Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages
Background: The horn fly Haematobia irritans is a blood-sucking ectoparasite responsible for substantial economic loss of livestock. Like other hematophagous arthropods species, the successful blood-feeding of H. irritans is highly dependent on the modulation of the host’s hemostasis and immune syst...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12008/50616 https://doi.org/10.1186/s13071-018-3017-z |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1868890088137555968 |
|---|---|
| author | Breijo Dotta, Martín Andrés |
| author2 | Esteves, Eliane Bizzarro, Bruna Lara, Priscila G. Assis, Josiane B. Rocha, Sergio Pastro, Lucía Fernández, Cecilia Meikle, Ana Sá-Nunes, Anderson |
| author2_role | author author author author author author author author author |
| author_browse | Assis, Josiane B. Bizzarro, Bruna Breijo Dotta, Martín Andrés Esteves, Eliane Fernández, Cecilia Lara, Priscila G. Meikle, Ana Pastro, Lucía Rocha, Sergio Sá-Nunes, Anderson |
| author_facet | Breijo Dotta, Martín Andrés Esteves, Eliane Bizzarro, Bruna Lara, Priscila G. Assis, Josiane B. Rocha, Sergio Pastro, Lucía Fernández, Cecilia Meikle, Ana Sá-Nunes, Anderson |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Breijo Dotta Martín Andrés, Universidad de la República (Uruguay). Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación Esteves Eliane, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology Bizzarro Bruna, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology Lara Priscila G., University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology Assis Josiane B., University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology Rocha Sergio, Universidad de la República (Uruguay). Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación Pastro Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Laboratorio de Interacciones Moleculares Fernández Cecilia, Universidad de la República (Uruguay). Facultad de Química. Cátedra de Inmunología Meikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Técnicas Nucleares Sá-Nunes Anderson, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology ; National Council of Scientific and Technological Development (INCT-EM/CNPq) (Rio de Janeiro, RJ, Brazil.). National Institute of Science and Technology in Molecular Entomology |
| dc.creator.none.fl_str_mv | Breijo Dotta, Martín Andrés Esteves, Eliane Bizzarro, Bruna Lara, Priscila G. Assis, Josiane B. Rocha, Sergio Pastro, Lucía Fernández, Cecilia Meikle, Ana Sá-Nunes, Anderson |
| dc.date.none.fl_str_mv | 2018 2025-07-15T12:21:33Z 2025-07-15T12:21:33Z |
| dc.format.none.fl_str_mv | 11 p application/pdf |
| dc.identifier.none.fl_str_mv | Breijo Dotta, M, Esteves, E, Bizzarro, B, Lara, P, Assis, J, Rocha, S, Pastro, L, Fernández, C, Meikle, A y Sá-Nunes, A. Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages. Parasites & Vectors. [en línea] 2018, 11(435), 1-11 https://hdl.handle.net/20.500.12008/50616 https://doi.org/10.1186/s13071-018-3017-z |
| dc.language.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Parasites & Vectors, 2018, 11(435), 1-11 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | HAEMATOBIA IRRITANS SALIVA SANGRE ECTOPARASITOS RESPUESTA INMUNOLÓGICA |
| dc.title.none.fl_str_mv | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Background: The horn fly Haematobia irritans is a blood-sucking ectoparasite responsible for substantial economic loss of livestock. Like other hematophagous arthropods species, the successful blood-feeding of H. irritans is highly dependent on the modulation of the host’s hemostasis and immune system. Here, we evaluated the biological activity of hematobin (HTB), a protein recently identified in the H. irritans saliva, on macrophage biology. The goal was to understand the putative interactions between the components of H. irritans saliva and the early host immune responses. Results: Thioglycolate-elicited peritoneal macrophages from BALB/c mice were stimulated by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ) in the presence or absence of recombinant HTB. The presence of the salivary protein in the cultures inhibited nitric oxide production and decreased the inducible nitric oxide synthase (iNOS) expression induced by LPS plus IFN-γ. The tumor necrosis factor-α (TNF-α) and interleukin-12p40 (IL-12p40) levels were also reduced in the macrophages pre-incubated with HTB; these findings correlated to the decreased NF-κB expression. The biological activities described here were not associated with changes in annexin V binding to macrophages suggesting that HTB does not induce cell death. In addition, the activity of HTB seems to be specific to macrophages because no changes were observed in lymphocyte proliferation or cytokine production. Conclusions: We describe here the first bioactive salivary protein of H. irritans. We characterized its ability to modulate macrophage inflammatory response, and the results can help explain how horn flies modulate the host immune system to feed on blood. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_6c3a3d11d90ee00405b5be59ed3bbfaf |
| identifier_str_mv | Breijo Dotta, M, Esteves, E, Bizzarro, B, Lara, P, Assis, J, Rocha, S, Pastro, L, Fernández, C, Meikle, A y Sá-Nunes, A. Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages. Parasites & Vectors. [en línea] 2018, 11(435), 1-11 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50616 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophagesBreijo Dotta, Martín AndrésEsteves, ElianeBizzarro, BrunaLara, Priscila G.Assis, Josiane B.Rocha, SergioPastro, LucíaFernández, CeciliaMeikle, AnaSá-Nunes, AndersonHAEMATOBIA IRRITANSSALIVASANGREECTOPARASITOSRESPUESTA INMUNOLÓGICABackground: The horn fly Haematobia irritans is a blood-sucking ectoparasite responsible for substantial economic loss of livestock. Like other hematophagous arthropods species, the successful blood-feeding of H. irritans is highly dependent on the modulation of the host’s hemostasis and immune system. Here, we evaluated the biological activity of hematobin (HTB), a protein recently identified in the H. irritans saliva, on macrophage biology. The goal was to understand the putative interactions between the components of H. irritans saliva and the early host immune responses. Results: Thioglycolate-elicited peritoneal macrophages from BALB/c mice were stimulated by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ) in the presence or absence of recombinant HTB. The presence of the salivary protein in the cultures inhibited nitric oxide production and decreased the inducible nitric oxide synthase (iNOS) expression induced by LPS plus IFN-γ. The tumor necrosis factor-α (TNF-α) and interleukin-12p40 (IL-12p40) levels were also reduced in the macrophages pre-incubated with HTB; these findings correlated to the decreased NF-κB expression. The biological activities described here were not associated with changes in annexin V binding to macrophages suggesting that HTB does not induce cell death. In addition, the activity of HTB seems to be specific to macrophages because no changes were observed in lymphocyte proliferation or cytokine production. Conclusions: We describe here the first bioactive salivary protein of H. irritans. We characterized its ability to modulate macrophage inflammatory response, and the results can help explain how horn flies modulate the host immune system to feed on blood.Breijo Dotta Martín Andrés, Universidad de la República (Uruguay). Facultad de Medicina. Unidad de Reactivos y Biomodelos de ExperimentaciónEsteves Eliane, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental ImmunologyBizzarro Bruna, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental ImmunologyLara Priscila G., University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental ImmunologyAssis Josiane B., University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental ImmunologyRocha Sergio, Universidad de la República (Uruguay). Facultad de Medicina. Unidad de Reactivos y Biomodelos de ExperimentaciónPastro Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Laboratorio de Interacciones MolecularesFernández Cecilia, Universidad de la República (Uruguay). Facultad de Química. Cátedra de InmunologíaMeikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Laboratorio de Técnicas NuclearesSá-Nunes Anderson, University of Sao Paulo (Sao Paulo, Brazil) Department of Immunology. Institute of Biomedical Sciences. Laboratory of Experimental Immunology ; National Council of Scientific and Technological Development (INCT-EM/CNPq) (Rio de Janeiro, RJ, Brazil.). National Institute of Science and Technology in Molecular Entomology2025-07-15T12:21:33Z2025-07-15T12:21:33Z2018Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 papplication/pdfBreijo Dotta, M, Esteves, E, Bizzarro, B, Lara, P, Assis, J, Rocha, S, Pastro, L, Fernández, C, Meikle, A y Sá-Nunes, A. Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages. Parasites & Vectors. [en línea] 2018, 11(435), 1-11https://hdl.handle.net/20.500.12008/50616https://doi.org/10.1186/s13071-018-3017-zreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengParasites & Vectors, 2018, 11(435), 1-11Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/506162026-04-14T10:35:06Z |
| spellingShingle | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages Breijo Dotta, Martín Andrés HAEMATOBIA IRRITANS SALIVA SANGRE ECTOPARASITOS RESPUESTA INMUNOLÓGICA |
| status_str | publishedVersion |
| title | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| title_full | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| title_fullStr | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| title_full_unstemmed | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| title_short | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| title_sort | Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages |
| topic | HAEMATOBIA IRRITANS SALIVA SANGRE ECTOPARASITOS RESPUESTA INMUNOLÓGICA |
| url | https://hdl.handle.net/20.500.12008/50616 https://doi.org/10.1186/s13071-018-3017-z |