The traceability of Eucalyptus clones using molecular markers.
ABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced f...
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| Language: | English |
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2021
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| Online Access: | https://ainfo.inia.uy/consulta/busca?b=pc&id=62729&biblioteca=vazio&busca=62729&qFacets=62729 |
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| _version_ | 1868890016737918976 |
|---|---|
| author | TORRES, D. |
| author2 | DELGADO-CERRONE, L. LUNA, L. RESQUÍN, F. AGUILAR, A.V. SEBENN, A.M. |
| author2_role | author author author author author |
| author_browse | AGUILAR, A.V. DELGADO-CERRONE, L. LUNA, L. RESQUÍN, F. SEBENN, A.M. TORRES, D. |
| author_facet | TORRES, D. DELGADO-CERRONE, L. LUNA, L. RESQUÍN, F. AGUILAR, A.V. SEBENN, A.M. |
| author_role | author |
| collection | AINFO |
| dc.creator.none.fl_str_mv | TORRES, D. DELGADO-CERRONE, L. LUNA, L. RESQUÍN, F. AGUILAR, A.V. SEBENN, A.M. |
| dc.date.none.fl_str_mv | 2021 2025-06-23T18:23:10Z 2025-06-23T18:23:10Z 2025-06-23T18:23:10Z |
| dc.identifier.none.fl_str_mv | https://ainfo.inia.uy/consulta/busca?b=pc&id=62729&biblioteca=vazio&busca=62729&qFacets=62729 |
| dc.language.none.fl_str_mv | en eng |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Acceso abierto |
| dc.source.none.fl_str_mv | reponame:AINFO instname:Instituto Nacional de Investigación Agropecuaria instacron:Instituto Nacional de Investigación Agropecuaria |
| dc.subject.none.fl_str_mv | Clone certification Clone Genotyping Identity Multiplex Nurseries Traceability |
| dc.title.none.fl_str_mv | The traceability of Eucalyptus clones using molecular markers. |
| dc.type.none.fl_str_mv | Article PublishedVersion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | ABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.© 2020 Diego Torres-Dini et al., published by Sciendo |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_3eb0876ae2ff3767a656a73e4121ffce |
| instacron_str | Instituto Nacional de Investigación Agropecuaria |
| institution | Instituto Nacional de Investigación Agropecuaria |
| instname_str | Instituto Nacional de Investigación Agropecuaria |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/4186 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | AINFO |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto |
| spelling | The traceability of Eucalyptus clones using molecular markers.TORRES, D.DELGADO-CERRONE, L.LUNA, L.RESQUÍN, F.AGUILAR, A.V.SEBENN, A.M.Clone certificationCloneGenotypingIdentityMultiplexNurseriesTraceabilityABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.© 2020 Diego Torres-Dini et al., published by Sciendo2025-06-23T18:23:10Z2025-06-23T18:23:10Z20212025-06-23T18:23:10ZArticlePublishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://ainfo.inia.uy/consulta/busca?b=pc&id=62729&biblioteca=vazio&busca=62729&qFacets=62729reponame:AINFOinstname:Instituto Nacional de Investigación Agropecuariainstacron:Instituto Nacional de Investigación Agropecuariaenenginfo:eu-repo/semantics/openAccessAcceso abiertooai:redi.anii.org.uy:20.500.12381/41862026-02-10T17:37:02Z |
| spellingShingle | The traceability of Eucalyptus clones using molecular markers. TORRES, D. Clone certification Clone Genotyping Identity Multiplex Nurseries Traceability |
| status_str | publishedVersion |
| title | The traceability of Eucalyptus clones using molecular markers. |
| title_full | The traceability of Eucalyptus clones using molecular markers. |
| title_fullStr | The traceability of Eucalyptus clones using molecular markers. |
| title_full_unstemmed | The traceability of Eucalyptus clones using molecular markers. |
| title_short | The traceability of Eucalyptus clones using molecular markers. |
| title_sort | The traceability of Eucalyptus clones using molecular markers. |
| topic | Clone certification Clone Genotyping Identity Multiplex Nurseries Traceability |
| url | https://ainfo.inia.uy/consulta/busca?b=pc&id=62729&biblioteca=vazio&busca=62729&qFacets=62729 |