The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy
Abstract: Objectives: Tissue architecture and cell morphology suffer profound alterations during oral cancer and are important markers for its progression and outcome. For precise visualization of tissue architecture in oral cancer, we used confocal microscopy to examine the staining pattern of whea...
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| Format: | article |
| Langue: | anglais |
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2021
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| Accès en ligne: | https://hdl.handle.net/20.500.12008/31424 https://doi.org/10.3390/cells10092466 |
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| author | Silveyra, Estefanía |
| author2 | Bologna-Molina, Ronell Gónzalez-Gónzalez, Rogelio Arocena, Miguel |
| author2_role | author author author |
| author_browse | Arocena, Miguel Bologna-Molina, Ronell Gónzalez-Gónzalez, Rogelio Silveyra, Estefanía |
| author_facet | Silveyra, Estefanía Bologna-Molina, Ronell Gónzalez-Gónzalez, Rogelio Arocena, Miguel |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Silveyra Estefanía, Universidad de la República (Uruguay) Facultad de Odontología Bologna-Molina Ronell, Universidad de la República (Uruguay). Facultad de Odontología Gónzalez-Gónzalez Rogelio, Department of Research, School of Dentistry, Universidad Juárez del Estado de Durango Arocena Miguel, Universidad de la República (Uruguay). Facultad de Odontología. Ministerio de Educación y Cultura. Instituto de Investigaciones Biológicas Clemente Estable |
| dc.creator.none.fl_str_mv | Silveyra, Estefanía Bologna-Molina, Ronell Gónzalez-Gónzalez, Rogelio Arocena, Miguel |
| dc.date.none.fl_str_mv | 2021 2022-05-04T17:20:15Z 2022-05-04T17:20:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | Silveyra, E, Bologna-Molina, R, Gónzalez-Gónzalez, R y otros. "The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy". Cells 2021. [en línea] 2021 10, 2466.https://doi.org/10.3390/cells10092466 https://hdl.handle.net/20.500.12008/31424 https://doi.org/10.3390/cells10092466 |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Cells 2021, 10, 2466. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Oral squamous cell carcinoma Wheat germ agglutinin Tyramide signal amplification Tissue architecture CARCINOMA DE CELULAS ESCAMOSAS AGLUTININAS DEL GERMEN DE TRIGO |
| dc.title.none.fl_str_mv | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Abstract: Objectives: Tissue architecture and cell morphology suffer profound alterations during oral cancer and are important markers for its progression and outcome. For precise visualization of tissue architecture in oral cancer, we used confocal microscopy to examine the staining pattern of wheat germ agglutinin, a lectin that binds membrane glycoproteins, and the staining patterns of structural proteins. Materials and Methods: Paraffin sections of oral squamous cell carcinoma were stained with fluorescently labeled wheat germ agglutinin and with antibodies against structural proteins, which were revealed by immunohistochemistry with tyramide signal amplification. Results: Membrane localization of wheat germ agglutinin was markedly decreased in the basal layers and in regions of tumor invasion, accompanied by cytoplasmic redistribution of E-cadherin, -actin and syndecan-1. Wheat germ agglutinin staining clearly identified tumor clusters within the surrounding stroma, and tumor cells with elongated morphology. Conclusions: Our results suggest that the wheat germ agglutinin staining pattern is indicative of the degree of cell cohesion in oral squamous cell carcinoma, which decreases in basal layers and invasive tumor clusters with more migratory morphologies. Wheat germ agglutinin staining in combination with confocal microscopy could constitute, therefore, a valuable tool for the study of tissue architecture in oral cancer. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_943a4bc1ebb1d7ff2e8392f9ec2ae62f |
| identifier_str_mv | Silveyra, E, Bologna-Molina, R, Gónzalez-Gónzalez, R y otros. "The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy". Cells 2021. [en línea] 2021 10, 2466.https://doi.org/10.3390/cells10092466 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31424 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| spelling | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal MicroscopySilveyra, EstefaníaBologna-Molina, RonellGónzalez-Gónzalez, RogelioArocena, MiguelOral squamous cell carcinomaWheat germ agglutininTyramide signal amplificationTissue architectureCARCINOMA DE CELULAS ESCAMOSASAGLUTININAS DEL GERMEN DE TRIGOAbstract: Objectives: Tissue architecture and cell morphology suffer profound alterations during oral cancer and are important markers for its progression and outcome. For precise visualization of tissue architecture in oral cancer, we used confocal microscopy to examine the staining pattern of wheat germ agglutinin, a lectin that binds membrane glycoproteins, and the staining patterns of structural proteins. Materials and Methods: Paraffin sections of oral squamous cell carcinoma were stained with fluorescently labeled wheat germ agglutinin and with antibodies against structural proteins, which were revealed by immunohistochemistry with tyramide signal amplification. Results: Membrane localization of wheat germ agglutinin was markedly decreased in the basal layers and in regions of tumor invasion, accompanied by cytoplasmic redistribution of E-cadherin, -actin and syndecan-1. Wheat germ agglutinin staining clearly identified tumor clusters within the surrounding stroma, and tumor cells with elongated morphology. Conclusions: Our results suggest that the wheat germ agglutinin staining pattern is indicative of the degree of cell cohesion in oral squamous cell carcinoma, which decreases in basal layers and invasive tumor clusters with more migratory morphologies. Wheat germ agglutinin staining in combination with confocal microscopy could constitute, therefore, a valuable tool for the study of tissue architecture in oral cancer.Silveyra Estefanía, Universidad de la República (Uruguay) Facultad de OdontologíaBologna-Molina Ronell, Universidad de la República (Uruguay). Facultad de OdontologíaGónzalez-Gónzalez Rogelio, Department of Research, School of Dentistry, Universidad Juárez del Estado de DurangoArocena Miguel, Universidad de la República (Uruguay). Facultad de Odontología. Ministerio de Educación y Cultura. Instituto de Investigaciones Biológicas Clemente Estable2022-05-04T17:20:15Z2022-05-04T17:20:15Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfSilveyra, E, Bologna-Molina, R, Gónzalez-Gónzalez, R y otros. "The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy". Cells 2021. [en línea] 2021 10, 2466.https://doi.org/10.3390/cells10092466https://hdl.handle.net/20.500.12008/31424https://doi.org/10.3390/cells10092466reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengCells 2021, 10, 2466.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)oai:colibri.udelar.edu.uy:20.500.12008/314242026-04-14T10:37:12Z |
| spellingShingle | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy Silveyra, Estefanía Oral squamous cell carcinoma Wheat germ agglutinin Tyramide signal amplification Tissue architecture CARCINOMA DE CELULAS ESCAMOSAS AGLUTININAS DEL GERMEN DE TRIGO |
| status_str | publishedVersion |
| title | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| title_full | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| title_fullStr | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| title_full_unstemmed | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| title_short | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| title_sort | The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy |
| topic | Oral squamous cell carcinoma Wheat germ agglutinin Tyramide signal amplification Tissue architecture CARCINOMA DE CELULAS ESCAMOSAS AGLUTININAS DEL GERMEN DE TRIGO |
| url | https://hdl.handle.net/20.500.12008/31424 https://doi.org/10.3390/cells10092466 |