A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity
Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/22012 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890140906094592 |
|---|---|
| author | Noya, Verónica |
| author2 | Brossard Barbosa, Natalie Rodríguez, Ernesto Dergan-Dylon, L. S. Carmona García, Carlos Rabinovich, G. A. Freire, Teresa |
| author2_role | author author author author author author |
| author_browse | Brossard Barbosa, Natalie Carmona García, Carlos Dergan-Dylon, L. S. Freire, Teresa Noya, Verónica Rabinovich, G. A. Rodríguez, Ernesto |
| author_facet | Noya, Verónica Brossard Barbosa, Natalie Rodríguez, Ernesto Dergan-Dylon, L. S. Carmona García, Carlos Rabinovich, G. A. Freire, Teresa |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Noya, Verónica. Universidad de la República (Uruguay). Facultad de Medicina Brossard, Natalia. Universidad de la República (Uruguay). Facultad de Medicina Rodríguez, Ernesto. Universidad de la República (Uruguay). Facultad de Medicina Carmona, Carlos. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Freire, Teresa. Universidad de la República (Uruguay). Facultad de Medicina. |
| dc.creator.none.fl_str_mv | Noya, Verónica Brossard Barbosa, Natalie Rodríguez, Ernesto Dergan-Dylon, L. S. Carmona García, Carlos Rabinovich, G. A. Freire, Teresa |
| dc.date.none.fl_str_mv | 2017 2019-10-02T22:08:26Z 2019-10-02T22:08:26Z 20190930 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Noya, V.,et al. "A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity". Scientific Reports, 2017, 7, art. nro. 40615.doi: 10.1038/srep40615 2045-2322 https://hdl.handle.net/20.500.12008/22012 10.1038/srep40615 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Nature Publishing Group |
| dc.relation.none.fl_str_mv | Scientific Reports, 2017, 7, art. no. 40615 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC –BY 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Fasciola hepatica Parasites Helminth antibody |
| dc.title.none.fl_str_mv | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN-3 secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-3 secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, Fhmuc-treated DC conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_97362bab07b9e9f3fd7ca281fb2e7673 |
| identifier_str_mv | Noya, V.,et al. "A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity". Scientific Reports, 2017, 7, art. nro. 40615.doi: 10.1038/srep40615 2045-2322 10.1038/srep40615 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/22012 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher.none.fl_str_mv | Nature Publishing Group |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC –BY 4.0) |
| spelling | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activityNoya, VerónicaBrossard Barbosa, NatalieRodríguez, ErnestoDergan-Dylon, L. S.Carmona García, CarlosRabinovich, G. A.Freire, TeresaFasciola hepaticaParasitesHelminth antibodyFasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN-3 secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-3 secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, Fhmuc-treated DC conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies.Nature Publishing GroupNoya, Verónica. Universidad de la República (Uruguay). Facultad de MedicinaBrossard, Natalia. Universidad de la República (Uruguay). Facultad de MedicinaRodríguez, Ernesto. Universidad de la República (Uruguay). Facultad de MedicinaCarmona, Carlos. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFreire, Teresa. Universidad de la República (Uruguay). Facultad de Medicina.2019-10-02T22:08:26Z2019-10-02T22:08:26Z201720190930Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfNoya, V.,et al. "A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity". Scientific Reports, 2017, 7, art. nro. 40615.doi: 10.1038/srep406152045-2322https://hdl.handle.net/20.500.12008/2201210.1038/srep40615reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengScientific Reports, 2017, 7, art. no. 40615Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad De La República. (Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC –BY 4.0)oai:colibri.udelar.edu.uy:20.500.12008/220122026-04-14T10:09:15Z |
| spellingShingle | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity Noya, Verónica Fasciola hepatica Parasites Helminth antibody |
| status_str | publishedVersion |
| title | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_full | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_fullStr | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_full_unstemmed | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_short | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_sort | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| topic | Fasciola hepatica Parasites Helminth antibody |
| url | https://hdl.handle.net/20.500.12008/22012 |