SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse
SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/38147 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890145073135616 |
|---|---|
| author | Capoano Bevilacqua, Carlos Adrián |
| author2 | Ortiz-Laquintana, Luis Adrián Rodríguez-Casuriaga, Rosana Schlapp, Geraldine Meikle, María Noel Mulet, Ana Paula Crispo, Martina Benavente, Ricardo Geisinger Wschebor, Adriana |
| author2_role | author author author author author author author author |
| author_browse | Benavente, Ricardo Capoano Bevilacqua, Carlos Adrián Crispo, Martina Geisinger Wschebor, Adriana Meikle, María Noel Mulet, Ana Paula Ortiz-Laquintana, Luis Adrián Rodríguez-Casuriaga, Rosana Schlapp, Geraldine |
| author_facet | Capoano Bevilacqua, Carlos Adrián Ortiz-Laquintana, Luis Adrián Rodríguez-Casuriaga, Rosana Schlapp, Geraldine Meikle, María Noel Mulet, Ana Paula Crispo, Martina Benavente, Ricardo Geisinger Wschebor, Adriana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Capoano Bevilacqua Carlos Adrián, IIBCE Ortiz-Laquintana Luis Adrián, IIBCE Rodríguez-Casuriaga Rosana, IIBCE Schlapp Geraldine, Instituto Pasteur (Montevideo) Meikle María Noel, Instituto Pasteur (Montevideo) Mulet Ana Paula, Instituto Pasteur (Montevideo) Crispo Martina, Instituto Pasteur (Montevideo) Benavente Ricardo Geisinger Wschebor Adriana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. |
| dc.creator.none.fl_str_mv | Capoano Bevilacqua, Carlos Adrián Ortiz-Laquintana, Luis Adrián Rodríguez-Casuriaga, Rosana Schlapp, Geraldine Meikle, María Noel Mulet, Ana Paula Crispo, Martina Benavente, Ricardo Geisinger Wschebor, Adriana |
| dc.date.none.fl_str_mv | 2021 2023-07-13T15:24:15Z 2023-07-13T15:24:15Z |
| dc.format.none.fl_str_mv | 18 h application/pdf |
| dc.identifier.none.fl_str_mv | Capoano Bevilacqua, C, Ortiz-Laquintana, L, Rodríguez-Casuriaga, R, [y otros autores]. "SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse". PLoS ONE. [en línea] 2021, 16(5): e0251028. 18 h. 1932-6203 https://hdl.handle.net/20.500.12008/38147 10.1371/journal.pone.0251028 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | PloS ONE |
| dc.relation.none.fl_str_mv | PLoS ONE, 2021, 16(5): e0251028 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | SPATS1 ESPERMATOGENESIS FERTILIDAD |
| dc.title.none.fl_str_mv | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_9bd1f723f2aa8a2e642c317bcc5d1e67 |
| identifier_str_mv | Capoano Bevilacqua, C, Ortiz-Laquintana, L, Rodríguez-Casuriaga, R, [y otros autores]. "SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse". PLoS ONE. [en línea] 2021, 16(5): e0251028. 18 h. 1932-6203 10.1371/journal.pone.0251028 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/38147 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher.none.fl_str_mv | PloS ONE |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouseCapoano Bevilacqua, Carlos AdriánOrtiz-Laquintana, Luis AdriánRodríguez-Casuriaga, RosanaSchlapp, GeraldineMeikle, María NoelMulet, Ana PaulaCrispo, MartinaBenavente, RicardoGeisinger Wschebor, AdrianaSPATS1ESPERMATOGENESISFERTILIDADSPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.PloS ONECapoano Bevilacqua Carlos Adrián, IIBCEOrtiz-Laquintana Luis Adrián, IIBCERodríguez-Casuriaga Rosana, IIBCESchlapp Geraldine, Instituto Pasteur (Montevideo)Meikle María Noel, Instituto Pasteur (Montevideo)Mulet Ana Paula, Instituto Pasteur (Montevideo)Crispo Martina, Instituto Pasteur (Montevideo)Benavente RicardoGeisinger Wschebor Adriana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.2023-07-13T15:24:15Z2023-07-13T15:24:15Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 happlication/pdfCapoano Bevilacqua, C, Ortiz-Laquintana, L, Rodríguez-Casuriaga, R, [y otros autores]. "SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse". PLoS ONE. [en línea] 2021, 16(5): e0251028. 18 h.1932-6203https://hdl.handle.net/20.500.12008/3814710.1371/journal.pone.0251028reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengPLoS ONE, 2021, 16(5): e0251028Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/381472026-04-14T10:10:07Z |
| spellingShingle | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse Capoano Bevilacqua, Carlos Adrián SPATS1 ESPERMATOGENESIS FERTILIDAD |
| status_str | publishedVersion |
| title | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| title_full | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| title_fullStr | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| title_full_unstemmed | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| title_short | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| title_sort | SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse |
| topic | SPATS1 ESPERMATOGENESIS FERTILIDAD |
| url | https://hdl.handle.net/20.500.12008/38147 |