Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins wer...
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| Format: | article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/20.500.12008/43074 https://doi.org/10.3168/jds.2021-21358 |
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| _version_ | 1868889952960380928 |
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| author | García-Roche Saracco, María Mercedes |
| author2 | Talmón Travers, Daniel Cañibe, Guillermo Astessiano, Ana Laura Mendoza, Alejandro Quijano, Daniel Cassina, Adriana Carriquiry Fossemale, Mariana |
| author2_role | author author author author author author author |
| author_browse | Astessiano, Ana Laura Carriquiry Fossemale, Mariana Cassina, Adriana Cañibe, Guillermo García-Roche Saracco, María Mercedes Mendoza, Alejandro Quijano, Daniel Talmón Travers, Daniel |
| author_facet | García-Roche Saracco, María Mercedes Talmón Travers, Daniel Cañibe, Guillermo Astessiano, Ana Laura Mendoza, Alejandro Quijano, Daniel Cassina, Adriana Carriquiry Fossemale, Mariana |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | García-Roche Saracco María Mercedes Talmón Travers Daniel Cañibe Guillermo Astessiano Ana Laura Mendoza Alejandro Quijano Daniel Cassina Adriana Carriquiry Fossemale Mariana |
| dc.creator.none.fl_str_mv | García-Roche Saracco, María Mercedes Talmón Travers, Daniel Cañibe, Guillermo Astessiano, Ana Laura Mendoza, Alejandro Quijano, Daniel Cassina, Adriana Carriquiry Fossemale, Mariana |
| dc.date.none.fl_str_mv | 2022 2024-03-14T12:30:59Z 2024-03-14T12:30:59Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | García-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737 https://hdl.handle.net/20.500.12008/43074 https://doi.org/10.3168/jds.2021-21358 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.relation.none.fl_str_mv | Journal of Dairy Science, 105(7): 5723-5737 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Intermediary metabolism Gluconeogenesis Dairy cows Grazing Holstein-Friesian |
| dc.title.none.fl_str_mv | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_0dda355cf1fa4b6b777c2f514bce0c02 |
| identifier_str_mv | García-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/43074 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based systemGarcía-Roche Saracco, María MercedesTalmón Travers, DanielCañibe, GuillermoAstessiano, Ana LauraMendoza, AlejandroQuijano, DanielCassina, AdrianaCarriquiry Fossemale, MarianaIntermediary metabolismGluconeogenesisDairy cowsGrazingHolstein-FriesianThe objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures.SpringerGarcía-Roche Saracco María MercedesTalmón Travers DanielCañibe GuillermoAstessiano Ana LauraMendoza AlejandroQuijano DanielCassina AdrianaCarriquiry Fossemale Mariana2024-03-14T12:30:59Z2024-03-14T12:30:59Z2022Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfGarcía-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737https://hdl.handle.net/20.500.12008/43074https://doi.org/10.3168/jds.2021-21358reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengJournal of Dairy Science, 105(7): 5723-5737Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/430742026-04-14T10:08:59Z |
| spellingShingle | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system García-Roche Saracco, María Mercedes Intermediary metabolism Gluconeogenesis Dairy cows Grazing Holstein-Friesian |
| status_str | publishedVersion |
| title | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| title_full | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| title_fullStr | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| title_full_unstemmed | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| title_short | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| title_sort | Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system |
| topic | Intermediary metabolism Gluconeogenesis Dairy cows Grazing Holstein-Friesian |
| url | https://hdl.handle.net/20.500.12008/43074 https://doi.org/10.3168/jds.2021-21358 |