Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels
An extremely simple, inexpensive, and safe method is presented, which emulates nucleic acids isolation and electrophoretic analysis as performed in a research environment, in the context of a secondary school hands-on activity. The protocol is amenable to an interdisciplinary approach, taking into c...
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| Other Authors: | , |
| Format: | article |
| Language: | English |
| Published: |
2004
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| Online Access: | http://repositorio.cfe.edu.uy/handle/123456789/1235 |
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| _version_ | 1868889988309975040 |
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| author | Britos, Leticia |
| author2 | Goyenola, Guillermo Umpiérrez Oroño, Silvia |
| author2_role | author author |
| author_browse | Britos, Leticia Goyenola, Guillermo Umpiérrez Oroño, Silvia |
| author_facet | Britos, Leticia Goyenola, Guillermo Umpiérrez Oroño, Silvia |
| author_role | author |
| collection | RIdAA-CFE |
| dc.creator.none.fl_str_mv | Britos, Leticia Goyenola, Guillermo Umpiérrez Oroño, Silvia |
| dc.date.none.fl_str_mv | 2004 2021-02-05T12:49:42Z 2021-02-05T12:49:42Z 2021-02-05 |
| dc.format.none.fl_str_mv | pdf pp. 341-347 |
| dc.identifier.none.fl_str_mv | http://repositorio.cfe.edu.uy/handle/123456789/1235 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Unión Internacional de Bioquímica y Biología Molecular |
| dc.relation.none.fl_str_mv | Biochemistry and molecular biology education |
| dc.rights.none.fl_str_mv | openAccess info:eu-repo/semantics/openAccess cc by-nc 4.0 |
| dc.source.none.fl_str_mv | Biochemistry and molecular biology education; Volumen 32, Número 5 (2004) reponame:RIdAA-CFE instname:ANEP. Consejo de Formación en Educación instacron:ANEP. Consejo de Formación en Educación |
| dc.subject.none.fl_str_mv | genoma bioquímica Enseñanza secundaria Prácticas de laboratorio Biología molecular electroforesis |
| dc.title.none.fl_str_mv | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article published info:eu-repo/semantics/publishedVersion |
| description | An extremely simple, inexpensive, and safe method is presented, which emulates nucleic acids isolation and electrophoretic analysis as performed in a research environment, in the context of a secondary school hands-on activity. The protocol is amenable to an interdisciplinary approach, taking into consideration the electrical and chemical parameters of the electrophoretic system. Furthermore, the laboratory is framed in a more comprehensive pedagogical setting, which addresses the methodological aspects of a pivotal scientific enterprise such as the Human Genome Project. In this setting, the hands-on activity is complemented with animations, paper models, and discussions. Additionally, our results indicate that the use of borate buffer and agar-agar gels suits many of the experiments included in college-level laboratory activities, which currently make use of more expensive agarose gels and TBE or TAE buffers |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_281e0caa057aad6fc8917aa2fb3b68e5 |
| instacron_str | ANEP. Consejo de Formación en Educación |
| institution | ANEP. Consejo de Formación en Educación |
| instname_str | ANEP. Consejo de Formación en Educación |
| language | eng |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:repositorio.cfe.edu.uy:123456789/1235 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher.none.fl_str_mv | Unión Internacional de Bioquímica y Biología Molecular |
| reponame_str | RIdAA-CFE |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | openAccess cc by-nc 4.0 |
| spelling | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate GelsBritos, LeticiaGoyenola, GuillermoUmpiérrez Oroño, SilviagenomabioquímicaEnseñanza secundariaPrácticas de laboratorioBiología molecularelectroforesisAn extremely simple, inexpensive, and safe method is presented, which emulates nucleic acids isolation and electrophoretic analysis as performed in a research environment, in the context of a secondary school hands-on activity. The protocol is amenable to an interdisciplinary approach, taking into consideration the electrical and chemical parameters of the electrophoretic system. Furthermore, the laboratory is framed in a more comprehensive pedagogical setting, which addresses the methodological aspects of a pivotal scientific enterprise such as the Human Genome Project. In this setting, the hands-on activity is complemented with animations, paper models, and discussions. Additionally, our results indicate that the use of borate buffer and agar-agar gels suits many of the experiments included in college-level laboratory activities, which currently make use of more expensive agarose gels and TBE or TAE buffersUnión Internacional de Bioquímica y Biología Molecular2021-02-05T12:49:42Z2021-02-05T12:49:42Z20042021-02-05info:eu-repo/semantics/articlepublishedinfo:eu-repo/semantics/publishedVersionpdfpp. 341-347http://repositorio.cfe.edu.uy/handle/123456789/1235Biochemistry and molecular biology education; Volumen 32, Número 5 (2004)reponame:RIdAA-CFEinstname:ANEP. Consejo de Formación en Educacióninstacron:ANEP. Consejo de Formación en EducaciónengBiochemistry and molecular biology educationopenAccessinfo:eu-repo/semantics/openAccesscc by-nc 4.0oai:repositorio.cfe.edu.uy:123456789/12352026-06-16T06:04:38Z |
| spellingShingle | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels Britos, Leticia genoma bioquímica Enseñanza secundaria Prácticas de laboratorio Biología molecular electroforesis |
| status_str | publishedVersion |
| title | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| title_full | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| title_fullStr | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| title_full_unstemmed | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| title_short | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| title_sort | Simple Protocol for Secondary School Hands-On Activity: Electrophoresis of Pre-Stained Nucleic Acids on Agar-Agar Borate Gels |
| topic | genoma bioquímica Enseñanza secundaria Prácticas de laboratorio Biología molecular electroforesis |
| url | http://repositorio.cfe.edu.uy/handle/123456789/1235 |