Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach
Delftia sp. JD2 is a chromium-resistant bacterium that reduces Cr(VI) to Cr(III), accumulates Pb(II), produces the phytohormone indole-3-acetic acid and siderophores, and increases the plant growth performance of rhizobia in co-inoculation experiments. We aimed to analyze the biotechnological potent...
সংরক্ষণ করুন:
| প্রধান লেখক: | |
|---|---|
| অন্যান্য লেখক: | , , , |
| বিন্যাস: | article |
| ভাষা: | ইংরেজি |
| প্রকাশিত: |
2016
|
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | http://hdl.handle.net/20.500.12381/168 https://doi.org/10.3934/bioeng.2016.2.156 |
| ট্যাগগুলো: |
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
|
| _version_ | 1868890044304982016 |
|---|---|
| author | Morel, María A. |
| author2 | Iriarte, Aandrés Jara, Eugenio Musto, Héctor Castro-Sowinski, Susana |
| author2_role | author author author author |
| author_browse | Castro-Sowinski, Susana Iriarte, Aandrés Jara, Eugenio Morel, María A. Musto, Héctor |
| author_facet | Morel, María A. Iriarte, Aandrés Jara, Eugenio Musto, Héctor Castro-Sowinski, Susana |
| author_role | author |
| collection | IIBCE en REDI |
| dc.creator.none.fl_str_mv | Morel, María A. Iriarte, Aandrés Jara, Eugenio Musto, Héctor Castro-Sowinski, Susana |
| dc.date.none.fl_str_mv | 2016 2019-10-25T14:35:37Z 2019-10-25T14:35:37Z |
| dc.format.none.fl_str_mv | 21 p. |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/20.500.12381/168 FMV_3_2011_1_6089 https://doi.org/10.3934/bioeng.2016.2.156 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | AIMS |
| dc.rights.none.fl_str_mv | Acceso abierto info:eu-repo/semantics/openAccess Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND) |
| dc.source.none.fl_str_mv | AIMS Bioengineering, 3(2): 156-175 reponame:IIBCE en REDI instname:Instituto de Investigaciones Biológicas Clemente Estable instacron:Instituto de Investigaciones Biológicas Clemente Estable |
| dc.subject.none.fl_str_mv | Delftia Comparative genomic Plant-microbe interaction Plant-growth promotion Bioremediation Ciencias Naturales y Exactas Ciencias de la Computación e Información Ciencias de la Información y Bioinformática Ciencias Biológicas |
| dc.title.none.fl_str_mv | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | Delftia sp. JD2 is a chromium-resistant bacterium that reduces Cr(VI) to Cr(III), accumulates Pb(II), produces the phytohormone indole-3-acetic acid and siderophores, and increases the plant growth performance of rhizobia in co-inoculation experiments. We aimed to analyze the biotechnological potential of JD2 using a genomic approach. JD2 has a genome of 6.76Mb, with 6,051 predicted protein-coding sequences and 93 RNA genes (tRNA and rRNA). The indole-acetamide pathway was identified as responsible for the synthesis of indole-3-acetic acid. The genetic information involved in chromium resistance (the gene cluster, chrBACF,) was found. At least 40 putative genes encoding for TonB-dependent receptors, probably involved in the utilization of siderophores and biopolymers, and genes for the synthesis, maturation, exportation and uptake of pyoverdine, and acquisition of Fe-pyochelin and Fe-enterobactin were also identified. The information also suggests that JD2 produce polyhydroxy butyrate, a carbon reserve polymer commonly used for manufacturing petrochemical free bioplastics. In addition, JD2 may degrade lignin-derived aromatic compounds to 2-pyrone-4,6-dicarboxylate, a molecule used in the bio-based polymer industry. Finally, a comparative genomic analysis of JD2, Delftia sp. Cs1-4 and Delftia acidovorans SPH-1 is also discussed. The present work provides insights into the physiology and genetics of a microorganism with many potential uses in biotechnology. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_4e6a3eb880c0d62a45ed69a6106e70ea |
| identifier_str_mv | FMV_3_2011_1_6089 |
| instacron_str | Instituto de Investigaciones Biológicas Clemente Estable |
| institution | Instituto de Investigaciones Biológicas Clemente Estable |
| instname_str | Instituto de Investigaciones Biológicas Clemente Estable |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/168 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher.none.fl_str_mv | AIMS |
| reponame_str | IIBCE en REDI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND) |
| spelling | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approachMorel, María A.Iriarte, AandrésJara, EugenioMusto, HéctorCastro-Sowinski, SusanaDelftiaComparative genomicPlant-microbe interactionPlant-growth promotionBioremediationCiencias Naturales y ExactasCiencias de la Computación e InformaciónCiencias de la Información y BioinformáticaCiencias BiológicasDelftia sp. JD2 is a chromium-resistant bacterium that reduces Cr(VI) to Cr(III), accumulates Pb(II), produces the phytohormone indole-3-acetic acid and siderophores, and increases the plant growth performance of rhizobia in co-inoculation experiments. We aimed to analyze the biotechnological potential of JD2 using a genomic approach. JD2 has a genome of 6.76Mb, with 6,051 predicted protein-coding sequences and 93 RNA genes (tRNA and rRNA). The indole-acetamide pathway was identified as responsible for the synthesis of indole-3-acetic acid. The genetic information involved in chromium resistance (the gene cluster, chrBACF,) was found. At least 40 putative genes encoding for TonB-dependent receptors, probably involved in the utilization of siderophores and biopolymers, and genes for the synthesis, maturation, exportation and uptake of pyoverdine, and acquisition of Fe-pyochelin and Fe-enterobactin were also identified. The information also suggests that JD2 produce polyhydroxy butyrate, a carbon reserve polymer commonly used for manufacturing petrochemical free bioplastics. In addition, JD2 may degrade lignin-derived aromatic compounds to 2-pyrone-4,6-dicarboxylate, a molecule used in the bio-based polymer industry. Finally, a comparative genomic analysis of JD2, Delftia sp. Cs1-4 and Delftia acidovorans SPH-1 is also discussed. The present work provides insights into the physiology and genetics of a microorganism with many potential uses in biotechnology.Agencia Nacional de Investigación e InnovaciónAIMS2019-10-25T14:35:37Z2019-10-25T14:35:37Z2016Artículoinfo:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersion21 p.http://hdl.handle.net/20.500.12381/168FMV_3_2011_1_6089https://doi.org/10.3934/bioeng.2016.2.156AIMS Bioengineering, 3(2): 156-175reponame:IIBCE en REDIinstname:Instituto de Investigaciones Biológicas Clemente Estableinstacron:Instituto de Investigaciones Biológicas Clemente EstableengAcceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND)oai:redi.anii.org.uy:20.500.12381/1682026-06-16T05:25:46Z |
| spellingShingle | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach Morel, María A. Delftia Comparative genomic Plant-microbe interaction Plant-growth promotion Bioremediation Ciencias Naturales y Exactas Ciencias de la Computación e Información Ciencias de la Información y Bioinformática Ciencias Biológicas |
| status_str | publishedVersion |
| title | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| title_full | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| title_fullStr | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| title_full_unstemmed | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| title_short | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| title_sort | Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach |
| topic | Delftia Comparative genomic Plant-microbe interaction Plant-growth promotion Bioremediation Ciencias Naturales y Exactas Ciencias de la Computación e Información Ciencias de la Información y Bioinformática Ciencias Biológicas |
| url | http://hdl.handle.net/20.500.12381/168 https://doi.org/10.3934/bioeng.2016.2.156 |