Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production
The enzymatic hydrolysis of cellulose from Eucalyptus grandis, pretreated through diluted acid impregnation and steam explosion in a continuous pilot-scale reactor, was evaluated for bioethanol production. The best pretreatment conditions were achieved at 180 °C and 0.5 % sulfuric acid, resulting in...
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| مؤلفون آخرون: | , , , , , , , , |
| التنسيق: | article |
| اللغة: | الإنجليزية |
| منشور في: |
2025
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32950 |
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| _version_ | 1868890026578804736 |
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| author | BACQUERIÉ, CAMILA |
| author2 | GUIGOU BERRETTA, MAIRAN DENISE CEBREIROS, FLORENCIA LARNAUDIE PLACHOT, VALERIA INÉS ROMÁN, MARÍA E. CAGNO, MATÍAS RODRÍGUEZ, FACUNDO BONFIGLIO, FERNANDO FERRARI, MARIO DANIEL LAREO, CLAUDIA |
| author2_role | author author author author author author author author author |
| author_browse | BACQUERIÉ, CAMILA BONFIGLIO, FERNANDO CAGNO, MATÍAS CEBREIROS, FLORENCIA FERRARI, MARIO DANIEL GUIGOU BERRETTA, MAIRAN DENISE LAREO, CLAUDIA LARNAUDIE PLACHOT, VALERIA INÉS RODRÍGUEZ, FACUNDO ROMÁN, MARÍA E. |
| author_facet | BACQUERIÉ, CAMILA GUIGOU BERRETTA, MAIRAN DENISE CEBREIROS, FLORENCIA LARNAUDIE PLACHOT, VALERIA INÉS ROMÁN, MARÍA E. CAGNO, MATÍAS RODRÍGUEZ, FACUNDO BONFIGLIO, FERNANDO FERRARI, MARIO DANIEL LAREO, CLAUDIA |
| author_role | author |
| collection | Catálogo digital del LATU |
| dc.coverage.none.fl_str_mv | En: Energy Conversion and Management: X, 27, 101197. DOI: https://doi.org/10.1016/j.ecmx.2025.101197 |
| dc.creator.none.fl_str_mv | BACQUERIÉ, CAMILA GUIGOU BERRETTA, MAIRAN DENISE CEBREIROS, FLORENCIA LARNAUDIE PLACHOT, VALERIA INÉS ROMÁN, MARÍA E. CAGNO, MATÍAS RODRÍGUEZ, FACUNDO BONFIGLIO, FERNANDO FERRARI, MARIO DANIEL LAREO, CLAUDIA |
| dc.date.none.fl_str_mv | 2025-01-01 |
| dc.format.none.fl_str_mv | Pdf |
| dc.identifier.none.fl_str_mv | https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32950 32950 urn:ISBN:69796 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | https://doi.org/10.1016/j.ecmx.2025.101197 info:eu-repo/semantics/openAccess CC BY CC BY |
| dc.source.none.fl_str_mv | reponame:Catálogo digital del LATU instname:Laboratorio Tecnológico del Uruguay instacron:Laboratorio Tecnológico del Uruguay |
| dc.subject.none.fl_str_mv | COMBUSTIBLES ETANOL EUCALYPTUS |
| dc.title.none.fl_str_mv | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article Publicado info:eu-repo/semantics/publishedVersion |
| description | The enzymatic hydrolysis of cellulose from Eucalyptus grandis, pretreated through diluted acid impregnation and steam explosion in a continuous pilot-scale reactor, was evaluated for bioethanol production. The best pretreatment conditions were achieved at 180 °C and 0.5 % sulfuric acid, resulting in an enzymatic hydrolysis efficiency of 61.4 %. The liquid fraction contained up to 89 g/L of xylosaccharides, with a xylan recovery (expressed as xylose) of 50.6 %. The effects of solid content (16–24 % w/w), enzyme dose (15–25 FPU/gglucan), and pH (4.8–6) on enzymatic hydrolysis were assessed using a modified Box-Behnken experimental design. All factors, as well as the interaction between solid content and pH, were significant for achieving high glucose concentrations with high enzymatic hydrolysis efficiency. Higher pH values consistently enhanced hydrolysis efficiency. Multi-objective optimization identified the optimal conditions as pH 6, 24 % solid content, and an enzyme dose of 25 FPU/gglucan, maximizing glucose concentration (122 g/L), enzymatic hydrolysis efficiency (68 %), and glucose yield (49 %). Separate enzymatic hydrolysis and fermentation produced 52 g/L of ethanol, with an overall glucan-to-ethanol conversion efficiency of 46 %. In contrast, when pre-saccharification for 24 h was followed by simultaneous saccharification and fermentation, the performance was lower. Only minimal additional hydrolysis occurred during fermentation. These results contribute to the development of more efficient biomass-to-bioethanol conversion processes, supporting the development of sustainable energy technologies based on lignocellulosic resources. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_467d0486850cf76b3736155ff07b024f |
| identifier_str_mv | 32950 urn:ISBN:69796 |
| instacron_str | Laboratorio Tecnológico del Uruguay |
| institution | Laboratorio Tecnológico del Uruguay |
| instname_str | Laboratorio Tecnológico del Uruguay |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:PMBOAI:32950 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| reponame_str | Catálogo digital del LATU |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | https://doi.org/10.1016/j.ecmx.2025.101197 CC BY CC BY |
| spelling | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol productionBACQUERIÉ, CAMILAGUIGOU BERRETTA, MAIRAN DENISECEBREIROS, FLORENCIALARNAUDIE PLACHOT, VALERIA INÉSROMÁN, MARÍA E.CAGNO, MATÍASRODRÍGUEZ, FACUNDOBONFIGLIO, FERNANDOFERRARI, MARIO DANIELLAREO, CLAUDIACOMBUSTIBLESETANOLEUCALYPTUSThe enzymatic hydrolysis of cellulose from Eucalyptus grandis, pretreated through diluted acid impregnation and steam explosion in a continuous pilot-scale reactor, was evaluated for bioethanol production. The best pretreatment conditions were achieved at 180 °C and 0.5 % sulfuric acid, resulting in an enzymatic hydrolysis efficiency of 61.4 %. The liquid fraction contained up to 89 g/L of xylosaccharides, with a xylan recovery (expressed as xylose) of 50.6 %. The effects of solid content (16–24 % w/w), enzyme dose (15–25 FPU/gglucan), and pH (4.8–6) on enzymatic hydrolysis were assessed using a modified Box-Behnken experimental design. All factors, as well as the interaction between solid content and pH, were significant for achieving high glucose concentrations with high enzymatic hydrolysis efficiency. Higher pH values consistently enhanced hydrolysis efficiency. Multi-objective optimization identified the optimal conditions as pH 6, 24 % solid content, and an enzyme dose of 25 FPU/gglucan, maximizing glucose concentration (122 g/L), enzymatic hydrolysis efficiency (68 %), and glucose yield (49 %). Separate enzymatic hydrolysis and fermentation produced 52 g/L of ethanol, with an overall glucan-to-ethanol conversion efficiency of 46 %. In contrast, when pre-saccharification for 24 h was followed by simultaneous saccharification and fermentation, the performance was lower. Only minimal additional hydrolysis occurred during fermentation. These results contribute to the development of more efficient biomass-to-bioethanol conversion processes, supporting the development of sustainable energy technologies based on lignocellulosic resources. 2025-01-01info:eu-repo/semantics/articlePublicadoinfo:eu-repo/semantics/publishedVersionPdfhttps://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=3295032950urn:ISBN:69796reponame:Catálogo digital del LATUinstname:Laboratorio Tecnológico del Uruguayinstacron:Laboratorio Tecnológico del UruguayengEn: Energy Conversion and Management: X, 27, 101197. DOI: https://doi.org/10.1016/j.ecmx.2025.101197https://doi.org/10.1016/j.ecmx.2025.101197info:eu-repo/semantics/openAccessCC BYCC BYoai:PMBOAI:329502026-06-16T06:18:42Z |
| spellingShingle | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production BACQUERIÉ, CAMILA COMBUSTIBLES ETANOL EUCALYPTUS |
| status_str | publishedVersion |
| title | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| title_full | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| title_fullStr | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| title_full_unstemmed | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| title_short | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| title_sort | Enhanced cellulose enzymatic hydrolysis of pilot-scale steam-exploded Eucalyptus grandis chips with previous acid impregnation for bioethanol production |
| topic | COMBUSTIBLES ETANOL EUCALYPTUS |
| url | https://catalogo.latu.org.uy/opac_css/index.php?lvl=notice_display&id=32950 |