An ascomycete H4 variant with an unknown function
Histone variants leading to altered nucleosome structure, dynamics and DNA accessibility occur frequently, albeit rarely for H4. We carried out a comprehensive in silico scrutiny of fungal genomes, which revealed the presence of a novel H4 variant (H4E) in the ascomycetes, throughout the Pezizomycot...
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| Другие авторы: | , , , , |
| Формат: | article |
| Язык: | английский |
| Опубликовано: |
2024
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| Предметы: | |
| Online-ссылка: | https://hdl.handle.net/20.500.12008/50268 |
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| _version_ | 1868890012547809280 |
|---|---|
| author | Flipphi, Michel |
| author2 | Harispe, María Laura Hamari, Zsuzsanna Kocsubé, Sándor Scazzocchio, Claudio Ramón, Ana |
| author2_role | author author author author author |
| author_browse | Flipphi, Michel Hamari, Zsuzsanna Harispe, María Laura Kocsubé, Sándor Ramón, Ana Scazzocchio, Claudio |
| author_facet | Flipphi, Michel Harispe, María Laura Hamari, Zsuzsanna Kocsubé, Sándor Scazzocchio, Claudio Ramón, Ana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Flipphi Michel Harispe María Laura, ANEP Hamari Zsuzsanna Kocsubé Sándor Scazzocchio Claudio Ramón Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. |
| dc.creator.none.fl_str_mv | Flipphi, Michel Harispe, María Laura Hamari, Zsuzsanna Kocsubé, Sándor Scazzocchio, Claudio Ramón, Ana |
| dc.date.none.fl_str_mv | 2024 2025-06-11T16:26:27Z 2025-06-11T16:26:27Z |
| dc.format.none.fl_str_mv | 16 h application/pdf |
| dc.identifier.none.fl_str_mv | Flipphi, M, Harispe, M, Hamari, Z [y otros autores]. "An ascomycete H4 variant with an unknown function". Royal Society Open Science. [en línea] 2024, 11(2): 231705. 16 h. DOI: 10.1098/rsos.231705 2054-5703 https://hdl.handle.net/20.500.12008/50268 10.1098/rsos.231705 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | The Royal Society |
| dc.relation.none.fl_str_mv | Royal Society Open Science, 2024, 11(2): 231705. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Histone H4 variant Chromatin Ascomycetes |
| dc.title.none.fl_str_mv | An ascomycete H4 variant with an unknown function |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Histone variants leading to altered nucleosome structure, dynamics and DNA accessibility occur frequently, albeit rarely for H4. We carried out a comprehensive in silico scrutiny of fungal genomes, which revealed the presence of a novel H4 variant (H4E) in the ascomycetes, throughout the Pezizomycotina, in basal species of the Taphrinomycotina and also in the Glomeromycota. The coding cognate genes show a specific intron/exon organization, different from H4 canonical genes. H4Es diverge from canonical H4s mainly in the N- and C-terminal extensions, showing marked differences in the distribution and number of Lys and Arg residues, which may result in novel post-translational modifications. In Aspergillus nidulans (Pezizomycotina, Eurotiomycetes) the H4E variant protein level is low in mycelia. However, the encoding gene is well expressed at 37°C under nitrogen starvation. H4E localizes to the nucleus and interacts with H3, but its absence or overexpression does not result in any detectable phenotype. Deletion of only one of the of the two canonical H4 genes results in a strikingly impaired growth phenotype, which indicates that H4E cannot replace this canonical histone. Thus, an H4 variant is present throughout a whole subphylum of the ascomycetes, but with hitherto no experimentally detectable function. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_3a891b3cb5cbb2b76c3cc8469ab8af89 |
| identifier_str_mv | Flipphi, M, Harispe, M, Hamari, Z [y otros autores]. "An ascomycete H4 variant with an unknown function". Royal Society Open Science. [en línea] 2024, 11(2): 231705. 16 h. DOI: 10.1098/rsos.231705 2054-5703 10.1098/rsos.231705 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50268 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher.none.fl_str_mv | The Royal Society |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | An ascomycete H4 variant with an unknown functionFlipphi, MichelHarispe, María LauraHamari, ZsuzsannaKocsubé, SándorScazzocchio, ClaudioRamón, AnaHistone H4 variantChromatinAscomycetesHistone variants leading to altered nucleosome structure, dynamics and DNA accessibility occur frequently, albeit rarely for H4. We carried out a comprehensive in silico scrutiny of fungal genomes, which revealed the presence of a novel H4 variant (H4E) in the ascomycetes, throughout the Pezizomycotina, in basal species of the Taphrinomycotina and also in the Glomeromycota. The coding cognate genes show a specific intron/exon organization, different from H4 canonical genes. H4Es diverge from canonical H4s mainly in the N- and C-terminal extensions, showing marked differences in the distribution and number of Lys and Arg residues, which may result in novel post-translational modifications. In Aspergillus nidulans (Pezizomycotina, Eurotiomycetes) the H4E variant protein level is low in mycelia. However, the encoding gene is well expressed at 37°C under nitrogen starvation. H4E localizes to the nucleus and interacts with H3, but its absence or overexpression does not result in any detectable phenotype. Deletion of only one of the of the two canonical H4 genes results in a strikingly impaired growth phenotype, which indicates that H4E cannot replace this canonical histone. Thus, an H4 variant is present throughout a whole subphylum of the ascomycetes, but with hitherto no experimentally detectable function.The Royal SocietyFlipphi MichelHarispe María Laura, ANEPHamari ZsuzsannaKocsubé SándorScazzocchio ClaudioRamón Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.2025-06-11T16:26:27Z2025-06-11T16:26:27Z2024Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 happlication/pdfFlipphi, M, Harispe, M, Hamari, Z [y otros autores]. "An ascomycete H4 variant with an unknown function". Royal Society Open Science. [en línea] 2024, 11(2): 231705. 16 h. DOI: 10.1098/rsos.2317052054-5703https://hdl.handle.net/20.500.12008/5026810.1098/rsos.231705reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengRoyal Society Open Science, 2024, 11(2): 231705.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/502682026-04-14T10:10:51Z |
| spellingShingle | An ascomycete H4 variant with an unknown function Flipphi, Michel Histone H4 variant Chromatin Ascomycetes |
| status_str | publishedVersion |
| title | An ascomycete H4 variant with an unknown function |
| title_full | An ascomycete H4 variant with an unknown function |
| title_fullStr | An ascomycete H4 variant with an unknown function |
| title_full_unstemmed | An ascomycete H4 variant with an unknown function |
| title_short | An ascomycete H4 variant with an unknown function |
| title_sort | An ascomycete H4 variant with an unknown function |
| topic | Histone H4 variant Chromatin Ascomycetes |
| url | https://hdl.handle.net/20.500.12008/50268 |