Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma
Angiogenesis is a crucial process in the growth, development, and metastasis of many tumor types, including Non-Hodgkin’s lymphoma (NHL) and Multiple Myeloma (MM). Vascular endothelial growth factor (VEGF) overexpression is known to be associated with poor prognosis in both pathologies, representing...
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| Formaat: | article |
| Taal: | Engels |
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2022
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| Online toegang: | https://hdl.handle.net/20.500.12008/43413 |
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| _version_ | 1868890020428906496 |
|---|---|
| author | Camacho Damata, Ximena |
| author2 | Perroni, Carolina Carneiro, C G Junqueira, M S Machado, C L Faria, Daniele García Melián, María Fernanda Fernández, M Buchpiguel, Carlos Cerecetto, Hugo Chammas, R. Riva, Eloisa Cabral González, Pablo Gambini, Juan Pablo |
| author2_role | author author author author author author author author author author author author author |
| author_browse | Buchpiguel, Carlos Cabral González, Pablo Camacho Damata, Ximena Carneiro, C G Cerecetto, Hugo Chammas, R. Faria, Daniele Fernández, M Gambini, Juan Pablo García Melián, María Fernanda Junqueira, M S Machado, C L Perroni, Carolina Riva, Eloisa |
| author_facet | Camacho Damata, Ximena Perroni, Carolina Carneiro, C G Junqueira, M S Machado, C L Faria, Daniele García Melián, María Fernanda Fernández, M Buchpiguel, Carlos Cerecetto, Hugo Chammas, R. Riva, Eloisa Cabral González, Pablo Gambini, Juan Pablo |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Camacho Damata Ximena, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Perroni Carolina, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Carneiro C G Junqueira M S Machado C L Faria Daniele García Melián María Fernanda, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Fernández M, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Buchpiguel Carlos Cerecetto Hugo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Chammas R. Riva Eloisa, Universidad de la República (Uruguay). Facultad de Medicina. Cabral González Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Gambini Juan Pablo, Universidad de la República (Uruguay). Facultad de Medicina. |
| dc.creator.none.fl_str_mv | Camacho Damata, Ximena Perroni, Carolina Carneiro, C G Junqueira, M S Machado, C L Faria, Daniele García Melián, María Fernanda Fernández, M Buchpiguel, Carlos Cerecetto, Hugo Chammas, R. Riva, Eloisa Cabral González, Pablo Gambini, Juan Pablo |
| dc.date.none.fl_str_mv | 2022 2024-04-11T12:34:04Z 2024-04-11T12:34:04Z |
| dc.format.none.fl_str_mv | 10 h. application/pdf |
| dc.identifier.none.fl_str_mv | Camacho Damata, X, Perroni, C, Carneiro, C [y otros autores]. "Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma". Journal of Molecular Biology and Molecular Imaging. [en línea] 2022, 7(1): 1033. 10 h. 2471-0237 https://hdl.handle.net/20.500.12008/43413 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Austin Publishing Group |
| dc.relation.none.fl_str_mv | Journal of Molecular Biology and Molecular Imaging, 2022, 7(1): 1033. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Bevacizumab Molecular Imaging VEGF Multiple Myeloma Non-Hodgkin Lymphoma 99mTechnetium- or Cy7-lableled Bevacizumab |
| dc.title.none.fl_str_mv | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Angiogenesis is a crucial process in the growth, development, and metastasis of many tumor types, including Non-Hodgkin’s lymphoma (NHL) and Multiple Myeloma (MM). Vascular endothelial growth factor (VEGF) overexpression is known to be associated with poor prognosis in both pathologies, representing a rational target for anti-angiogenic therapy in NHL and MM. The monoclonal antibody Bevacizumab binds to VEGF with high affinity and blocks its action. We aim to evaluate Bevacizumab as a potential radioactive and fluorescence agent for imaging VEGF expression in MM and NHL. Flow cytometry analysis revealed VEGF expression in MM and NHL cell lines is mainly intracellularly. Biodistribution and Single-photon emission computed tomography/computed tomography (SPECT/CT) studies of 99mTc-HYNICBevacizumab showed a slow blood clearance and supradiaphragmatic, head, axial and appendicular skeleton can be evaluated without much interference. Tumor-to-muscle ratio increased with time and is similar to the ones reported with other 99mTc radiolabeled antibodies. Cy7-Bevacizumab fluorescent imaging allowed MM and NHL tumor visualization with greater spatial resolution than SPECT/CT. We successfully synthesized 99mTc and Cy7-labeled anti-VEGF mAb (Bevacizumab) to be used to target VEGF expression in vivo in MM and LNH. Our encouraging results, although working with 99mTc, highlight the importance of radioinmuno-oncology as a potential tool to fight these diseases. Optical imaging of these tracers would enhance tumor sampling and guide surgical removal. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_41dddcbd5fb8da952481ea4145fefc67 |
| identifier_str_mv | Camacho Damata, X, Perroni, C, Carneiro, C [y otros autores]. "Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma". Journal of Molecular Biology and Molecular Imaging. [en línea] 2022, 7(1): 1033. 10 h. 2471-0237 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/43413 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher.none.fl_str_mv | Austin Publishing Group |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin LymphomaCamacho Damata, XimenaPerroni, CarolinaCarneiro, C GJunqueira, M SMachado, C LFaria, DanieleGarcía Melián, María FernandaFernández, MBuchpiguel, CarlosCerecetto, HugoChammas, R.Riva, EloisaCabral González, PabloGambini, Juan PabloBevacizumabMolecular ImagingVEGFMultiple MyelomaNon-Hodgkin Lymphoma99mTechnetium- or Cy7-lableled BevacizumabAngiogenesis is a crucial process in the growth, development, and metastasis of many tumor types, including Non-Hodgkin’s lymphoma (NHL) and Multiple Myeloma (MM). Vascular endothelial growth factor (VEGF) overexpression is known to be associated with poor prognosis in both pathologies, representing a rational target for anti-angiogenic therapy in NHL and MM. The monoclonal antibody Bevacizumab binds to VEGF with high affinity and blocks its action. We aim to evaluate Bevacizumab as a potential radioactive and fluorescence agent for imaging VEGF expression in MM and NHL. Flow cytometry analysis revealed VEGF expression in MM and NHL cell lines is mainly intracellularly. Biodistribution and Single-photon emission computed tomography/computed tomography (SPECT/CT) studies of 99mTc-HYNICBevacizumab showed a slow blood clearance and supradiaphragmatic, head, axial and appendicular skeleton can be evaluated without much interference. Tumor-to-muscle ratio increased with time and is similar to the ones reported with other 99mTc radiolabeled antibodies. Cy7-Bevacizumab fluorescent imaging allowed MM and NHL tumor visualization with greater spatial resolution than SPECT/CT. We successfully synthesized 99mTc and Cy7-labeled anti-VEGF mAb (Bevacizumab) to be used to target VEGF expression in vivo in MM and LNH. Our encouraging results, although working with 99mTc, highlight the importance of radioinmuno-oncology as a potential tool to fight these diseases. Optical imaging of these tracers would enhance tumor sampling and guide surgical removal.ANII: POS_NAC_2015_1_109490CSIC: 240600-000148-18Austin Publishing GroupCamacho Damata Ximena, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Perroni Carolina, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Carneiro C GJunqueira M SMachado C LFaria DanieleGarcía Melián María Fernanda, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Fernández M, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Buchpiguel CarlosCerecetto Hugo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Chammas R.Riva Eloisa, Universidad de la República (Uruguay). Facultad de Medicina.Cabral González Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Gambini Juan Pablo, Universidad de la República (Uruguay). Facultad de Medicina.2024-04-11T12:34:04Z2024-04-11T12:34:04Z2022Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 h.application/pdfCamacho Damata, X, Perroni, C, Carneiro, C [y otros autores]. "Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma". Journal of Molecular Biology and Molecular Imaging. [en línea] 2022, 7(1): 1033. 10 h.2471-0237https://hdl.handle.net/20.500.12008/43413reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Molecular Biology and Molecular Imaging, 2022, 7(1): 1033.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/434132026-04-14T10:10:38Z |
| spellingShingle | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma Camacho Damata, Ximena Bevacizumab Molecular Imaging VEGF Multiple Myeloma Non-Hodgkin Lymphoma 99mTechnetium- or Cy7-lableled Bevacizumab |
| status_str | publishedVersion |
| title | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| title_full | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| title_fullStr | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| title_full_unstemmed | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| title_short | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| title_sort | Molecular imaging of VEGF expression in multiple myeloma and non-Hodgkin Lymphoma |
| topic | Bevacizumab Molecular Imaging VEGF Multiple Myeloma Non-Hodgkin Lymphoma 99mTechnetium- or Cy7-lableled Bevacizumab |
| url | https://hdl.handle.net/20.500.12008/43413 |