Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation
Background: Multiple therapeutic properties have been attributed to Cannabis sativa. However, further research is required to unveil the medicinal potential of Cannabis and the relationship between biological activity and chemical profile. Objectives: The primary objective of this study was to chara...
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| Format: | article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/20.500.12008/31743 |
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| _version_ | 1868890105328959488 |
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| author | Musetti, Bruno |
| author2 | González-Ramos, Helena González, Mercedes Bahnson, Edward M. Varela Ubillos, Javier Alejandro Thomson, Leonor |
| author2_role | author author author author author |
| author_browse | Bahnson, Edward M. González, Mercedes González-Ramos, Helena Musetti, Bruno Thomson, Leonor Varela Ubillos, Javier Alejandro |
| author_facet | Musetti, Bruno González-Ramos, Helena González, Mercedes Bahnson, Edward M. Varela Ubillos, Javier Alejandro Thomson, Leonor |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Musetti Bruno, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. González-Ramos Helena, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. González Mercedes, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Bahnson Edward M., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Varela Ubillos Javier Alejandro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Thomson Leonor, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. |
| dc.creator.none.fl_str_mv | Musetti, Bruno González-Ramos, Helena González, Mercedes Bahnson, Edward M. Varela Ubillos, Javier Alejandro Thomson, Leonor |
| dc.date.none.fl_str_mv | 2020 2022-05-31T13:32:43Z 2022-05-31T13:32:43Z |
| dc.format.none.fl_str_mv | 11 h. application/pdf |
| dc.identifier.none.fl_str_mv | Musetti, B, González-Ramos, H, González, M, [y otros] "Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation". Journal of Cannabis Research. [en línea] 2020, 2: 37. 11 h. DOI: 10.1186/s42238-020-00042-0 2522-5782 https://hdl.handle.net/20.500.12008/31743 10.1186/s42238-020-00042-0 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | BMC |
| dc.relation.none.fl_str_mv | Journal of Cannabis Research, 2020, 2: 37 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Cannabis sativa Maturation Phytocannabinoids Hemp Low-density lipoprotein Oxidation Atherosclerosis |
| dc.title.none.fl_str_mv | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Background: Multiple therapeutic properties have been attributed to Cannabis sativa. However, further research is required to unveil the medicinal potential of Cannabis and the relationship between biological activity and chemical profile. Objectives: The primary objective of this study was to characterize the chemical profile and antioxidant properties of three varieties of Cannabis sativa available in Uruguay during progressive stages of maturation. Methods: Fresh samples of female inflorescences from three stable Cannabis sativa phenotypes, collected at different time points during the end of the flowering period were analyzed. Chemical characterization of chloroform extracts was performed by 1 H-NMR. The antioxidant properties of the Cannabis sativa extracts, and pure cannabinoids, were measured in a Cu2+-induced LDL oxidation assay. Results: The main cannabinoids in the youngest inflorescences were tetrahydrocannabinolic acid (THC-A, 242 ± 62 mg/g) and tetrahydrocannabinol (THC, 7.3 ± 6.5 mg/g). Cannabinoid levels increased more than twice in two of the mature samples. A third sample showed a lower and constant concentration of THC-A and THC (177 ± 25 and 1 ± 1, respectively). The THC-A/THC rich cannabis extracts increased the latency phase of LDL oxidation by a factor of 1.2–3.5 per μg, and slowed down the propagation phase of lipoperoxidation (IC50 1.7–4.6 μg/mL). Hemp, a cannabidiol (CBD, 198 mg/g) and cannabidiolic acid (CBD-A, 92 mg/g) rich variety, also prevented the formation of conjugated dienes during LDL oxidation. In fact, 1 μg of extract was able to stretch the latency phase 3.7 times and also to significantly reduce the steepness of the propagation phase (IC50 of 8 μg/mL). Synthetic THC lengthened the duration of the lag phase by a factor of 21 per μg, while for the propagation phase showed an IC50 ≤ 1 μg/mL. Conversely, THC-A was unable to improve any parameter. Meanwhile, the presence of 1 μg of pure CBD and CBD-A increased the initial latency phase 4.8 and 9.4 times, respectively, but did not have an effect on the propagation phase. Conclusion: Cannabis whole extracts acted on both phases of lipid oxidation in copper challenged LDL. Those effects were just partially related with the content of cannabinoids and partially recapitulated by isolated pure cannabinoids. Our results support the potentially beneficial effects of Cannabis sativa whole extracts on the initial phase of atherosclerosis. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_7b0fd255a8ce8006423dbda824d121f2 |
| identifier_str_mv | Musetti, B, González-Ramos, H, González, M, [y otros] "Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation". Journal of Cannabis Research. [en línea] 2020, 2: 37. 11 h. DOI: 10.1186/s42238-020-00042-0 2522-5782 10.1186/s42238-020-00042-0 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31743 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | BMC |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidationMusetti, BrunoGonzález-Ramos, HelenaGonzález, MercedesBahnson, Edward M.Varela Ubillos, Javier AlejandroThomson, LeonorCannabis sativaMaturationPhytocannabinoidsHempLow-density lipoproteinOxidationAtherosclerosisBackground: Multiple therapeutic properties have been attributed to Cannabis sativa. However, further research is required to unveil the medicinal potential of Cannabis and the relationship between biological activity and chemical profile. Objectives: The primary objective of this study was to characterize the chemical profile and antioxidant properties of three varieties of Cannabis sativa available in Uruguay during progressive stages of maturation. Methods: Fresh samples of female inflorescences from three stable Cannabis sativa phenotypes, collected at different time points during the end of the flowering period were analyzed. Chemical characterization of chloroform extracts was performed by 1 H-NMR. The antioxidant properties of the Cannabis sativa extracts, and pure cannabinoids, were measured in a Cu2+-induced LDL oxidation assay. Results: The main cannabinoids in the youngest inflorescences were tetrahydrocannabinolic acid (THC-A, 242 ± 62 mg/g) and tetrahydrocannabinol (THC, 7.3 ± 6.5 mg/g). Cannabinoid levels increased more than twice in two of the mature samples. A third sample showed a lower and constant concentration of THC-A and THC (177 ± 25 and 1 ± 1, respectively). The THC-A/THC rich cannabis extracts increased the latency phase of LDL oxidation by a factor of 1.2–3.5 per μg, and slowed down the propagation phase of lipoperoxidation (IC50 1.7–4.6 μg/mL). Hemp, a cannabidiol (CBD, 198 mg/g) and cannabidiolic acid (CBD-A, 92 mg/g) rich variety, also prevented the formation of conjugated dienes during LDL oxidation. In fact, 1 μg of extract was able to stretch the latency phase 3.7 times and also to significantly reduce the steepness of the propagation phase (IC50 of 8 μg/mL). Synthetic THC lengthened the duration of the lag phase by a factor of 21 per μg, while for the propagation phase showed an IC50 ≤ 1 μg/mL. Conversely, THC-A was unable to improve any parameter. Meanwhile, the presence of 1 μg of pure CBD and CBD-A increased the initial latency phase 4.8 and 9.4 times, respectively, but did not have an effect on the propagation phase. Conclusion: Cannabis whole extracts acted on both phases of lipid oxidation in copper challenged LDL. Those effects were just partially related with the content of cannabinoids and partially recapitulated by isolated pure cannabinoids. Our results support the potentially beneficial effects of Cannabis sativa whole extracts on the initial phase of atherosclerosis.BMCMusetti Bruno, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.González-Ramos Helena, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.González Mercedes, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Bahnson Edward M., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Varela Ubillos Javier Alejandro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Thomson Leonor, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.2022-05-31T13:32:43Z2022-05-31T13:32:43Z2020Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 h.application/pdfMusetti, B, González-Ramos, H, González, M, [y otros] "Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation". Journal of Cannabis Research. [en línea] 2020, 2: 37. 11 h. DOI: 10.1186/s42238-020-00042-02522-5782https://hdl.handle.net/20.500.12008/3174310.1186/s42238-020-00042-0reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Cannabis Research, 2020, 2: 37Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/317432026-04-14T10:09:47Z |
| spellingShingle | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation Musetti, Bruno Cannabis sativa Maturation Phytocannabinoids Hemp Low-density lipoprotein Oxidation Atherosclerosis |
| status_str | publishedVersion |
| title | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| title_full | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| title_fullStr | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| title_full_unstemmed | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| title_short | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| title_sort | Cannabis sativa extracts protect LDL from Cu2+-mediated oxidation |
| topic | Cannabis sativa Maturation Phytocannabinoids Hemp Low-density lipoprotein Oxidation Atherosclerosis |
| url | https://hdl.handle.net/20.500.12008/31743 |