Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes
Antimicrobial resistance is a critical issue that is no longer restricted to hospital settings but also represents a growing problem involving intensive animal production systems. In this study, we performed a microbiological and molecular investigation of priority pathogens carrying transferable re...
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| Format: | article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/20.500.12381/3977 https://doi.org/10.1128/AEM.01675-21 |
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| _version_ | 1868890096323788801 |
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| author | Coppola, Nadia |
| author2 | Cordeiro, Nicolás Trenchi, Gustavo Esposito, Fernanda Fuga, Bruna Fuentes-Castillo, Danny Lincopan, Nilton Iriarte, Andrés Bado, Inés Vignolia, Rafael |
| author2_role | author author author author author author author author author |
| author_browse | Bado, Inés Coppola, Nadia Cordeiro, Nicolás Esposito, Fernanda Fuentes-Castillo, Danny Fuga, Bruna Iriarte, Andrés Lincopan, Nilton Trenchi, Gustavo Vignolia, Rafael |
| author_facet | Coppola, Nadia Cordeiro, Nicolás Trenchi, Gustavo Esposito, Fernanda Fuga, Bruna Fuentes-Castillo, Danny Lincopan, Nilton Iriarte, Andrés Bado, Inés Vignolia, Rafael |
| author_role | author |
| collection | REDI |
| dc.creator.none.fl_str_mv | Coppola, Nadia Cordeiro, Nicolás Trenchi, Gustavo Esposito, Fernanda Fuga, Bruna Fuentes-Castillo, Danny Lincopan, Nilton Iriarte, Andrés Bado, Inés Vignolia, Rafael |
| dc.date.none.fl_str_mv | 2022-01-25 2025-05-12T12:24:51Z 2025-05-12T12:24:51Z |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/20.500.12381/3977 POS_FMV_2018_1_1007817 https://doi.org/10.1128/AEM.01675-21 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidad de los Andes |
| dc.relation.none.fl_str_mv | https://hdl.handle.net/20.500.12381/3973 |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Reconocimiento 4.0 Internacional. (CC BY) |
| dc.source.none.fl_str_mv | American Society for Microbiology. reponame:REDI instname:Agencia Nacional de Investigación e Innovación instacron:Agencia Nacional de Investigación e Innovación |
| dc.subject.none.fl_str_mv | Extended-spectrum b-lactamase Multidrug-resistant pathogens 1-dayold chicks mcr-9 rmtG Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología Ciencias Agrícolas Ciencias Veterinarias |
| dc.title.none.fl_str_mv | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | Antimicrobial resistance is a critical issue that is no longer restricted to hospital settings but also represents a growing problem involving intensive animal production systems. In this study, we performed a microbiological and molecular investigation of priority pathogens carrying transferable resistance genes to critical antimicrobials in 1-day-old chickens imported from Brazil to Uruguay. Bacterial identification was performed by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry, and antibiotic susceptibility was determined by Sensititre. Antimicrobial resistance genes were sought by PCR, and clonality was assessed by pulsed-field gel electrophoresis (PFGE). Four multidrug-resistant (MDR) representative strains were sequenced by an Illumina and/or Oxford Nanopore Technologies device. Twenty-eight MDR isolates were identified as Escherichia coli (n = 14), Enterobacter cloacae (n = 11), or Klebsiella pneumoniae (n = 3). While resistance to oxyiminocephalosporins was due to blaCTX-M-2, blaCTX-M-8, blaCTX-M-15, blaCTX-M-55, and blaCMY-2, plasmid-mediated quinolone resistance was associated with the qnrB19, qnrE1, and qnrB2 genes. Finally, resistance to aminoglycosides and fosfomycin was due to the presence of 16S rRNA methyltransferase rmtG and fosA-type genes, respectively. Short- and long-read genome sequencing of E. cloacae strain ODC_Eclo3 revealed the presence of IncQ/rmtG (pUR-EC3.1; 7,400 bp), IncHI2A/mcr- 9.1/blaCTX-M-2 (pUR-EC3.2, ST16 [pMLST; 408,436 bp), and IncN2/qnrB19/aacC3/aph(30)- Ib (pUR-EC3.3) resistance plasmids. Strikingly, the blaCTX-M-2 gene was carried by a novel Tn1696-like composite transposon designated Tn7337. In summary, we report that imported 1-day-old chicks can act as Trojan horses for the hidden spread of WHO critical-priority MDR pathogens harboring mcr-9, rmtG, and extended-spectrum b-lactamase genes in poultry farms, which is a critical issue from a One Health perspective. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_73f020a12ceaeb9d3d853fe782f351ff |
| identifier_str_mv | POS_FMV_2018_1_1007817 |
| instacron_str | Agencia Nacional de Investigación e Innovación |
| institution | Agencia Nacional de Investigación e Innovación |
| instname_str | Agencia Nacional de Investigación e Innovación |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/3977 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher.none.fl_str_mv | Universidad de los Andes |
| reponame_str | REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Reconocimiento 4.0 Internacional. (CC BY) |
| spelling | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase GenesCoppola, NadiaCordeiro, NicolásTrenchi, GustavoEsposito, FernandaFuga, BrunaFuentes-Castillo, DannyLincopan, NiltonIriarte, AndrésBado, InésVignolia, RafaelExtended-spectrum b-lactamaseMultidrug-resistant pathogens1-dayold chicksmcr-9rmtGCiencias Naturales y ExactasCiencias BiológicasBiología Celular, MicrobiologíaCiencias AgrícolasCiencias VeterinariasAntimicrobial resistance is a critical issue that is no longer restricted to hospital settings but also represents a growing problem involving intensive animal production systems. In this study, we performed a microbiological and molecular investigation of priority pathogens carrying transferable resistance genes to critical antimicrobials in 1-day-old chickens imported from Brazil to Uruguay. Bacterial identification was performed by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry, and antibiotic susceptibility was determined by Sensititre. Antimicrobial resistance genes were sought by PCR, and clonality was assessed by pulsed-field gel electrophoresis (PFGE). Four multidrug-resistant (MDR) representative strains were sequenced by an Illumina and/or Oxford Nanopore Technologies device. Twenty-eight MDR isolates were identified as Escherichia coli (n = 14), Enterobacter cloacae (n = 11), or Klebsiella pneumoniae (n = 3). While resistance to oxyiminocephalosporins was due to blaCTX-M-2, blaCTX-M-8, blaCTX-M-15, blaCTX-M-55, and blaCMY-2, plasmid-mediated quinolone resistance was associated with the qnrB19, qnrE1, and qnrB2 genes. Finally, resistance to aminoglycosides and fosfomycin was due to the presence of 16S rRNA methyltransferase rmtG and fosA-type genes, respectively. Short- and long-read genome sequencing of E. cloacae strain ODC_Eclo3 revealed the presence of IncQ/rmtG (pUR-EC3.1; 7,400 bp), IncHI2A/mcr- 9.1/blaCTX-M-2 (pUR-EC3.2, ST16 [pMLST; 408,436 bp), and IncN2/qnrB19/aacC3/aph(30)- Ib (pUR-EC3.3) resistance plasmids. Strikingly, the blaCTX-M-2 gene was carried by a novel Tn1696-like composite transposon designated Tn7337. In summary, we report that imported 1-day-old chicks can act as Trojan horses for the hidden spread of WHO critical-priority MDR pathogens harboring mcr-9, rmtG, and extended-spectrum b-lactamase genes in poultry farms, which is a critical issue from a One Health perspective.Agencia Nacional de Investigación e InnovaciónUniversidad de los Andes2025-05-12T12:24:51Z2025-05-12T12:24:51Z2022-01-25Artículoinfo:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/20.500.12381/3977POS_FMV_2018_1_1007817https://doi.org/10.1128/AEM.01675-21American Society for Microbiology.reponame:REDIinstname:Agencia Nacional de Investigación e Innovacióninstacron:Agencia Nacional de Investigación e Innovaciónenghttps://hdl.handle.net/20.500.12381/3973Acceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento 4.0 Internacional. (CC BY)oai:redi.anii.org.uy:20.500.12381/39772026-06-16T05:02:34Z |
| spellingShingle | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes Coppola, Nadia Extended-spectrum b-lactamase Multidrug-resistant pathogens 1-dayold chicks mcr-9 rmtG Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología Ciencias Agrícolas Ciencias Veterinarias |
| status_str | publishedVersion |
| title | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| title_full | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| title_fullStr | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| title_full_unstemmed | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| title_short | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| title_sort | Imported One-Day-Old Chicks as Trojan Horses for Multidrug- Resistant Priority Pathogens Harboring mcr-9, rmtG, and Extended-Spectrum b-Lactamase Genes |
| topic | Extended-spectrum b-lactamase Multidrug-resistant pathogens 1-dayold chicks mcr-9 rmtG Ciencias Naturales y Exactas Ciencias Biológicas Biología Celular, Microbiología Ciencias Agrícolas Ciencias Veterinarias |
| url | https://hdl.handle.net/20.500.12381/3977 https://doi.org/10.1128/AEM.01675-21 |