Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems

The ability to perceive the environment, an essential attribute in living organisms, is linked to the evolution of signalling proteins that recognize specific signals and execute predetermined responses. Such proteins constitute concerted systems that can be as simple as a unique protein, able to re...

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Hovedforfatter: Trajtenberg, Felipe (author)
Andre forfattere: Buschiazzo, Alejandro (author)
Format: bookPart
Sprog:engelsk
Udgivet: 2020
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Online adgang:https://hdl.handle.net/20.500.12381/602
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author Trajtenberg, Felipe
author2 Buschiazzo, Alejandro
author2_role author
author_browse Buschiazzo, Alejandro
Trajtenberg, Felipe
author_facet Trajtenberg, Felipe
Buschiazzo, Alejandro
author_role author
collection IPMON en REDI
dc.creator.none.fl_str_mv Trajtenberg, Felipe
Buschiazzo, Alejandro
dc.date.none.fl_str_mv 2020
2022-07-01T14:02:33Z
2022-07-01T14:02:33Z
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12381/602
FCE_1_2017_1_136291
10.1007/978-1-4939-9884-5_1
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Springer Nature (Humana Press)
dc.relation.none.fl_str_mv https://hdl.handle.net/20.500.12381/603
https://hdl.handle.net/20.500.12381/604
dc.rights.none.fl_str_mv Acceso abierto
info:eu-repo/semantics/openAccess
Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND)
dc.source.none.fl_str_mv Histidine Phosphorylation - Methods and Protocols
reponame:IPMON en REDI
instname:Institut Pasteur de Montevideo
instacron:Institut Pasteur de Montevideo
dc.subject.none.fl_str_mv señalización bacteriana
Fosforilación de proteínas
Alosterismo
Histidin-quinasa
Regulador de respuesta
Ciencias Naturales y Exactas
Ciencias Biológicas
Bioquímica y Biología Molecular
dc.title.none.fl_str_mv Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
dc.type.none.fl_str_mv Parte de libro
info:eu-repo/semantics/bookPart
Aceptado
info:eu-repo/semantics/acceptedVersion
description The ability to perceive the environment, an essential attribute in living organisms, is linked to the evolution of signalling proteins that recognize specific signals and execute predetermined responses. Such proteins constitute concerted systems that can be as simple as a unique protein, able to recognize a ligand and exert a phenotypic change, or extremely complex pathways engaging dozens of different proteins which act in coordination with feedback loops and signal modulation. To understand how cells sense their surroundings and mount specific adaptive responses, we need to decipher the molecular workings of signal recognition, internalization, transfer and conversion into chemical changes inside the cell. Protein allostery and dynamics play a central role. Here, we review recent progress on the study of two- component systems, important signalling machineries of prokaryotes and lower eukaryotes. Such systems implicate a sensory histidine-kinase and a separate response regulator protein. Both components exploit protein flexibility to effect specific conformational rearrangements, modulating protein:protein interactions, and ultimately transmitting information accurately. Recent work has revealed how histidine-kinases switch between discrete functional states according to the presence or absence of the signal, shifting key amino acid positions that define their catalytic activity. In concert with the cognate response regulator’s allosteric changes, the phosphoryl-transfer flow during the signalling process is exquisitely fine-tuned for proper specificity, efficiency and directionality.
eu_rights_str_mv openAccess
format bookPart
id anni_3987f0f1b26a2ca36e33d1d89f1a74cd
identifier_str_mv FCE_1_2017_1_136291
10.1007/978-1-4939-9884-5_1
instacron_str Institut Pasteur de Montevideo
institution Institut Pasteur de Montevideo
instname_str Institut Pasteur de Montevideo
language eng
network_acronym_str anni
network_name_str oai-lr-anni
oai_identifier_str oai:redi.anii.org.uy:20.500.12381/602
publishDate 2020
publishDateSort 2020
publisher.none.fl_str_mv Springer Nature (Humana Press)
reponame_str IPMON en REDI
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv Acceso abierto
Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND)
spelling Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component SystemsTrajtenberg, FelipeBuschiazzo, Alejandroseñalización bacterianaFosforilación de proteínasAlosterismoHistidin-quinasaRegulador de respuestaCiencias Naturales y ExactasCiencias BiológicasBioquímica y Biología MolecularThe ability to perceive the environment, an essential attribute in living organisms, is linked to the evolution of signalling proteins that recognize specific signals and execute predetermined responses. Such proteins constitute concerted systems that can be as simple as a unique protein, able to recognize a ligand and exert a phenotypic change, or extremely complex pathways engaging dozens of different proteins which act in coordination with feedback loops and signal modulation. To understand how cells sense their surroundings and mount specific adaptive responses, we need to decipher the molecular workings of signal recognition, internalization, transfer and conversion into chemical changes inside the cell. Protein allostery and dynamics play a central role. Here, we review recent progress on the study of two- component systems, important signalling machineries of prokaryotes and lower eukaryotes. Such systems implicate a sensory histidine-kinase and a separate response regulator protein. Both components exploit protein flexibility to effect specific conformational rearrangements, modulating protein:protein interactions, and ultimately transmitting information accurately. Recent work has revealed how histidine-kinases switch between discrete functional states according to the presence or absence of the signal, shifting key amino acid positions that define their catalytic activity. In concert with the cognate response regulator’s allosteric changes, the phosphoryl-transfer flow during the signalling process is exquisitely fine-tuned for proper specificity, efficiency and directionality.Agencia Nacional de Investigación e InnovaciónSpringer Nature (Humana Press)2022-07-01T14:02:33Z2022-07-01T14:02:33Z2020Parte de libroinfo:eu-repo/semantics/bookPartAceptadoinfo:eu-repo/semantics/acceptedVersionhttps://hdl.handle.net/20.500.12381/602FCE_1_2017_1_13629110.1007/978-1-4939-9884-5_1Histidine Phosphorylation - Methods and Protocolsreponame:IPMON en REDIinstname:Institut Pasteur de Montevideoinstacron:Institut Pasteur de Montevideoenghttps://hdl.handle.net/20.500.12381/603https://hdl.handle.net/20.500.12381/604Acceso abiertoinfo:eu-repo/semantics/openAccessReconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. (CC BY-NC-ND)oai:redi.anii.org.uy:20.500.12381/6022026-06-16T05:20:27Z
spellingShingle Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
Trajtenberg, Felipe
señalización bacteriana
Fosforilación de proteínas
Alosterismo
Histidin-quinasa
Regulador de respuesta
Ciencias Naturales y Exactas
Ciencias Biológicas
Bioquímica y Biología Molecular
status_str acceptedVersion
title Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
title_full Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
title_fullStr Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
title_full_unstemmed Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
title_short Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
title_sort Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems
topic señalización bacteriana
Fosforilación de proteínas
Alosterismo
Histidin-quinasa
Regulador de respuesta
Ciencias Naturales y Exactas
Ciencias Biológicas
Bioquímica y Biología Molecular
url https://hdl.handle.net/20.500.12381/602