Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy
The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin rece...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , , , , |
| Formato: | article |
| Lenguaje: | español |
| Publicado: |
2004
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12008/51548 https://doi.org/10.3168/jds.S0022-0302(04)73463-3 |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1868890152945844224 |
|---|---|
| author | Guzeloglu, A. |
| author2 | Bilby, T.R. Meikle, Ana Kamimura, S. Kowalski, A. Michel, F. MacLaren, L.A. Thatcher, W.W. |
| author2_role | author author author author author author author |
| author_browse | Bilby, T.R. Guzeloglu, A. Kamimura, S. Kowalski, A. MacLaren, L.A. Meikle, Ana Michel, F. Thatcher, W.W. |
| author_facet | Guzeloglu, A. Bilby, T.R. Meikle, Ana Kamimura, S. Kowalski, A. Michel, F. MacLaren, L.A. Thatcher, W.W. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Guzeloglu A., University of Florida (Gainesville, Florida). Department of Animal Sciences Bilby T.R., University of Florida (Gainesville, Florida). Department of Animal Sciences Meikle Ana, University of Uruguay (Uruguay). Faculty of Veterinary Medicine. Department of Biochemistry Kamimura S., Kagoshima University (Kagoshima, Japan). Faculty of Agriculture. Department of Veterinary Medicine Kowalski A., Universidad Centroccidental Lisandro Alvarado (Barquisimeto, Lara, Venezuela). Dept. Animal Production. College of Agriculture Michel F., University of Florida (Gainesville, Florida). Department of Animal Sciences MacLaren L.A., Nova Scotia Agricultural College (Truro, Canada). Department of Plant and Animal Sciences Thatcher W.W., University of Florida (Gainesville, Florida). Department of Animal Sciences |
| dc.creator.none.fl_str_mv | Guzeloglu, A. Bilby, T.R. Meikle, Ana Kamimura, S. Kowalski, A. Michel, F. MacLaren, L.A. Thatcher, W.W. |
| dc.date.none.fl_str_mv | 2004 2025-09-10T14:17:38Z 2025-09-10T14:17:38Z |
| dc.format.none.fl_str_mv | 12 p application/pdf |
| dc.identifier.none.fl_str_mv | Guzeloglu, A, Bilby, T, Meikle, A, Kamimura, S, Kowalski, A, Michel, F, MacLaren, L y Thatcher, W. Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy. Journal of Dairy Science. [en línea] 2004, 87(10), 3268–3279 https://hdl.handle.net/20.500.12008/51548 https://doi.org/10.3168/jds.S0022-0302(04)73463-3 |
| dc.language.none.fl_str_mv | es spa |
| dc.relation.none.fl_str_mv | Journal of Dairy Science, 2004, 87(10), 3268–3279 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | BOVINOS VACAS LECHERAS GESTACION ENDOMETRIO SOMATOTROPINA BOVINA |
| dc.title.none.fl_str_mv | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bSTtreated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor α (ERα) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERα protein, and bST stimulated an increase in ERα protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERα protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P cows than in P cows. In the stromal layer of the endometrium, bST decreased PR protein abundance in C and P cows. The PGHS-2 protein was localized exclusively in the luminal epithelium cells of endometrium and was increased in P cows. In conclusion, distinctly different mRNA and protein responses were detected between C and P cows related to prostaglandin biosynthesis, and bST-induced changes may potentially impact mechanisms associated with maintenance of pregnancy in nonlactating cows. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_a2daef1829b3d2f429bd43846f22367c |
| identifier_str_mv | Guzeloglu, A, Bilby, T, Meikle, A, Kamimura, S, Kowalski, A, Michel, F, MacLaren, L y Thatcher, W. Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy. Journal of Dairy Science. [en línea] 2004, 87(10), 3268–3279 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | spa |
| language_invalid_str_mv | es |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/51548 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of PregnancyGuzeloglu, A.Bilby, T.R.Meikle, AnaKamimura, S.Kowalski, A.Michel, F.MacLaren, L.A.Thatcher, W.W.BOVINOSVACAS LECHERASGESTACIONENDOMETRIOSOMATOTROPINA BOVINAThe objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bSTtreated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor α (ERα) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERα protein, and bST stimulated an increase in ERα protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERα protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P cows than in P cows. In the stromal layer of the endometrium, bST decreased PR protein abundance in C and P cows. The PGHS-2 protein was localized exclusively in the luminal epithelium cells of endometrium and was increased in P cows. In conclusion, distinctly different mRNA and protein responses were detected between C and P cows related to prostaglandin biosynthesis, and bST-induced changes may potentially impact mechanisms associated with maintenance of pregnancy in nonlactating cows.Guzeloglu A., University of Florida (Gainesville, Florida). Department of Animal SciencesBilby T.R., University of Florida (Gainesville, Florida). Department of Animal SciencesMeikle Ana, University of Uruguay (Uruguay). Faculty of Veterinary Medicine. Department of BiochemistryKamimura S., Kagoshima University (Kagoshima, Japan). Faculty of Agriculture. Department of Veterinary MedicineKowalski A., Universidad Centroccidental Lisandro Alvarado (Barquisimeto, Lara, Venezuela). Dept. Animal Production. College of AgricultureMichel F., University of Florida (Gainesville, Florida). Department of Animal SciencesMacLaren L.A., Nova Scotia Agricultural College (Truro, Canada). Department of Plant and Animal SciencesThatcher W.W., University of Florida (Gainesville, Florida). Department of Animal Sciences2025-09-10T14:17:38Z2025-09-10T14:17:38Z2004Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 papplication/pdfGuzeloglu, A, Bilby, T, Meikle, A, Kamimura, S, Kowalski, A, Michel, F, MacLaren, L y Thatcher, W. Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy. Journal of Dairy Science. [en línea] 2004, 87(10), 3268–3279https://hdl.handle.net/20.500.12008/51548https://doi.org/10.3168/jds.S0022-0302(04)73463-3reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaesspaJournal of Dairy Science, 2004, 87(10), 3268–3279Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/515482026-04-14T10:35:08Z |
| spellingShingle | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy Guzeloglu, A. BOVINOS VACAS LECHERAS GESTACION ENDOMETRIO SOMATOTROPINA BOVINA |
| status_str | publishedVersion |
| title | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| title_full | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| title_fullStr | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| title_full_unstemmed | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| title_short | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| title_sort | Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy |
| topic | BOVINOS VACAS LECHERAS GESTACION ENDOMETRIO SOMATOTROPINA BOVINA |
| url | https://hdl.handle.net/20.500.12008/51548 https://doi.org/10.3168/jds.S0022-0302(04)73463-3 |