Detection and quantification of nitric oxide–derived oxidants in biological systems
The free radical nitric oxide (NO) exerts biological effects through the direct and reversible interaction with specific targets (e.g. soluble guanylate cyclase) or through the generation of secondary species, many of which can oxidize, nitrosate or nitrate biomolecules. The NO -derived reactive spe...
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| Other Authors: | , , , , |
| Format: | article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/20.500.12008/26647 |
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| _version_ | 1868889999635644416 |
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| author | Möller, Matías N. |
| author2 | Ríos, Natalia Trujillo, Madia Radi, Rafael Denicola, Ana Álvarez, Beatriz |
| author2_role | author author author author author |
| author_browse | Denicola, Ana Möller, Matías N. Radi, Rafael Ríos, Natalia Trujillo, Madia Álvarez, Beatriz |
| author_facet | Möller, Matías N. Ríos, Natalia Trujillo, Madia Radi, Rafael Denicola, Ana Álvarez, Beatriz |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Möller Matías N, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Ríos Natalia, Universidad de la República (Uruguay). Facultad de Medicina. Trujillo Madia, Universidad de la República (Uruguay). Facultad de Medicina. Radi Rafael, Universidad de la República (Uruguay). Facultad de Medicina. Denicola Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Alvarez Beatriz, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. |
| dc.creator.none.fl_str_mv | Möller, Matías N. Ríos, Natalia Trujillo, Madia Radi, Rafael Denicola, Ana Álvarez, Beatriz |
| dc.date.none.fl_str_mv | 2019 2021-03-01T16:08:45Z 2021-03-01T16:08:45Z |
| dc.format.none.fl_str_mv | 27 h application/pdf |
| dc.identifier.none.fl_str_mv | Möller, M, Ríos, N, Trujillo, y otros. "Detection and quantification of nitric oxide–derived oxidants in biological systems". Journal of Biological Chemistry. [en línea] , 2019, 294(40):14776-14802. 27 h. DOI: 10.1074/jbc.REV119.006136 0021-9258 https://hdl.handle.net/20.500.12008/26647 10.1074/jbc.REV119.006136 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | American Society for Biochemistry and Molecular Biology |
| dc.relation.none.fl_str_mv | Journal of Biological Chemistry, 2019, 294(40):14776-14802 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | 3-nitrotyrosine Nitration Nitric oxide Nitrogen dioxide Nitrosation Nitrosative stress Nitrosylation Oxidation–reduction (redox) Oxidative stress Oxygen radicals Peroxynitrite Reactive nitrogen species Reactive oxygen species |
| dc.title.none.fl_str_mv | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The free radical nitric oxide (NO) exerts biological effects through the direct and reversible interaction with specific targets (e.g. soluble guanylate cyclase) or through the generation of secondary species, many of which can oxidize, nitrosate or nitrate biomolecules. The NO -derived reactive species are typically short-lived, and their preferential fates depend on kinetic and compartmentalization aspects. Their detection and quantification are technically challenging. In general, the strategies employed are based either on the detection of relatively stable end products or on the use of synthetic probes, and they are not always selective for a particular species. In this study, we describe the biologically relevant characteristics of the reactive species formed downstream from NO , and we discuss the approaches currently available for the analysis of NO , nitrogen dioxide (NO2 ), dinitrogen trioxide (N2O3), nitroxyl (HNO), and peroxynitrite (ONOO /ONOOH), as well as peroxynitrite-derived hydroxyl (HO) and carbonate anion (CO3) radicals. We also discuss the biological origins of and analytical tools for detecting nitrite (NO2), nitrate (NO3), nitrosyl–metal complexes, S-nitrosothiols, and 3-nitrotyrosine. Moreover, we highlight state– of–the–art methods, alert readers to caveats of widely used techniques, and encourage retirement of approaches that have been supplanted by more reliable and selective tools for detecting and measuring NO -derived oxidants. We emphasize that the use of appropriate analytical methods needs to be strongly grounded in a chemical and biochemical understanding of the species and mechanistic pathways involve |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_30b446f271748fbcfd76d8a264e8c2da |
| identifier_str_mv | Möller, M, Ríos, N, Trujillo, y otros. "Detection and quantification of nitric oxide–derived oxidants in biological systems". Journal of Biological Chemistry. [en línea] , 2019, 294(40):14776-14802. 27 h. DOI: 10.1074/jbc.REV119.006136 0021-9258 10.1074/jbc.REV119.006136 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/26647 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | American Society for Biochemistry and Molecular Biology |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Detection and quantification of nitric oxide–derived oxidants in biological systemsMöller, Matías N.Ríos, NataliaTrujillo, MadiaRadi, RafaelDenicola, AnaÁlvarez, Beatriz3-nitrotyrosineNitrationNitric oxideNitrogen dioxideNitrosationNitrosative stressNitrosylationOxidation–reduction (redox)Oxidative stressOxygen radicalsPeroxynitriteReactive nitrogen speciesReactive oxygen speciesThe free radical nitric oxide (NO) exerts biological effects through the direct and reversible interaction with specific targets (e.g. soluble guanylate cyclase) or through the generation of secondary species, many of which can oxidize, nitrosate or nitrate biomolecules. The NO -derived reactive species are typically short-lived, and their preferential fates depend on kinetic and compartmentalization aspects. Their detection and quantification are technically challenging. In general, the strategies employed are based either on the detection of relatively stable end products or on the use of synthetic probes, and they are not always selective for a particular species. In this study, we describe the biologically relevant characteristics of the reactive species formed downstream from NO , and we discuss the approaches currently available for the analysis of NO , nitrogen dioxide (NO2 ), dinitrogen trioxide (N2O3), nitroxyl (HNO), and peroxynitrite (ONOO /ONOOH), as well as peroxynitrite-derived hydroxyl (HO) and carbonate anion (CO3) radicals. We also discuss the biological origins of and analytical tools for detecting nitrite (NO2), nitrate (NO3), nitrosyl–metal complexes, S-nitrosothiols, and 3-nitrotyrosine. Moreover, we highlight state– of–the–art methods, alert readers to caveats of widely used techniques, and encourage retirement of approaches that have been supplanted by more reliable and selective tools for detecting and measuring NO -derived oxidants. We emphasize that the use of appropriate analytical methods needs to be strongly grounded in a chemical and biochemical understanding of the species and mechanistic pathways involveAgencia Nacional de Investigación e Innovación FCE_1_2017_1_136043Comisión Sectorial de Investigación Científica (CSIC)Universidad de la República. Espacio InterdisciplinarioAmerican Society for Biochemistry and Molecular BiologyMöller Matías N, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Ríos Natalia, Universidad de la República (Uruguay). Facultad de Medicina.Trujillo Madia, Universidad de la República (Uruguay). Facultad de Medicina.Radi Rafael, Universidad de la República (Uruguay). Facultad de Medicina.Denicola Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Alvarez Beatriz, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.2021-03-01T16:08:45Z2021-03-01T16:08:45Z2019Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion27 happlication/pdfMöller, M, Ríos, N, Trujillo, y otros. "Detection and quantification of nitric oxide–derived oxidants in biological systems". Journal of Biological Chemistry. [en línea] , 2019, 294(40):14776-14802. 27 h. DOI: 10.1074/jbc.REV119.0061360021-9258https://hdl.handle.net/20.500.12008/2664710.1074/jbc.REV119.006136reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Biological Chemistry, 2019, 294(40):14776-14802Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/266472026-04-14T10:09:31Z |
| spellingShingle | Detection and quantification of nitric oxide–derived oxidants in biological systems Möller, Matías N. 3-nitrotyrosine Nitration Nitric oxide Nitrogen dioxide Nitrosation Nitrosative stress Nitrosylation Oxidation–reduction (redox) Oxidative stress Oxygen radicals Peroxynitrite Reactive nitrogen species Reactive oxygen species |
| status_str | publishedVersion |
| title | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| title_full | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| title_fullStr | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| title_full_unstemmed | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| title_short | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| title_sort | Detection and quantification of nitric oxide–derived oxidants in biological systems |
| topic | 3-nitrotyrosine Nitration Nitric oxide Nitrogen dioxide Nitrosation Nitrosative stress Nitrosylation Oxidation–reduction (redox) Oxidative stress Oxygen radicals Peroxynitrite Reactive nitrogen species Reactive oxygen species |
| url | https://hdl.handle.net/20.500.12008/26647 |