piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids

piRNAs function as genome defense mechanisms against transposable elements insertions within germ line cells. Recent studies have unraveled that piRNA pathways are not limited to germ cells as initially reckoned, but are instead also found in non-gonadal somatic contexts. Moreover, these pathways ha...

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Hlavní autor: Horjales, Sofía (author)
Další autoři: Li Calzi, Marco (author), Francia, María E. (author), Cayota, Alfonso (author), Garcia-Silva, María Rosa (author)
Médium: article
Jazyk:angličtina
Vydáno: 2023
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On-line přístup:https://hdl.handle.net/20.500.12008/52746
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author Horjales, Sofía
author2 Li Calzi, Marco
Francia, María E.
Cayota, Alfonso
Garcia-Silva, María Rosa
author2_role author
author
author
author
author_browse Cayota, Alfonso
Francia, María E.
Garcia-Silva, María Rosa
Horjales, Sofía
Li Calzi, Marco
author_facet Horjales, Sofía
Li Calzi, Marco
Francia, María E.
Cayota, Alfonso
Garcia-Silva, María Rosa
author_role author
collection COLIBRI
dc.contributor.none.fl_str_mv Horjales Sofía, Institute Pasteur Montevideo (Uruguay)
Li Calzi Marco, Institute Pasteur Montevideo (Uruguay)
Francia María E., Universidad de la República (Uruguay). Facultad de Medicina
Cayota Alfonso, Universidad de la República (Uruguay). Facultad de Medicina
Garcia-Silva María Rosa, Institute Pasteur Montevideo (Uruguay)
dc.creator.none.fl_str_mv Horjales, Sofía
Li Calzi, Marco
Francia, María E.
Cayota, Alfonso
Garcia-Silva, María Rosa
dc.date.none.fl_str_mv 2023
2025-12-01T21:19:43Z
2025-12-01T21:19:43Z
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.identifier.none.fl_str_mv Horjales S, Li Calzi M, Francia M y otros. piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids[en línea]. Frontiers in Genetics. 2023;14 [en línea]. 7 p.
1664-8021
https://hdl.handle.net/20.500.12008/52746
10.3389/fgene.2023.1129194
dc.language.none.fl_str_mv en
eng
dc.publisher.none.fl_str_mv Frontiers
dc.relation.none.fl_str_mv Frontiers in Genetics. 2023;14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Licencia Creative Commons Atribución (CC - By 4.0)
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
dc.subject.none.fl_str_mv Argonautes (AGO)
PIWI
Toxoplasma
Trypanosoma
piRNA biogenesis
ARN DE INTERACCIÓN CON PIWI
TRYPANOSOMA
TOXOPLASMA
TOXOPLASMOSIS
APICOMPLEXA
PROTEÍNAS ARGONAUTAS
dc.title.none.fl_str_mv piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
dc.type.none.fl_str_mv Artículo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description piRNAs function as genome defense mechanisms against transposable elements insertions within germ line cells. Recent studies have unraveled that piRNA pathways are not limited to germ cells as initially reckoned, but are instead also found in non-gonadal somatic contexts. Moreover, these pathways have also been reported in bacteria, mollusks and arthropods, associated with safeguard of genomes against transposable elements, regulation of gene expression and with direct consequences in axon regeneration and memory formation. In this Perspective we draw attention to early branching parasitic protozoa, whose genome preservation is an essential function as in late eukaryotes. However, little is known about the defense mechanisms of these genomes. We and others have described the presence of putative PIWI-related machinery members in protozoan parasites. We have described the presence of a PIWI-like protein in Trypanosoma cruzi, bound to small non-coding RNAs (sRNAs) as cargo of secreted extracellular vesicles relevant in intercellular communication and host infection. Herein, we put forward the presence of members related to Argonaute pathways in both Trypanosoma cruzi and Toxoplasma gondii. The presence of PIWI-like machinery in Trypansomatids and Apicomplexa, respectively, could be evidence of an ancestral piRNA machinery that evolved to become more sophisticated and complex in multicellular eukaryotes. We propose a model in which ancient PIWI proteins were expressed broadly and had functions independent of germline maintenance. A better understanding of current and ancestral PIWI/piRNAs will be relevant to better understand key mechanisms of genome integrity conservation during cell cycle progression and modulation of host defense mechanisms by protozoan parasites.
eu_rights_str_mv openAccess
format article
id anni_5104f8d650d873c61cf23ac2120e33b7
identifier_str_mv Horjales S, Li Calzi M, Francia M y otros. piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids[en línea]. Frontiers in Genetics. 2023;14 [en línea]. 7 p.
1664-8021
10.3389/fgene.2023.1129194
instacron_str Universidad de la República
institution Universidad de la República
instname_str Universidad de la República
language eng
language_invalid_str_mv en
network_acronym_str anni
network_name_str oai-lr-anni
oai_identifier_str oai:colibri.udelar.edu.uy:20.500.12008/52746
publishDate 2023
publishDateSort 2023
publisher.none.fl_str_mv Frontiers
reponame_str COLIBRI
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
spelling piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatidsHorjales, SofíaLi Calzi, MarcoFrancia, María E.Cayota, AlfonsoGarcia-Silva, María RosaArgonautes (AGO)PIWIToxoplasmaTrypanosomapiRNA biogenesisARN DE INTERACCIÓN CON PIWITRYPANOSOMATOXOPLASMATOXOPLASMOSISAPICOMPLEXAPROTEÍNAS ARGONAUTASpiRNAs function as genome defense mechanisms against transposable elements insertions within germ line cells. Recent studies have unraveled that piRNA pathways are not limited to germ cells as initially reckoned, but are instead also found in non-gonadal somatic contexts. Moreover, these pathways have also been reported in bacteria, mollusks and arthropods, associated with safeguard of genomes against transposable elements, regulation of gene expression and with direct consequences in axon regeneration and memory formation. In this Perspective we draw attention to early branching parasitic protozoa, whose genome preservation is an essential function as in late eukaryotes. However, little is known about the defense mechanisms of these genomes. We and others have described the presence of putative PIWI-related machinery members in protozoan parasites. We have described the presence of a PIWI-like protein in Trypanosoma cruzi, bound to small non-coding RNAs (sRNAs) as cargo of secreted extracellular vesicles relevant in intercellular communication and host infection. Herein, we put forward the presence of members related to Argonaute pathways in both Trypanosoma cruzi and Toxoplasma gondii. The presence of PIWI-like machinery in Trypansomatids and Apicomplexa, respectively, could be evidence of an ancestral piRNA machinery that evolved to become more sophisticated and complex in multicellular eukaryotes. We propose a model in which ancient PIWI proteins were expressed broadly and had functions independent of germline maintenance. A better understanding of current and ancestral PIWI/piRNAs will be relevant to better understand key mechanisms of genome integrity conservation during cell cycle progression and modulation of host defense mechanisms by protozoan parasites.FrontiersHorjales Sofía, Institute Pasteur Montevideo (Uruguay)Li Calzi Marco, Institute Pasteur Montevideo (Uruguay)Francia María E., Universidad de la República (Uruguay). Facultad de MedicinaCayota Alfonso, Universidad de la República (Uruguay). Facultad de MedicinaGarcia-Silva María Rosa, Institute Pasteur Montevideo (Uruguay)2025-12-01T21:19:43Z2025-12-01T21:19:43Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfHorjales S, Li Calzi M, Francia M y otros. piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids[en línea]. Frontiers in Genetics. 2023;14 [en línea]. 7 p.1664-8021https://hdl.handle.net/20.500.12008/5274610.3389/fgene.2023.1129194reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengFrontiers in Genetics. 2023;14Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/527462026-04-14T10:28:23Z
spellingShingle piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
Horjales, Sofía
Argonautes (AGO)
PIWI
Toxoplasma
Trypanosoma
piRNA biogenesis
ARN DE INTERACCIÓN CON PIWI
TRYPANOSOMA
TOXOPLASMA
TOXOPLASMOSIS
APICOMPLEXA
PROTEÍNAS ARGONAUTAS
status_str publishedVersion
title piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
title_full piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
title_fullStr piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
title_full_unstemmed piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
title_short piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
title_sort piRNA pathway evolution beyond gonad context: Perspectives from apicomplexa and trypanosomatids
topic Argonautes (AGO)
PIWI
Toxoplasma
Trypanosoma
piRNA biogenesis
ARN DE INTERACCIÓN CON PIWI
TRYPANOSOMA
TOXOPLASMA
TOXOPLASMOSIS
APICOMPLEXA
PROTEÍNAS ARGONAUTAS
url https://hdl.handle.net/20.500.12008/52746