Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth.
The toxic cyanobacterium Microcystis causes worldwide health concerns, being requently found in freshwater and estuarine ecosystems. Under natural conditions, Microcystis spp. show a colonial lifestyle involving a phycosphere populated by a highly diverse associated microbiome. In a previous study,...
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| Format: | article |
| Language: | English |
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2025
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| Online Access: | https://hdl.handle.net/20.500.12381/5343 |
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| _version_ | 1868890147486957568 |
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| author | Croci, Carolina |
| author2 | Martínez de la Escalera, Gabriela Kruk, Carla Segura, Angel Deus Alvarez, Susana Piccini, Claudia |
| author2_role | author author author author author |
| author_browse | Croci, Carolina Deus Alvarez, Susana Kruk, Carla Martínez de la Escalera, Gabriela Piccini, Claudia Segura, Angel |
| author_facet | Croci, Carolina Martínez de la Escalera, Gabriela Kruk, Carla Segura, Angel Deus Alvarez, Susana Piccini, Claudia |
| author_role | author |
| collection | IIBCE en REDI |
| dc.creator.none.fl_str_mv | Croci, Carolina Martínez de la Escalera, Gabriela Kruk, Carla Segura, Angel Deus Alvarez, Susana Piccini, Claudia |
| dc.date.none.fl_str_mv | 2025-12-17T17:02:01Z 2025-12-17T17:02:01Z 2025-04-04 |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/5343 FCE_1_2019_1_156308 10.7717/peerj.19149 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | PeerJ |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND) |
| dc.source.none.fl_str_mv | reponame:IIBCE en REDI instname:Instituto de Investigaciones Biológicas Clemente Estable instacron:Instituto de Investigaciones Biológicas Clemente Estable |
| dc.subject.none.fl_str_mv | Microcystis phycosphere multispecies biofilm colony development Ciencias Naturales y Exactas Ciencias Biológicas Ecología |
| dc.title.none.fl_str_mv | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | The toxic cyanobacterium Microcystis causes worldwide health concerns, being requently found in freshwater and estuarine ecosystems. Under natural conditions, Microcystis spp. show a colonial lifestyle involving a phycosphere populated by a highly diverse associated microbiome. In a previous study, we have proposed that colony formation and growth may be achieved through mechanisms of multispecies bacterial biofilm formation. Starting with single-cells, specific bacteria would be recruited from the environment to attach and create a buoyant biofilm or colony. This progression from a few single cells to large colonies would encompass the growth of the Microcystis community and bloom formation. In order to test this, we applied 16S rDNA metabarcoding to evaluate the changes in bacterial community structure (gDNA) and its active portion (cDNA) between different sample sizes obtained from a Microcystis bloom. Bloom sample was sieved by size, from one or a few cells (U fraction) to large colonies (maximum linear dimension ≥ 150 μm; L fraction), including small (20–60 μm, S fraction) and medium size (60–150 μm, M fraction) colonies. We found that gDNA- and cDNA-based bacterial assemblages significantly differed mostly due to the presence of different taxa that became active among the different sizes. The compositional variations in the communities between the assessed sample sizes were mainly attributed to turnover. From U to M fractions the turnover was a result of selection processes, while between M and L fractions stochastic processes were likely responsible for the changes. The results suggest that colony formation and growth are a consequence of mechanisms accounting for recruitment and selection of specific bacterial groups, which activate or stop growing through the different phases of the biofilm formation. When the final phase (L fraction colonies) is reached the colonies start to disaggregate (bloom decay), few cells or single cells are released and they can start new biofilms when conditions are suitable (bloom development). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_9eb92a05f7408b30e06b7cdce5f46d2e |
| identifier_str_mv | FCE_1_2019_1_156308 10.7717/peerj.19149 |
| instacron_str | Instituto de Investigaciones Biológicas Clemente Estable |
| institution | Instituto de Investigaciones Biológicas Clemente Estable |
| instname_str | Instituto de Investigaciones Biológicas Clemente Estable |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/5343 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| publisher.none.fl_str_mv | PeerJ |
| reponame_str | IIBCE en REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND) |
| spelling | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth.Croci, CarolinaMartínez de la Escalera, GabrielaKruk, CarlaSegura, AngelDeus Alvarez, SusanaPiccini, ClaudiaMicrocystisphycospheremultispecies biofilmcolony developmentCiencias Naturales y ExactasCiencias BiológicasEcologíaThe toxic cyanobacterium Microcystis causes worldwide health concerns, being requently found in freshwater and estuarine ecosystems. Under natural conditions, Microcystis spp. show a colonial lifestyle involving a phycosphere populated by a highly diverse associated microbiome. In a previous study, we have proposed that colony formation and growth may be achieved through mechanisms of multispecies bacterial biofilm formation. Starting with single-cells, specific bacteria would be recruited from the environment to attach and create a buoyant biofilm or colony. This progression from a few single cells to large colonies would encompass the growth of the Microcystis community and bloom formation. In order to test this, we applied 16S rDNA metabarcoding to evaluate the changes in bacterial community structure (gDNA) and its active portion (cDNA) between different sample sizes obtained from a Microcystis bloom. Bloom sample was sieved by size, from one or a few cells (U fraction) to large colonies (maximum linear dimension ≥ 150 μm; L fraction), including small (20–60 μm, S fraction) and medium size (60–150 μm, M fraction) colonies. We found that gDNA- and cDNA-based bacterial assemblages significantly differed mostly due to the presence of different taxa that became active among the different sizes. The compositional variations in the communities between the assessed sample sizes were mainly attributed to turnover. From U to M fractions the turnover was a result of selection processes, while between M and L fractions stochastic processes were likely responsible for the changes. The results suggest that colony formation and growth are a consequence of mechanisms accounting for recruitment and selection of specific bacterial groups, which activate or stop growing through the different phases of the biofilm formation. When the final phase (L fraction colonies) is reached the colonies start to disaggregate (bloom decay), few cells or single cells are released and they can start new biofilms when conditions are suitable (bloom development).Agencia Nacional de Investigación e InnovaciónPeerJ2025-12-17T17:02:01Z2025-12-17T17:02:01Z2025-04-04Artículoinfo:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/20.500.12381/5343FCE_1_2019_1_15630810.7717/peerj.19149reponame:IIBCE en REDIinstname:Instituto de Investigaciones Biológicas Clemente Estableinstacron:Instituto de Investigaciones Biológicas Clemente EstableengAcceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND)oai:redi.anii.org.uy:20.500.12381/53432026-06-16T05:25:36Z |
| spellingShingle | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. Croci, Carolina Microcystis phycosphere multispecies biofilm colony development Ciencias Naturales y Exactas Ciencias Biológicas Ecología |
| status_str | publishedVersion |
| title | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| title_full | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| title_fullStr | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| title_full_unstemmed | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| title_short | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| title_sort | Selective enrichment of active bacterial taxa in the Microcystis associated microbiome during colony growth. |
| topic | Microcystis phycosphere multispecies biofilm colony development Ciencias Naturales y Exactas Ciencias Biológicas Ecología |
| url | https://hdl.handle.net/20.500.12381/5343 |