Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
Cells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer areas...
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| اللغة: | الإنجليزية |
| منشور في: |
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://hdl.handle.net/20.500.12008/52077 |
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| _version_ | 1868890058148282368 |
|---|---|
| author | Aguilar, Joao |
| author2 | Malacrida, Leonel Gunther, Germán Torrado, Belén Urbano, Bruno F. Torres, Viviana Sánchez, Susana A. |
| author2_role | author author author author author author |
| author_browse | Aguilar, Joao Gunther, Germán Malacrida, Leonel Sánchez, Susana A. Torrado, Belén Torres, Viviana Urbano, Bruno F. |
| author_facet | Aguilar, Joao Malacrida, Leonel Gunther, Germán Torrado, Belén Urbano, Bruno F. Torres, Viviana Sánchez, Susana A. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Aguilar Joao, Universidad de Concepción (Chile). Facultad de Ciencias Químicas Malacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina Gunther Germán, Universidad de Chile (Chile). Departamento de Química Orgánica y Fisicoquímica Torrado Belén, University of California at Irvine (E.E.U.U.). Biomedical Engineering Department Urbano Bruno F., Universidad de Concepción (Chile). Facultad de Ciencias Químicas Torres Viviana, Universidad de Concepción (Chile). Facultad de Ciencias Biológicas Sánchez Susana A., Universidad de Concepción (Chile). Facultad de Ciencias Químicas |
| dc.creator.none.fl_str_mv | Aguilar, Joao Malacrida, Leonel Gunther, Germán Torrado, Belén Urbano, Bruno F. Torres, Viviana Sánchez, Susana A. |
| dc.date.none.fl_str_mv | 2023 2025-10-15T12:52:17Z 2025-10-15T12:52:17Z |
| dc.format.none.fl_str_mv | 10 p. application/pdf |
| dc.identifier.none.fl_str_mv | Aguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p. 1879-2642 https://hdl.handle.net/20.500.12008/52077 10.1016/j.bbamem.2023.184176 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.relation.none.fl_str_mv | Biochimica et Biophysica Acta (BBA) - Biomembranes. 2023;1865(7) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Membrane heterogeneity Spectral phasor analysis LAURDAN Membrane fluidity Collagen matrices Extracellular stiffness ESTRUCTURAS DE LA MEMBRANA CELULAR METABOLISMO COLÁGENO LAURATOS QUÍMICA FLUIDEZ DE LA MEMBRANA |
| dc.title.none.fl_str_mv | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Cells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer areas with different fluidities at the plasma membrane change their distribution in response to external mechanical signals. However, investigations linking fluidity domains with mechanical stimuli, specifically matrix stiffness, are still in progress. This report tests the hypothesis that the stiffness of the extracellular matrix can modify the equilibrium of areas with different order in the plasma membrane, resulting in changes in overall membrane fluidity distribution. We studied the effect of matrix stiffness on the distribution of membrane lipid domains in NIH-3 T3 cells immersed in matrices of varying concentrations of collagen type I, for 24 or 72 h. The stiffness and viscoelastic properties of the collagen matrices were characterized by rheometry, fiber sizes were measured by Scanning Electron Microscopy (SEM) and the volume occupied by the fibers by second harmonic generation imaging (SHG). Membrane fluidity was measured using the fluorescent dye LAURDAN and spectral phasor analysis. The results demonstrate that an increase in collagen stiffness alters the distribution of membrane fluidity, leading to an increasing amount of the LAURDAN fraction with a high degree of packing. These findings suggest that changes in the equilibrium of fluidity domains could represent a versatile and refined component of the signal transduction mechanism for cells to respond to the highly heterogeneous matrix structural composition. Overall, this study sheds light on the importance of the plasma membrane's role in adapting to the extracellular matrix's mechanical cues. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_542bb6936c8b903fbe21cd97a87b0dc8 |
| identifier_str_mv | Aguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p. 1879-2642 10.1016/j.bbamem.2023.184176 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/52077 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increasesAguilar, JoaoMalacrida, LeonelGunther, GermánTorrado, BelénUrbano, Bruno F.Torres, VivianaSánchez, Susana A.Membrane heterogeneitySpectral phasor analysisLAURDANMembrane fluidityCollagen matricesExtracellular stiffnessESTRUCTURAS DE LA MEMBRANA CELULARMETABOLISMOCOLÁGENOLAURATOSQUÍMICAFLUIDEZ DE LA MEMBRANACells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer areas with different fluidities at the plasma membrane change their distribution in response to external mechanical signals. However, investigations linking fluidity domains with mechanical stimuli, specifically matrix stiffness, are still in progress. This report tests the hypothesis that the stiffness of the extracellular matrix can modify the equilibrium of areas with different order in the plasma membrane, resulting in changes in overall membrane fluidity distribution. We studied the effect of matrix stiffness on the distribution of membrane lipid domains in NIH-3 T3 cells immersed in matrices of varying concentrations of collagen type I, for 24 or 72 h. The stiffness and viscoelastic properties of the collagen matrices were characterized by rheometry, fiber sizes were measured by Scanning Electron Microscopy (SEM) and the volume occupied by the fibers by second harmonic generation imaging (SHG). Membrane fluidity was measured using the fluorescent dye LAURDAN and spectral phasor analysis. The results demonstrate that an increase in collagen stiffness alters the distribution of membrane fluidity, leading to an increasing amount of the LAURDAN fraction with a high degree of packing. These findings suggest that changes in the equilibrium of fluidity domains could represent a versatile and refined component of the signal transduction mechanism for cells to respond to the highly heterogeneous matrix structural composition. Overall, this study sheds light on the importance of the plasma membrane's role in adapting to the extracellular matrix's mechanical cues.ElsevierAguilar Joao, Universidad de Concepción (Chile). Facultad de Ciencias QuímicasMalacrida Leonel, Universidad de la República (Uruguay). Facultad de MedicinaGunther Germán, Universidad de Chile (Chile). Departamento de Química Orgánica y FisicoquímicaTorrado Belén, University of California at Irvine (E.E.U.U.). Biomedical Engineering DepartmentUrbano Bruno F., Universidad de Concepción (Chile). Facultad de Ciencias QuímicasTorres Viviana, Universidad de Concepción (Chile). Facultad de Ciencias BiológicasSánchez Susana A., Universidad de Concepción (Chile). Facultad de Ciencias Químicas2025-10-15T12:52:17Z2025-10-15T12:52:17Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfAguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p.1879-2642https://hdl.handle.net/20.500.12008/5207710.1016/j.bbamem.2023.184176reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengBiochimica et Biophysica Acta (BBA) - Biomembranes. 2023;1865(7)Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/520772026-04-14T10:28:19Z |
| spellingShingle | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases Aguilar, Joao Membrane heterogeneity Spectral phasor analysis LAURDAN Membrane fluidity Collagen matrices Extracellular stiffness ESTRUCTURAS DE LA MEMBRANA CELULAR METABOLISMO COLÁGENO LAURATOS QUÍMICA FLUIDEZ DE LA MEMBRANA |
| status_str | publishedVersion |
| title | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| title_full | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| title_fullStr | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| title_full_unstemmed | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| title_short | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| title_sort | Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases |
| topic | Membrane heterogeneity Spectral phasor analysis LAURDAN Membrane fluidity Collagen matrices Extracellular stiffness ESTRUCTURAS DE LA MEMBRANA CELULAR METABOLISMO COLÁGENO LAURATOS QUÍMICA FLUIDEZ DE LA MEMBRANA |
| url | https://hdl.handle.net/20.500.12008/52077 |