Biomolecular condensates modulate membrane lipid packing and hydration
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membra...
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| Other Authors: | , , , , |
| Format: | article |
| Language: | English |
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2023
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| Online Access: | https://hdl.handle.net/20.500.12008/53295 |
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| _version_ | 1868890066959466496 |
|---|---|
| author | Mangiarotti, Agustín |
| author2 | Siri, Macarena Tam, Nicky W. Zhao, Ziliang Malacrida, Leonel Dimova, Rumiana |
| author2_role | author author author author author |
| author_browse | Dimova, Rumiana Malacrida, Leonel Mangiarotti, Agustín Siri, Macarena Tam, Nicky W. Zhao, Ziliang |
| author_facet | Mangiarotti, Agustín Siri, Macarena Tam, Nicky W. Zhao, Ziliang Malacrida, Leonel Dimova, Rumiana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Mangiarotti Agustín, Max Planck Institute of Colloids and Interfaces (Alemania) Siri Macarena, Max Planck Institute of Colloids and Interfaces (Alemania) Tam Nicky W., Max Planck Institute of Colloids and Interfaces (Alemania) Zhao Ziliang, Leibniz Institute of Photonic Technology (Alemania) Malacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina. Departamento de Fisiopatología Dimova Rumiana, Max Planck Institute of Colloids and Interfaces (Alemania) |
| dc.creator.none.fl_str_mv | Mangiarotti, Agustín Siri, Macarena Tam, Nicky W. Zhao, Ziliang Malacrida, Leonel Dimova, Rumiana |
| dc.date.none.fl_str_mv | 2023 2026-01-28T14:03:03Z 2026-01-28T14:03:03Z |
| dc.format.none.fl_str_mv | 19 p. application/pdf |
| dc.identifier.none.fl_str_mv | Mangiarotti A, Siri M, Tam N y otros. Biomolecular condensates modulate membrane lipid packing and hydration. Nature Communications [en línea]. 2023;14(1). 19 p. 2041-1723 https://hdl.handle.net/20.500.12008/53295 10.1038/s41467-023-41709-5 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Nature Research |
| dc.relation.none.fl_str_mv | Nature Communications. 2023;14(1) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | CONDENSADOS BIOMOLECULARES LÍPIDOS DE LA MEMBRANA BIOLOGÍA CELULAR MICROSCOPÍA IMÁGENES HIPERESPECTRALES AMINOÁCIDOS ESTRUCTURA SECUNDARIA DE PROTEÍNA |
| dc.title.none.fl_str_mv | Biomolecular condensates modulate membrane lipid packing and hydration |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited the properties of the dyes ACDAN and LAURDAN as nano-environmental sensors in combination with phasor analysis of hyperspectral and lifetime imaging microscopy. Using glycinin as a model condensate-forming protein and giant vesicles as model membranes, we obtained vital information on the process of condensate formation and membrane wetting. Our results reveal that glycinin condensates display differences in water dynamics when changing the salinity of themedium as a consequence of rearrangements in the secondary structure of the protein. Remarkably, analysis of membrane-condensates interaction with protein as well as polymer condensates indicated a correlation between increasedwetting affinity and enhanced lipid packing. This is demonstrated by a decrease in the dipolar relaxation of water across all membrane-condensate systems, suggesting a general mechanism to tune membrane packing by condensate wetting. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_590be73ccfc37554d29b7db00aee2db1 |
| identifier_str_mv | Mangiarotti A, Siri M, Tam N y otros. Biomolecular condensates modulate membrane lipid packing and hydration. Nature Communications [en línea]. 2023;14(1). 19 p. 2041-1723 10.1038/s41467-023-41709-5 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/53295 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | Nature Research |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Biomolecular condensates modulate membrane lipid packing and hydrationMangiarotti, AgustínSiri, MacarenaTam, Nicky W.Zhao, ZiliangMalacrida, LeonelDimova, RumianaCONDENSADOS BIOMOLECULARESLÍPIDOS DE LA MEMBRANABIOLOGÍA CELULARMICROSCOPÍAIMÁGENES HIPERESPECTRALESAMINOÁCIDOSESTRUCTURA SECUNDARIA DE PROTEÍNAMembrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited the properties of the dyes ACDAN and LAURDAN as nano-environmental sensors in combination with phasor analysis of hyperspectral and lifetime imaging microscopy. Using glycinin as a model condensate-forming protein and giant vesicles as model membranes, we obtained vital information on the process of condensate formation and membrane wetting. Our results reveal that glycinin condensates display differences in water dynamics when changing the salinity of themedium as a consequence of rearrangements in the secondary structure of the protein. Remarkably, analysis of membrane-condensates interaction with protein as well as polymer condensates indicated a correlation between increasedwetting affinity and enhanced lipid packing. This is demonstrated by a decrease in the dipolar relaxation of water across all membrane-condensate systems, suggesting a general mechanism to tune membrane packing by condensate wetting.Nature ResearchMangiarotti Agustín, Max Planck Institute of Colloids and Interfaces (Alemania)Siri Macarena, Max Planck Institute of Colloids and Interfaces (Alemania)Tam Nicky W., Max Planck Institute of Colloids and Interfaces (Alemania)Zhao Ziliang, Leibniz Institute of Photonic Technology (Alemania)Malacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina. Departamento de FisiopatologíaDimova Rumiana, Max Planck Institute of Colloids and Interfaces (Alemania)2026-01-28T14:03:03Z2026-01-28T14:03:03Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion19 p.application/pdfMangiarotti A, Siri M, Tam N y otros. Biomolecular condensates modulate membrane lipid packing and hydration. Nature Communications [en línea]. 2023;14(1). 19 p.2041-1723https://hdl.handle.net/20.500.12008/5329510.1038/s41467-023-41709-5reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengNature Communications. 2023;14(1)Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/532952026-04-14T10:28:25Z |
| spellingShingle | Biomolecular condensates modulate membrane lipid packing and hydration Mangiarotti, Agustín CONDENSADOS BIOMOLECULARES LÍPIDOS DE LA MEMBRANA BIOLOGÍA CELULAR MICROSCOPÍA IMÁGENES HIPERESPECTRALES AMINOÁCIDOS ESTRUCTURA SECUNDARIA DE PROTEÍNA |
| status_str | publishedVersion |
| title | Biomolecular condensates modulate membrane lipid packing and hydration |
| title_full | Biomolecular condensates modulate membrane lipid packing and hydration |
| title_fullStr | Biomolecular condensates modulate membrane lipid packing and hydration |
| title_full_unstemmed | Biomolecular condensates modulate membrane lipid packing and hydration |
| title_short | Biomolecular condensates modulate membrane lipid packing and hydration |
| title_sort | Biomolecular condensates modulate membrane lipid packing and hydration |
| topic | CONDENSADOS BIOMOLECULARES LÍPIDOS DE LA MEMBRANA BIOLOGÍA CELULAR MICROSCOPÍA IMÁGENES HIPERESPECTRALES AMINOÁCIDOS ESTRUCTURA SECUNDARIA DE PROTEÍNA |
| url | https://hdl.handle.net/20.500.12008/53295 |