The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia
Giardia is an intestinal parasite that belongs to the earliest diverging branch of the eukaryotic lineage of descent. Giardia undergoes adaptation for survival outside the host’s intestine by differentiating into infective cysts. Encystation involves the synthesis and transport of cyst wall constitu...
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| Format: | article |
| Language: | English |
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2002
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| Online Access: | https://hdl.handle.net/20.500.12008/51261 |
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| _version_ | 1868890145720107008 |
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| author | Touz, María C. |
| author2 | Nores, María J. Slavin, Ileana Carmona, Carlos Conrad, John T. Mowatt, Michael R. Nash, Theodore E. Coronel, Carlos E. Luján, Hugo D. |
| author2_role | author author author author author author author author |
| author_browse | Carmona, Carlos Conrad, John T. Coronel, Carlos E. Luján, Hugo D. Mowatt, Michael R. Nash, Theodore E. Nores, María J. Slavin, Ileana Touz, María C. |
| author_facet | Touz, María C. Nores, María J. Slavin, Ileana Carmona, Carlos Conrad, John T. Mowatt, Michael R. Nash, Theodore E. Coronel, Carlos E. Luján, Hugo D. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Touz María C., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular Nores María J., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular Slavin Ileana, Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular Carmona Carlos, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Laboratorio de Biología Parasitaria Conrad John T., National Institutes of Health (Estados Unidos). Laboratory of Parasitic Diseases Mowatt Michael R., National Institutes of Health (Estados Unidos). Laboratory of Parasitic Diseases Nash Theodore E., National Institutes of Health (Estados Unidos). Laboratory of Parasitic Diseases Coronel Carlos E., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular Luján Hugo D., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular |
| dc.creator.none.fl_str_mv | Touz, María C. Nores, María J. Slavin, Ileana Carmona, Carlos Conrad, John T. Mowatt, Michael R. Nash, Theodore E. Coronel, Carlos E. Luján, Hugo D. |
| dc.date.none.fl_str_mv | 2002 2025-08-26T19:39:50Z 2025-08-26T19:39:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | TOUZ, MC., NORES, MJ., SLAVIN, I., y otros. The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia. J. Biol. Chem [en línea] 2002, 277(10). DOI: 10.1074/jbc.M110250200 https://hdl.handle.net/20.500.12008/51261 10.1074/jbc.M110250200 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | J. Biol. Chem. 277(10), 2002 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | CYSTEINE PROTEASES CYSTS EUKARYOTA GIARDIA LAMBLIA |
| dc.title.none.fl_str_mv | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Giardia is an intestinal parasite that belongs to the earliest diverging branch of the eukaryotic lineage of descent. Giardia undergoes adaptation for survival outside the host’s intestine by differentiating into infective cysts. Encystation involves the synthesis and transport of cyst wall constituents to the plasma membrane for release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression of these proteins is coordinately induced, and both concentrated within encystation-specific secretory vesicles before their extracellular polymerization. Although highly similar to each other at the amino terminus, CWP2 includes a COOHterminal 121-amino acid extension. Here, we show that this extension, rich in basic residues, is cleaved from CWP2 before cyst wall formation by an intracellular cysteine proteinase activity, which is induced during encystation like CWPs. Specific inhibitors prevent release of cyst wall materials, abolishing cyst wall formation. We also report the purification, cloning, and characterization of the encystation-specific cysteine proteinase responsible for the proteolytic processing of CWP2, which is homologue to lysosomal cathepsin C. Encystation-specific cysteine proteinase ESCP possesses unique characteristics compared with cathepsins from higher eukaryotes, such as a transmembrane domain and a short cytoplasmic tail. These features make this enzyme the most divergent cathepsin C identified to date and provide new insights regarding cyst wall formation in Giardia. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_9cd5c018cc1692d586edbda14edfaade |
| identifier_str_mv | TOUZ, MC., NORES, MJ., SLAVIN, I., y otros. The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia. J. Biol. Chem [en línea] 2002, 277(10). DOI: 10.1074/jbc.M110250200 10.1074/jbc.M110250200 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/51261 |
| publishDate | 2002 |
| publishDateSort | 2002 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lambliaTouz, María C.Nores, María J.Slavin, IleanaCarmona, CarlosConrad, John T.Mowatt, Michael R.Nash, Theodore E.Coronel, Carlos E.Luján, Hugo D.CYSTEINE PROTEASESCYSTSEUKARYOTAGIARDIA LAMBLIAGiardia is an intestinal parasite that belongs to the earliest diverging branch of the eukaryotic lineage of descent. Giardia undergoes adaptation for survival outside the host’s intestine by differentiating into infective cysts. Encystation involves the synthesis and transport of cyst wall constituents to the plasma membrane for release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression of these proteins is coordinately induced, and both concentrated within encystation-specific secretory vesicles before their extracellular polymerization. Although highly similar to each other at the amino terminus, CWP2 includes a COOHterminal 121-amino acid extension. Here, we show that this extension, rich in basic residues, is cleaved from CWP2 before cyst wall formation by an intracellular cysteine proteinase activity, which is induced during encystation like CWPs. Specific inhibitors prevent release of cyst wall materials, abolishing cyst wall formation. We also report the purification, cloning, and characterization of the encystation-specific cysteine proteinase responsible for the proteolytic processing of CWP2, which is homologue to lysosomal cathepsin C. Encystation-specific cysteine proteinase ESCP possesses unique characteristics compared with cathepsins from higher eukaryotes, such as a transmembrane domain and a short cytoplasmic tail. These features make this enzyme the most divergent cathepsin C identified to date and provide new insights regarding cyst wall formation in Giardia.Touz María C., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología MolecularNores María J., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología MolecularSlavin Ileana, Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología MolecularCarmona Carlos, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Laboratorio de Biología ParasitariaConrad John T., National Institutes of Health (Estados Unidos). Laboratory of Parasitic DiseasesMowatt Michael R., National Institutes of Health (Estados Unidos). Laboratory of Parasitic DiseasesNash Theodore E., National Institutes of Health (Estados Unidos). Laboratory of Parasitic DiseasesCoronel Carlos E., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología MolecularLuján Hugo D., Universidad Nacional de Córdoba (Argentina). Facultad de Ciencias Medicas. Cátedra de Bioquímica y Biología Molecular2025-08-26T19:39:50Z2025-08-26T19:39:50Z2002Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfTOUZ, MC., NORES, MJ., SLAVIN, I., y otros. The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia. J. Biol. Chem [en línea] 2002, 277(10). DOI: 10.1074/jbc.M110250200https://hdl.handle.net/20.500.12008/5126110.1074/jbc.M110250200reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJ. Biol. Chem. 277(10), 2002Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/512612026-04-14T10:37:05Z |
| spellingShingle | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia Touz, María C. CYSTEINE PROTEASES CYSTS EUKARYOTA GIARDIA LAMBLIA |
| status_str | publishedVersion |
| title | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| title_full | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| title_fullStr | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| title_full_unstemmed | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| title_short | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| title_sort | The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia |
| topic | CYSTEINE PROTEASES CYSTS EUKARYOTA GIARDIA LAMBLIA |
| url | https://hdl.handle.net/20.500.12008/51261 |