The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens
APETALA2/ethylene response factors (AP2/ERFs) transcription factors (TFs) have greatly expanded in land plants compared to algae. In angiosperms, AP2/ERFs play important regulatory functions in plant defenses against pathogens and abiotic stress by controlling the expression of target genes. In the...
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| Format: | article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/20.500.12008/41005 |
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| _version_ | 1868890096152870912 |
|---|---|
| author | Reboledo, Guillermo |
| author2 | Agorio, Astrid Vignale Alcarraz, Lucía Álvarez Tapié, Alfonso Ponce de León Tadeo, Inés |
| author2_role | author author author author |
| author_browse | Agorio, Astrid Ponce de León Tadeo, Inés Reboledo, Guillermo Vignale Alcarraz, Lucía Álvarez Tapié, Alfonso |
| author_facet | Reboledo, Guillermo Agorio, Astrid Vignale Alcarraz, Lucía Álvarez Tapié, Alfonso Ponce de León Tadeo, Inés |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Reboledo Guillermo, IIBCE Agorio Astrid, IIBCE Vignale Alcarraz Lucía, IIBCE Álvarez Tapié Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Ponce de León Tadeo Inés, IIBCE |
| dc.creator.none.fl_str_mv | Reboledo, Guillermo Agorio, Astrid Vignale Alcarraz, Lucía Álvarez Tapié, Alfonso Ponce de León Tadeo, Inés |
| dc.date.none.fl_str_mv | 2022 2023-11-08T15:33:57Z 2023-11-08T15:33:57Z |
| dc.format.none.fl_str_mv | 19 h. application/pdf |
| dc.identifier.none.fl_str_mv | Reboledo, G, Agorio, A, Vignale Alcarraz, L, [y otros autores]. "The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens". Frontiers in Plant Science. [en línea] 2022, 13: 908682. 19 h. DOI: 10.3389/fpls.2022.908682 1664-462X https://hdl.handle.net/20.500.12008/41005 10.3389/fpls.2022.908682 |
| dc.language.none.fl_str_mv | en_US eng |
| dc.publisher.none.fl_str_mv | Frontiers Media |
| dc.relation.none.fl_str_mv | Frontiers in Plant Science, 2022, 13: 908682. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Transcription factor AP2/ERF Physcomitrium patens Pathogens Defense |
| dc.title.none.fl_str_mv | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | APETALA2/ethylene response factors (AP2/ERFs) transcription factors (TFs) have greatly expanded in land plants compared to algae. In angiosperms, AP2/ERFs play important regulatory functions in plant defenses against pathogens and abiotic stress by controlling the expression of target genes. In the moss Physcomitrium patens, a high number of members of the ERF family are induced during pathogen infection, suggesting that they are important regulators in bryophyte immunity. In the current study, we analyzed a P. patens pathogen-inducible ERF family member designated as PpERF24. Orthologs of PpERF24 were only found in other mosses, while they were absent in the bryophytes Marchantia polymorpha and Anthoceros agrestis, the vascular plant Selaginella moellendorffii, and angiosperms. We show that PpERF24 belongs to a moss-specific clade with distinctive amino acids features in the AP2 domain that binds to the DNA. Interestingly, all P. patens members of the PpERF24 subclade are induced by fungal pathogens. The function of PpERF24 during plant immunity was assessed by an overexpression approach and transcriptomic analysis. Overexpressing lines showed increased defenses to infection by the fungal pathogens Botrytis cinerea and Colletotrichum gloeosporioides evidenced by reduced cellular damage and fungal biomass compared to wild-type plants. Transcriptomic and RT-qPCR analysis revealed that PpERF24 positively regulates the expression levels of defense genes involved in transcriptional regulation, phenylpropanoid and jasmonate pathways, oxidative burst and pathogenesis-related (PR) genes. These findings give novel insights into potential mechanism by which PpERF24 increases plant defenses against several pathogens by regulating important players in plant immunity. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_73a0dbf6e5a2f59504a9d72cc7dafde8 |
| identifier_str_mv | Reboledo, G, Agorio, A, Vignale Alcarraz, L, [y otros autores]. "The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens". Frontiers in Plant Science. [en línea] 2022, 13: 908682. 19 h. DOI: 10.3389/fpls.2022.908682 1664-462X 10.3389/fpls.2022.908682 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en_US |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/41005 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher.none.fl_str_mv | Frontiers Media |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patensReboledo, GuillermoAgorio, AstridVignale Alcarraz, LucíaÁlvarez Tapié, AlfonsoPonce de León Tadeo, InésTranscription factorAP2/ERFPhyscomitrium patensPathogensDefenseAPETALA2/ethylene response factors (AP2/ERFs) transcription factors (TFs) have greatly expanded in land plants compared to algae. In angiosperms, AP2/ERFs play important regulatory functions in plant defenses against pathogens and abiotic stress by controlling the expression of target genes. In the moss Physcomitrium patens, a high number of members of the ERF family are induced during pathogen infection, suggesting that they are important regulators in bryophyte immunity. In the current study, we analyzed a P. patens pathogen-inducible ERF family member designated as PpERF24. Orthologs of PpERF24 were only found in other mosses, while they were absent in the bryophytes Marchantia polymorpha and Anthoceros agrestis, the vascular plant Selaginella moellendorffii, and angiosperms. We show that PpERF24 belongs to a moss-specific clade with distinctive amino acids features in the AP2 domain that binds to the DNA. Interestingly, all P. patens members of the PpERF24 subclade are induced by fungal pathogens. The function of PpERF24 during plant immunity was assessed by an overexpression approach and transcriptomic analysis. Overexpressing lines showed increased defenses to infection by the fungal pathogens Botrytis cinerea and Colletotrichum gloeosporioides evidenced by reduced cellular damage and fungal biomass compared to wild-type plants. Transcriptomic and RT-qPCR analysis revealed that PpERF24 positively regulates the expression levels of defense genes involved in transcriptional regulation, phenylpropanoid and jasmonate pathways, oxidative burst and pathogenesis-related (PR) genes. These findings give novel insights into potential mechanism by which PpERF24 increases plant defenses against several pathogens by regulating important players in plant immunity.Frontiers MediaReboledo Guillermo, IIBCEAgorio Astrid, IIBCEVignale Alcarraz Lucía, IIBCEÁlvarez Tapié Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Ponce de León Tadeo Inés, IIBCE2023-11-08T15:33:57Z2023-11-08T15:33:57Z2022Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion19 h.application/pdfReboledo, G, Agorio, A, Vignale Alcarraz, L, [y otros autores]. "The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens". Frontiers in Plant Science. [en línea] 2022, 13: 908682. 19 h. DOI: 10.3389/fpls.2022.9086821664-462Xhttps://hdl.handle.net/20.500.12008/4100510.3389/fpls.2022.908682reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la Repúblicaen_USengFrontiers in Plant Science, 2022, 13: 908682.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/410052026-04-14T10:10:18Z |
| spellingShingle | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens Reboledo, Guillermo Transcription factor AP2/ERF Physcomitrium patens Pathogens Defense |
| status_str | publishedVersion |
| title | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| title_full | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| title_fullStr | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| title_full_unstemmed | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| title_short | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| title_sort | The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens in Physcomitrium patens |
| topic | Transcription factor AP2/ERF Physcomitrium patens Pathogens Defense |
| url | https://hdl.handle.net/20.500.12008/41005 |