vs 2 isolates, respectively). There were 9 different sequence types (STs), being ST1 the most common (6/16, 37.5%) and 7 different clonal complexes (CC’s), in which CC1 was the most frequent (7/16, 43.75%). cgMLST identified 15 different CT’s, showing that there was diversity of strains causing disease. All L. monocytogenes harbored the main virulence factors, 12 isolates possess also a full length LIPI-3 and, two of them LIPI-4. The four L. innocua isolates were grouped into 3 different CC’s, 29 virulence-associated genes were identified, as well as a full LIPI-4. Despite our dataset is small in comparison with other reports, it is the first that analyzed Listeria from South American ruminants, contributing to enhance the knowledge about the disease and characteristics of the involved strains.Agencia Nacional de Investigación e InnovaciónSpringer Nature2025-12-24T13:03:15Z2025Artículoinfo:eu-repo/semantics/articleBorradorinfo:eu-repo/semantics/drafthttps://hdl.handle.net/20.500.12381/5348FSSA_1_2019_1_160057Veterinary Research Communicationsreponame:REDIinstname:Agencia Nacional de Investigación e Innovacióninstacron:Agencia Nacional de Investigación e InnovaciónengAcceso embargadoinfo:eu-repo/semantics/embargoedAccessReconocimiento 4.0 Internacional. (CC BY)2027-07-01El trabajo se encuentra en redacción, luego sera enviado a revista arbitrada, por lo cual no es posible se divulgación hasta entonces. Aclaración: Según la política de la revista se puede publicar con un embargo de 12 meses.2027-07-01oai:redi.anii.org.uy:20.500.12381/53482026-06-16T05:01:28Z |
| spellingShingle | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants Matto, Carolina Cattle Clonal complex Neurolisteriosis Ciencias Agrícolas Ciencias Veterinarias Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología |
| status_str | draft |
| title | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| title_full | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| title_fullStr | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| title_full_unstemmed | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| title_short | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| title_sort | Genetic diversity and virulence profiles of Listeria monocytogenes and L. innocua strains isolated in Uruguay from clinical cases in ruminants |
| topic | Cattle Clonal complex Neurolisteriosis Ciencias Agrícolas Ciencias Veterinarias Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología |
| url | https://hdl.handle.net/20.500.12381/5348 |