Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching

Carbonate radicals (CO3%-) are generated by the bicarbonate-dependent peroxidase activity of cytosolic superoxide dismutase (Cu,Zn-SOD, SOD-1). The present work explored the use of bleaching of pyrogallol red (PGR) dye to quantify the rate of CO3%- formation from bovine and human SOD-1 (bSOD-1 and h...

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Bibliographic Details

Main Author:	Figueroa, J.D. (author)
Other Authors:	Fuentes-Lemus, E. (author), Dorta, E. (author), Melin, V. (author), Cortés-Rios, J. (author), Faúndez, M. (author), Contretas, D. (author), Denicola, Ana (author), Álvarez, Beatriz (author), Davies, M.J. (author), López-Alarcón, C. (author)
Format:	article
Language:	English
Published:	2019
Subjects:	Carbonate radical anion Superoxide dismutase Hydrogen peroxide Pyrogallol red Peroxidase activity Human SOD-1 Bovine SOD-1 EPR DMPO ABTS
Online Access:	https://hdl.handle.net/20.500.12008/27231
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