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Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors

Xylitol, a five-carbon polyalcohol, is used in the food and pharmaceutical industries and as a building block in the synthesis of high-value chemicals. It can be sustainably produced from renewable sources through xylose assimilating microbe fermentation. Results: We screened microbial strains for x...

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Main Author: BONFIGLIO, FERNANDO (author)
Other Authors: CAGNO, MATÍAS (author), NÚÑEZ, LUCÍA (author), CASTRO, ROSSINA (author), BOTTO, EMILIANA (author), RODRÍGUEZ, PAULA (author)
Format: article
Language:English
Published: 2024
Subjects:
BIOMASA
CELULOSA
FERMENTACIÓN
Online Access:https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32876
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author BONFIGLIO, FERNANDO
author2 CAGNO, MATÍAS
NÚÑEZ, LUCÍA
CASTRO, ROSSINA
BOTTO, EMILIANA
RODRÍGUEZ, PAULA
author2_role author
author
author
author
author
author_browse BONFIGLIO, FERNANDO
BOTTO, EMILIANA
CAGNO, MATÍAS
CASTRO, ROSSINA
NÚÑEZ, LUCÍA
RODRÍGUEZ, PAULA
author_facet BONFIGLIO, FERNANDO
CAGNO, MATÍAS
NÚÑEZ, LUCÍA
CASTRO, ROSSINA
BOTTO, EMILIANA
RODRÍGUEZ, PAULA
author_role author
collection Catálogo digital del LATU
dc.coverage.none.fl_str_mv En: Electronic Journal of Biotechnology, 71, pp. 37–46. DOI: https://doi.org/10.1016/j.ejbt.2024.05.004
dc.creator.none.fl_str_mv BONFIGLIO, FERNANDO
CAGNO, MATÍAS
NÚÑEZ, LUCÍA
CASTRO, ROSSINA
BOTTO, EMILIANA
RODRÍGUEZ, PAULA
dc.date.none.fl_str_mv 2024-01-01
dc.format.none.fl_str_mv Pdf
dc.identifier.none.fl_str_mv https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32876
32876
urn:ISBN:69738
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv https://doi.org/10.1016/j.ejbt.2024.05.004
info:eu-repo/semantics/openAccess
CC BY
CC BY
dc.source.none.fl_str_mv reponame:Catálogo digital del LATU
instname:Laboratorio Tecnológico del Uruguay
instacron:Laboratorio Tecnológico del Uruguay
dc.subject.none.fl_str_mv BIOMASA
CELULOSA
FERMENTACIÓN
dc.title.none.fl_str_mv Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Publicado
info:eu-repo/semantics/publishedVersion
description Xylitol, a five-carbon polyalcohol, is used in the food and pharmaceutical industries and as a building block in the synthesis of high-value chemicals. It can be sustainably produced from renewable sources through xylose assimilating microbe fermentation. Results: We screened microbial strains for xylitol production and identified Wickerhamomyces anomalus Z1 as a key xylitol producer. Utilizing lignocellulosic biomass hydrolysates for xylitol production poses challenges due to microbial sensitivity to inhibitors from biomass pre-treatment. In this study, an adaptive laboratory evolution (ALE) of W. anomalus Z1 was performed by culturing the yeast in a mineral medium supplemented with gradual increases of sugarcane bagasse hemicellulosic hydrolysate (SCHH) obtained by intensified steam explosion pretreatment. The performance of the adapted yeast, named Wickerhamomyces anomalus ALE, was assessed in comparison to the wild-type strain regarding its capacity to produce xylitol using SCHH. The evolved yeast reached a xylitol yield of 0.11 g xylitol/g xylose whereas the wild-type strain could not produce xylitol. Removing acetic acid from SCHH enhanced W.
eu_rights_str_mv openAccess
format article
id anni_7253f4805d813b5b0b0cd3812437368f
identifier_str_mv 32876
urn:ISBN:69738
instacron_str Laboratorio Tecnológico del Uruguay
institution Laboratorio Tecnológico del Uruguay
instname_str Laboratorio Tecnológico del Uruguay
language eng
network_acronym_str anni
network_name_str oai-lr-anni
oai_identifier_str oai:PMBOAI:32876
publishDate 2024
publishDateSort 2024
reponame_str Catálogo digital del LATU
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv https://doi.org/10.1016/j.ejbt.2024.05.004
CC BY
CC BY
spelling Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitorsBONFIGLIO, FERNANDOCAGNO, MATÍASNÚÑEZ, LUCÍACASTRO, ROSSINABOTTO, EMILIANARODRÍGUEZ, PAULABIOMASACELULOSAFERMENTACIÓNXylitol, a five-carbon polyalcohol, is used in the food and pharmaceutical industries and as a building block in the synthesis of high-value chemicals. It can be sustainably produced from renewable sources through xylose assimilating microbe fermentation. Results: We screened microbial strains for xylitol production and identified Wickerhamomyces anomalus Z1 as a key xylitol producer. Utilizing lignocellulosic biomass hydrolysates for xylitol production poses challenges due to microbial sensitivity to inhibitors from biomass pre-treatment. In this study, an adaptive laboratory evolution (ALE) of W. anomalus Z1 was performed by culturing the yeast in a mineral medium supplemented with gradual increases of sugarcane bagasse hemicellulosic hydrolysate (SCHH) obtained by intensified steam explosion pretreatment. The performance of the adapted yeast, named Wickerhamomyces anomalus ALE, was assessed in comparison to the wild-type strain regarding its capacity to produce xylitol using SCHH. The evolved yeast reached a xylitol yield of 0.11 g xylitol/g xylose whereas the wild-type strain could not produce xylitol. Removing acetic acid from SCHH enhanced W.2024-01-01info:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionPdfhttps://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=3287632876urn:ISBN:69738reponame:Catálogo digital del LATUinstname:Laboratorio Tecnológico del Uruguayinstacron:Laboratorio Tecnológico del UruguayengEn: Electronic Journal of Biotechnology, 71, pp. 37–46. DOI: https://doi.org/10.1016/j.ejbt.2024.05.004https://doi.org/10.1016/j.ejbt.2024.05.004info:eu-repo/semantics/openAccessCC BYCC BYoai:PMBOAI:328762026-06-16T06:18:39Z
spellingShingle Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
BONFIGLIO, FERNANDO
BIOMASA
CELULOSA
FERMENTACIÓN
status_str publishedVersion
title Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
title_full Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
title_fullStr Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
title_full_unstemmed Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
title_short Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
title_sort Xylitol production by a Wickerhamomyces anomalus strain adapted for enhanced tolerance to sugarcane bagasse hemicellulosic hydrolysate with high content of fermentation inhibitors
topic BIOMASA
CELULOSA
FERMENTACIÓN
url https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32876

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